Discovery and identification of new D13S317 primer binding site mutations

During the course of conventional testing of CODIS standards at the Alabama Department of Forensic Sciences, a sample with a heterozygous null genotype at D13S317 was discovered using the PowerPlex 1.1 kit (Promega, Madison, WI). The loss of both alleles was confirmed when the sample was amplified using PowerPlex 1.2 primers and resulted in a 9, 10 genotype at this locus. To determine the cause of the silent alleles, the ADFS designed D13S317 primers which encompassed the PowerPlex 1.1 D13S317 primer binding sites and sequenced the region. Both alleles showed the presence of two substitutions (T-->A and G-->T) at positions 1 and 5 (5'-->3') of the reverse primer (positions 196 and 200 of the sequence in GenBank accession number ). Since the mutations were identical on both alleles, they may be assumed to be of ancestral origin.     

A poverty of distinction

Homogenizing the poor, treating them as a shapeless blob, is not only dehumanizing but also makes them candidates for a single set of public policies. We propose to show, instead, using cultural theory, that there are different types of poor people, who follow different strategies in coping with or overcoming their condition.     

Mainstreaming: A case for optimism and cynicism

This paper seeks to evaluate the concept of mainstreaming against a range of feminist critiques of laws and legal systems and to examine the case for the pursuit of feminist politics through mainstreaming strategies. It begins, in section two, by identifying theme sin existing mainstreaming literature, and then in section three considers the potential of mainstreaming to tackle the causes o fine quality. In particular it questions whether mainstreaming can address the patriarchal nature of laws and legal systems and the essentialising tendencies of law, and whether mainstreaming can effectively tackle market-driven inequality. A final section considers the conditions under which feminists might consider engagement with mainstreaming and the limits of such strategies. This revised version was published online in July 2006 with corrections to the Cover Date.     

Effects of marital conflict and family structure on the self-concepts of pre- and early adolescents

This study explored the effects of marital conflict and family structure on the self-concepts of youth aged 8–12. Subjects were 16 mother-youth pairs from intact parent families (IP) and 17 mother-youth pairs from separated parent families (SP). Results revealed youth in families with low marital hostility and high marital affection had significantly more positive self-concepts. Analyses suggested differences between IP and SP groups may best be explained by differences in marital hostility. The authors discuss the effects of marital discord on parenting within an ecosystems perspective.This study received partial support from the Indiana University School of Nursing Alumni Association and Sigma Theta Tau, Alpha Chapter.Research interests: children's social networks, marital structure, and parenting.Research interests: chronic illness in children.