Developmental validation of a Cannabis sativa STR multiplex system for forensic analysis

A developmental validation study based on recommendations of the Scientific Working Group on DNA Analysis Methods (SWGDAM) was conducted on a multiplex system of 10 Cannabis sativa short tandem repeat loci. Amplification of the loci in four multiplex reactions was tested across DNA from dried root, stem, and leaf sources, and DNA from fresh, frozen, and dried leaf tissue with a template DNA range of 10.0-0.01 ng. The loci were amplified and scored consistently for all DNA sources when DNA template was in the range of 10.0-1.0 ng. Some allelic dropout and PCR failure occurred in reactions with lower template DNA amounts. Overall, amplification was best using 10.0 ng of template DNA from dried leaf tissue indicating that this is the optimal source material. Cross species amplification was observed in Humulus lupulus for three loci but there was no allelic overlap. This is the first study following SWGDAM validation guidelines to validate short tandem repeat markers for forensic use in plants.     

SOUTH ASIA

For nearly a hundred years, to many foreigners “Peking” meant the foreign legation quarter and its colourful western inhabitants. The article gives details of the extraordinary life they led a stone's throw from the Emperor's palace in a city that remained virtually untouched by the modern world. After the Boxer Rising of 1900, the legation quarter became a Treaty Port with its own laws and administration. That status continued through the First World War and beyond. But just as the foreigners were at last beginning to value Peking's uniqueness, the end was in sight. Life changed a lot after the 1937 Japanese invasion of China and, for many, internment followed the attack on Pearl Harbour. But the end came when Treaty port status was abolished for good at the end of the War     

Forecasting the 2013 German Bundestag Election Using Many Polls and Historical Election Results

This article reports on an attempt to forecast the outcome of the 2013 election to the German Bundestag. In contrast to the predominant academic approach to forecast incumbent vote shares from measures of government popularity, economic conditions and other fundamental variables, we entirely relied on data from published trial heat polls. Opposite to common practice in the news media, we did not take isolated polls as election forecasts in their own right. Instead, we used historical data to assess empirically the relationship between polls and election outcomes, and combined extrapolations from current polls in a Bayesian manner. The forecast was published one month ahead of the election. The retrospective evaluation of our method was added after the election. While our method is parsimonious and provides a large lead time, the performance at the 2013 election was underwhelming. We offer additional suggestions how the approach can be improved in future scenarios.     

The legitimization and institutionalization of ‘parental alienation’ in the Province of Quebec

ABSTRACT

This article examines the legitimization and institutionalization of ‘parental alienation’ discourse in the Province of Quebec, Canada. It draws upon an analysis of 31 documents (legislation, research reports and articles, training documents, professional documents and media articles) and interviews with 13 key informants, who were selected based on their knowledge of ‘parental alienation’ in research, policies or practices. The research findings reveal that the legitimization and institutionalization of ‘parental alienation’ discourse is a more recent process than in other provinces and countries, but that it has now permeated child custody as well as child protection proceedings. Academic researchers and media have been instrumental in this legitimization and institutionalization process, while the role played by changes to child protection policies is more ambiguous. The findings reveal researchers’ and experts’ tendency to distance themselves from Gardner’s controversial work on ‘parental alienation syndrome’ and to address the critiques by proposing new approaches and new concepts. However, the terms ‘parental alienation syndrome’, ‘parental alienation’ and ‘alienating behaviours’ are often used interchangeably, and assessment practices tend to rely on similar indicators.