The application of an automated allele concordance analysis system (CompareCalls) to ensure the accuracy of single-source STR DNA profiles

A powerful method for validating a scientific result is to confirm specific results utilizing independent methodologies and processing pathways. Thus, we have designed, developed and validated an automated allele concordance analysis system (CompareCalls, patent pending) that performs comparisons between two independent DNA analysis platforms to ensure the highest accuracy for allele calls. Application of this system in a quality assurance role has shown the potential to eliminate greater than 90% of the STR analysis required of a DNA data analyst. While this system is broadly applicable for use with any two independent STR analysis programs, either prior to or following human data review, we are presenting its application to data generated with the ABI Prism Genotyper software system versus data generated with the SurelockID system. With the automated allele concordance analysis system, the GeneScan DNA fragment data generated from an ABI 377 gel image are analyzed in two independent pathways. In one analysis pathway, the GeneScan data are imported into Genotyper software where STR labels are assigned to the fragment data based upon the criteria of the Kazam 20% macro. The "Kazam" macro provided with the Genotyper program works by labeling all peaks in a category (or locus) and then filtering (or removing) the labels from peaks, such as those in stutter positions, that meet predefined criteria. In the second pathway, the GeneScan data are imported into the SurelockID analysis platform where STR labels and error messages are assigned to the fragment data based upon hard-coded allele calling criteria and quality parameters. The resulting STR allele calls for each analysis platform are then compared, utilizing the automated allele concordance analysis system. Any differences in the STR allele calls between the two systems are flagged in a discordance report for further review by a qualified DNA data analyst. The automated allele concordance analysis system guides the DNA data analyst to the discordant data generated by either analysis platform. Additionally, the analyst is also directed to data that are of less than pristine quality which may have an increased potential for errors in interpretation by either analysis platform or by a human DNA data analyst. Implementation of an automated allele concordance analysis system will yield high-quality data for CODIS and free the human DNA data analyst to perform other critical duties within the laboratory.     

What's looks got to do with it? Instructor appearance and student evaluations of teaching

Some studies suggest that instructors' looks are important determinants of their Student Evaluations of Teaching (SET) results. Yet the studies examining this proposition leave out many independent variables that have been found to be important determinants of SETs in the SET literature. This study adds in most such factors and finds that, controlling for these other factors, attractiveness has no effect in our data. We conclude that earlier findings indicating that instructor appearance is important are likely due to omitted variable bias. © 2005 by the Association for Public Policy Analysis and Management     

Let Them Eat Marblecake: The Preferences of Michigan Citizens for Devolution and Intergovernmental Service-Provision

Based on a survey of Michigan residents, this study explorespublic support for devolution of public services to state andlocal governments. The survey allowed respondents to choosemore than one level of government to provide services. On onehand, a large majority endorsed the devolution of services fromthe federal government to the states in general. On the otherhand, for most of 11 specific services studied, respondentspreferred arrangements involving all three governments. Regressionanalysis indicates that a preference for having services deliveredby state and/or local governments only is stronger among Republicans,conservatives, whites, and older citizens. Overall, however,Michiganians embrace "marblecake " federalism.     

Dependency,Self-Criticism,and Mothers' Responses to Adolescent Sons' Autonomy and Competence

A previous study found that dependency was associated with mothers' reacting punitively to independent behavior of their adolescent daughters and that self-criticism was associated with punitive and controlling responses to adolescent daughters. To extend these findings to mother–son relationships, the present study examined relations between personality and parenting behavior in 78 mothers of adolescent boys. Mothers completed measures of dependency and self-criticism and of baseline affect, and then were given experimentally manipulated results of the son's initial assessment of problem-solving ability (competence) and choice of discussion partner. Affect was assessed again. Mothers coached sons on computer problem-solving puzzles. Dependency was associated with mothers' giving fewer explicit commands and less negative feedback to (1) sons who were described as high in competence and who chose someone else as discussion partner and (2) sons who were described as average in competence and who chose the mother as discussion partner. It was concluded that mothers high in dependency relate to competent sons in ways that encourage independence, but relate to their less competent sons in ways that may foster dependency, by thwarting attempts at autonomy.     

Lessons from Analogical Reasoning in the Teaching of Negotiation

After two decades of spectacular growth in negotiation research, teaching,and application, it is appropriate to pause and consider how negotiatorslearn, a strikingly fundamental but infrequently examined issue. In thisreport, we present some initial and on-going research and thinking onlearning about negotiation skills, providing one view of the relationshipbetween negotiation pedagogy and negotiation practice.     

Social context and the subjective experience of different types of rock music

The psychological impact and meaning of rock music for adolescents needs to be addressed as a joint function of the type of music and the social context in which it is listened. Young adolescents in this study carried pagers for one week and reported their affect, arousal, and psychological involvement in their activities, including music listening, when signaled at random times. Regression analyses indicate that social context has its greatest impact on soft rock where subjective states are dramatically higher when this music is listened to with friends, as opposed to alone. This suggests that intimate companionship transforms the themes of longing and sadness characteristic of this music into a more positive and binding experience. Subjective states experienced listening to this music are also high when listening occurs in the bedroom, a finding that indicates a consistency between the music's themes and the use of the bedroom as a place for contemplation and reflection. Top 40 and hard rock/heavy metal music are experienced positively across a variety of social contexts, suggesting that their energy and collective message allows teens to experience mastery and power over their environment, even in contexts that are ordinarily lonely and alien. The one exception is when hard rock/heavy metal is listened to in the presence of family members, a situation that creates very negative subjective states. This is undoubtedly due to the dissonance of this music's themes with parental values.This research was carried out at Michael Reese Hospital in Chicago with a grant to Reed Larson from the National Institute of Mental Health (No. MH38324).He received his M.A. from University of Windsor. He is in the final stages of completing a Ph.D. dissertation investigating the role the news media play in contributing to selected social and political attitudes toward AIDS.He received Ph.D. from the Committee on Human Development, University of Chicago. His research focuses on daily experience associated with health and psychopathology in adolescence and across the life span.     

Pension coverage and benefits, 1972: findings from the retirement history sutdy

This article, based on data from the Retirement History Study, examines coverage by an employee pension plan on the longest job and the extent to which covered workers received an employee pension upon retirement and the size of their benefits. It also examines the joint receipt of employee pension and OASDI benefits and the size of the combined benefits. Each of these pension variables is analyzed for differences by class of worker (private wage and salary or government), sex, and characteristics of the longest job (industry, occupation, tenure, recency of job, extent of employment, and annual earnings rate). The majority of completely retired individuals in their early to middle sixties in 1972 did not receive employee pension benefits in that year. Women employed in private industry on their longest job were the most disadvantaged in this regard. Even when they were fortunate enough to receive retirement benefits from employee pension plans, their benefits were substantially lower than those of men or of women employed in government.     

Populisms and liberal democracy – business as usual?

Populism is often thought to mark a sharp break with liberal democracy. But to what extent is this the case? In this contribution the connections between populism and liberal democracy are sketched in the context of several areas where discussions about populisms have stressed their discontinuity with the liberal democratic tradition and its practices. The discussion concentrates upon the notions of the people, their representation, elites, constitutional issues and economic policies. In each case it is suggested that continuities are as strong, and sometimes stronger, than discontinuities in these relationships. The contribution ends with a discussion of how it might be possible to defend liberal democracy from a non-liberal position in the face of the critique from populisms.