Developmental validation of reduced-size STR Miniplex primer sets

This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic casework, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification conditions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA.     

Cross-examination of liquid-liquid extraction (LLE) and solid-phase microextraction (SPME) methods for impurity profiling of methamphetamine

Impurities in 48 methamphetamine (MA) samples were analyzed by liquid-liquid extraction (LLE) and headspace solid-phase microextraction (HS-SPME) methods. MPS-2 autosampler was used to improve reproducibility of SPME method, and nonadecane (C(19)) diluted with potassium bromide (KBr) powder was used as an internal standard for standardizing retention time. Impurities identified by SPME method showed different patterns compared with LLE method. Non-volatile impurities like methamphetamine dimer were not identified by SPME method, but some volatile impurities like diphenylketone, caprolactam and lots of unknowns were identified only by SPME method. 1-Phenyl-2-propanone (P2P), 1-phenyl-2-propanol and benzylcyanide peaks could be discriminated clearly by SPME method without interference of amphetamine, an artifact originates from MA degradation. Differences in the impurity patterns resulted in different clustering results. When 48 MA samples were classified into 5 LLE and 5 SPME clusters, cross-matching of the clusters resulted in 8 sub-clusters. It shows that combination of the different extraction methods can distinguish the differences which cannot be distinguished by LLE or SPME method alone, and can improve reliability of the profiling results.     

Developing a sustainable competitive advantage: absorptive capacity,knowledge transfer and organizational learning

This study proposes a theoretical model to investigate a sustainable competitive advantage (CA) by developing the absorptive capacity (AC), knowledge transfer (TR), organizational learning (OL) and in Taiwan’s financial and high technology industries. Structural equation modeling is employed to examine the influence between each variable and whether their relationships are varied in these two different industries. The study population comprises the top 100 financial and top 1000 high-technology enterprises in Taiwan published by Common Wealth Magazine in 2012. A total of 345 valid responses were collected. The research results indicate that OL serves as partial and full mediators between AC, TR and CA respectively. In addition, moderating effect exists in different industries on the theoretical model. Finally, discussion and implications are described for concluding research findings.     

Changes and continuities. Evolution of a Chinese family business (1876–2004)

Starting from the early nineteenth century, western colonial activities have opened up a large area of Southeast Asia for economic penetration. Chinese family business, with its extensive familial and cultural networks, has a niche in these frontier areas where economic and legal institutions were embryonic or ineffective. In Southeast Asia, Chinese extended families are often geographically dispersed. By spreading wealth across borders, these families have not only diversified their business risk, but also built up a mechanism to enforce business obligations cross borders. Contractual obligations in business could always be enforced by one’s familial and communal mechanisms without recourse to legal authority or institutions outside the communities. It helps to explain why the Overseas Chinese communities, over the centuries, have played an important part in the ties which China has forged with its neighbouring regions in Asia. By looking into the historical transformation of a traditional Chinese family business under five generations of patriarchal leadership, this article intends to examine the validity of the above thesis. Eu Yan Sang (EYS), a famous manufacturer and retailer of Chinese medicines over the past 120 years, is the focus of this study.     

Southern Green Cultural Criminology and Environmental Crime Prevention: Representations of Nature Within Four Colombian Indigenous Communities

Critical Criminology - This exploratory study develops a “southern green cultural criminology” approach to the prevention of environmental harms and crimes. The main aim is to...     

Use of hand-held laser scanning in the assessment of craniometry

The intra- and inter-examiner reliability was evaluated for hand-held 3D laser scanning technology when it was combined with localization of landmarks for craniometry. The data from the laser surface scanning were compared with those of conventional direct measuring. Using thirty unidentified skulls requested for individual identification, measurements were taken of the line distances from lambda to 26 landmarks, and also for seven breadth parameters. For the laser surface scanning, two examiners performed replicate measurements with an interval of 1 week. In the conventional direct measuring, the first examiner took replicate measurements with a 1-week interval. To assess intra- and inter-examiner reliabilities, the intraclass correlation coefficient was used. Analysis of variance with repeated measures for each parameter was performed to compare the conventional method with the 3D scanning method. Both the 3D scanning and conventional methods showed excellent intra-examiner reliabilities, and the 3D laser scanning method also showed excellent inter-examiner reliability. A statistical difference between the two examiners was found only in nasal breadth in the 3D laser scanning method. There was no significant difference between the two measuring methods, though the 3D laser scanning method tended to give a slightly lower reading. Collectively, the 3D laser scanning method with point localization is a useful method with excellent reliability, and it can replace the conventional direct measuring method in craniometry.     

韩国公务员培训的特点及启示

韩国在公务员培训方面具有培训管理的法制化、培训机构的多元化、培训种类的多样化、培训理念的先进性、培训内容的丰富性、培训方式的灵活性、培训成绩的权威性以及培训时间的机动性等特点。学习借鉴韩国公务员培训的经验,对我国公务员培训尤其是对学院进一步提高干部教育培训的科学化水平有很大的启示。