Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB)

Several assays for gamma-hydroxybutyrate (4-hydroxybutyrate, GHB) have been developed based on the enzyme gamma-hydroxybutyrate dehydrogenase (GHB-DH). Enzymatic oxidation of GHB by NAD+ is coupled to diaphorase-mediated reduction of pro-dye to yield colored product. GHB-DH from Ralstonia eutropha was cloned and expressed as a stable fusion protein easily purified by affinity chromatography. Quantitative initial velocity and endpoint versions of the assay in solution are described. Michaelis-Menten parameters for oxidation of GHB and ethanol were estimated. A semi-quantitative "dipstick" version of the assay on paper also is described. Both solution endpoint and "dipstick" assays are sensitive to about 0.05 mg GHB/mL using 10 microL of sample. Ethanol at concentrations possible in urine and agents used to stabilize physiological fluids for forensics analysis do not interfere significantly. The "dipstick" assay also allows detection of GHB in alcoholic beverages after evaporation of about one-fourth drop of beverage before testing. The enzymatic assay for GHB is reliable, sensitive, inexpensive and rapid.     

Psychological variables in student activism: The radical triad and some religious differences

Thirty-six male students, drawn from a sample of 1195, were interviewed to obtain a personal history. A battery of projective psychological tests (Rorschach and TATs) were also administered to them. The students were divided into four groups of nine each, Jewish radicals (JR), Christian radicals (CR), Jewish moderates (JM), and Christian moderates (CM), to test the significance of religious background as it related to political outlook. Eight significant psychological variables were found and defined. No differences were found between JMs and CMs. Radicals differed from moderates on three variables: negative identity, masochistic surrender, and treating people as concepts. In addition, JR subjects demonstrated consistently a wandering fantasy, flight from the mother, the mother as salient, and machismo as psychological variables. CRs were not characterized by any of these variables. As with both groups of moderates, the father of the CRs was psychologically salient, but unlike the moderates, CTs perceived their fathers as flawed. The possible dynamic meaning of these configurations is discussed, as are their possible relationship to radical behavior and radical political ideology.This study was supported by grants from the American Jewish Committee and The National Science Foundation (GS35307A).Director of Resident Education, McLean Hospital. M.D., Harvard Medical School; residency training at Boston Veteran's Administration Hospital and Beth Israel Hospital in psychiatry. Psychoanalytic training, Boston Psychoanalytic Institute. Major interest: depression.Director of Training for Psychology Interns. Ph.D., Brandeis, 1960; Master's degree, University of Illinois. Major interest: schizophrenia.Professor of Political Science, Smith College. Major interest: modern European history — applying psychoanalytic methods to historical and social problems like student activism.     

B. Reviews and notes of recent bibliographies

i. Le Moyen‐Orient contemporain. By Guy Feuer, with the collaboration of S. Chauvin and F. Pourcelet. (Guides de Recherches, No. 6) Paris, Fondation Nationale des Sciences Politiques, 1975. pp. 245. 48 fr.

ii. Eléments de bibliographie des études arabes. By Aubert Martin. Paris, Publications Orientalistes de France, 1975. pp. iii, 235. 35 fr.

iii. The Emergence of Arab Nationalism from the 19th century to 1921: A Bibliography. Compiled and annotated by Frank Clements, London, Diploma Press, 1976. pp. x, 290. £10.00

iv. Union catalogue of Arabia Serials and Newspapers in British Libraries. By the Middle East Libraries Committee, edited by Paul Auchterlonie and Yasin H. Safadi. London, Mansell, 1977. pp. xvi, 146. £12.50.

v. Arabia Historical Writing, 1973 & 1974. By Fawzi Abdulrazak. Cambridge, Mass., Harvard University Library, 1974 & 1976. 2v. $ 8.65 (for both vols).

vi. Modern Arabic Literature: A Bibliography of Articles, Books, Dissertations and Translations in English. By Salih J. Altoma. Bloomington, Indiana University, Asian Studies Research Institute, 1975. pp. 73. $ 4.00

vii. The Kurds in Iran, a Selected and Annotated Bibliography. By Wolfgang Behn. London, Mansell, and Munich, Verlag Dokumentation, Publishers, 1977. pp. 76. £6.00

ix. American Doctoral Dissertations on the Arab World 1883–1974. Second edition. By George Dimitri Selim. Washington D.C., Library of Congress, 1976. pp. xviii, 173. Obtainable from: Superintendent of Documents, Government Publications Office, Washington D.C. 20402. Price: outside USA $5.75 plus $1.44 for overseas postage.

x. International and regional politics in the Middle East and North Africa: a guide to information sources. (International Relations Information Guide Series, 6). By Ann Schulz. Detroit, Gale, 1977. pp. xii, 244. $18.00

xi. The politics of African and Middle Eastern states: an annotated bibliography. By Anne Gordon Drabek & Wilfrid Knapp. Oxford, Pergamon, 1976. pp. x, 192. £6.75     

Treasury Department regulatory material under the tax code

The legislative process in the tax field and the administrative process for the issuance of regulatory and interpretative material by the Treasury Department are closely coordinated. The same persons and interests both inside and outside of Government are involved in both processes. There is thus continuity of expertise, understanding of what is at stake, adversary viewpoints, and Washington knowhow. The preparation of administrative material within the Treasury is a task for those technical personnel and policy officials who in varying degrees also have a voice in the preparation of the Treasury's legislative position and the legislative development of statutory language. The outside forces who watch legislative activity also closely watch administrative activity and seek to shape the latter just so they do the former. The response of the Treasury is much the same in both situations, and governed by the policies, attitudes and predilections of tax policy officials and career personnel.     

A New Concept of the Citizen-Soldier

The military manpower demands created by U.S. operations in Afghanistan and Iraq are causing extraordinary strains on recruitment, and the desirable size of the U.S. armed forces has become a topic of national concern. One key issue is the balance between active-duty forces and the reserve components, citizen-soldiers who face numerous service-related issues that the active-duty forces do not and who have their own unique sociology. We cannot continue to rely on the reserves for missions such as Iraq and Afghanistan, but must look to the use of short-term active-duty enlistments, drawn from recent college graduates, to perform duties currently being performed by reservists and even active-duty personnel.     

American Grand Strategy in a World at Risk

If there is no single long-standing American grand strategy, one nonetheless sees through the course of U.S. history the tracks of a grand strategy. It started with the idea of a U.S. monopoly in the Western Hemisphere, along with balances of power in the chief theaters of the world; with belief in the primacy of sea and air power and the need for an economic system to support these; and the objective of transforming international politics. Since 9/11, even if the strategic hierarchy, intensity, and political basis have changed, the Bush administration has largely been continuing in this same project, with a sensible strategy but poorly considered tactics.     

Outsourcing – Financial Services Authority report on offshoring

This article evaluates the 2005 Financial Services Authority report into offshore outsourcing with specific reference to experience in India. It concludes that, on the whole, the findings are positive and that UK business methods appear to transfer well.