Alleles responsible for ABO phenotype-genotype discrepancy and alleles in individuals with a weak expression of A or B antigens

ABO types obtained from evidentiary samples have been used effectively to obtain the initial information leading to the apprehension of culprits in Japanese criminal investigations. A simple ABO genotyping method using multiplex sequence-specific PCR and capillary electrophoresis was developed as a supplement to serological ABO typing. Limitations in predicting a phenotype based on genotype were evaluated using 1134 randomly selected Japanese peripheral blood samples. A concordance rate of 99.82% (1132/1134 samples) was found between genotypes and phenotypes defined as Groups A, B, AB, and O. Sequencing analysis revealed that one discrepant sample contained an O allele having a previously unreported point mutation at the primer binding site in exon 6, and another discrepant sample contained an O allele lacking the guanine deletion at nt 261 (the O301 allele). Therefore, the existence of such alleles must be given some consideration when predicting phenotype based on genotype.     

Hippuric acid and methyl hippuric acid in rat hair: possible monitoring of xylene and toluene exposure

Thinner is mainly composed of toluene and xylenes, and we studied the incorporation of the main metabolites of toluene and xylenes, hippuric acid (HA) and o-, m-, and p-methyl hippuric acids (o-, m-, p-MHA), in dark agouti rats' hair. Rat black hair was shaved before any exposure with an electric shaver designed for animals. Studies were performed in vivo with exposures of 30 min per day at three different concentrations (100, 300, and 1000 ppm) of toluene and o-, m-, and p-xylene for a total of 10 times over 2 weeks. Newly grown hair was tweezed out from the root with tweezers at seventh of the last exposure. Hair samples were then washed, extracted, derivatized, and analyzed by gas chromatography-mass spectrometry (GC-MS). HA and o-, m-, and p-MHA were not detected (ND) in the unexposed rat hair. After exposure, the metabolite concentration in the hair changed depending on the exposure concentration. Mean concentrations ranged from ND to 7.6 ng/mg, from ND to 13.8 ng/mg, from ND to 10.1 ng/mg, and from ND to 9.2 ng/ml hair for HA, o-, m-, and p-MHA, respectively. These results indicate that the metabolites concentrations in hair are effective indices of thinner exposure.     

Power to make land dispossession acceptable: a policy discourse analysis of the Merauke Integrated Food and Energy Estate (MIFEE), Papua,Indonesia

The Merauke Integrated Food and Energy Estate (MIFEE) in Papua, Indonesia, is a state-led mega-project to transform local agriculture through large-scale corporate investment in food crops and biofuels for foreign markets. The project has led to extensive land dispossession, accompanied by devastating social and ecological impacts. This contribution analyzes how discourse regarding food and energy crises has been employed to release land from customary tenure to a coalition of state, corporate and local elite actors. The interests of these actors have converged on the state-led mega-project to transform local agriculture through large-scale corporate investment in food crops and biofuels in the name of national food security.     

The uniqueness of pure-strategy Nash equilibrium in rent-seeking games with risk-averse players

This article proves that if each player’s measure of absolute risk aversion is nonincreasing in his/her wealth, then there exists a unique pure-strategy Nash equilibrium in the general rent-seeking game, where risk-averse players can coexist with risk-neutral players, attitude toward risk can differ among players, and concave production functions for lotteries are not necessarily the same among players.     

Methamphetamine body packer: acute poisoning death due to massive leaking of methamphetamine

We encountered three methamphetamine (MA) body packers presenting simultaneously, one of whom died. Three Nigerian men (39, 35, and 37 years old) who attempted to smuggle were found to contain 35 (498 g), 21 (292 g), and 5 packages (73 g) of methamphetamine hydrochloride (MA-HCl) in their stomachs, respectively. Packages were wrapped with plastic film and Scotch tape. The 39-year-old man died with acute poisoning from c. 20 g of MA-HCl that had leaked from the packages into the stomach. His plasma MA concentration was 8.6 microg/mL when he was hospitalized (17 h before his death). Autopsy findings showed extreme pulmonary congestion and edema as well as moderate hepatic edema and several petechiae. Quantitative analysis was performed by gas chromatography/mass spectrometry. Extremely high concentrations of MA and its metabolite amphetamine (AP) were found in cardiac blood (63.5 microg/mL and 1.2 microg/mL), urine (4,518 microg/mL and 72.4 microg/mL), gastric contents (8,490 microg/mL and 16.9 microg/mL), and in all other autopsy samples. These high concentrations confirmed that the cause of death was acute MA poisoning. Furthermore, impurity-profiling analysis of the seized MA revealed that the MA smuggled by the three suspects originated from the same batch.     

Politics of childcare policy beyond the left–right scale: Post‐industrialisation,transformation of party systems and welfare state restructuring

Childcare policy has become an integral part of social and economic policy in post‐industrial democracies. This article explores how the transformation of party systems structures the politics of childcare policy. It reveals that political parties contend with each other over childcare and female employment policy on the social‐value dimension as well as the redistributive dimension. Assuming that different party policies have distinct impacts on public childcare policy, it is hypothesised in this article that a government's policy position – composed of the governing parties' policy positions – affects changes in public spending for childcare services. Through an analysis of the pooled time‐series and cross‐section data of 18 advanced industrialised countries from 1980 until 2005 using multivariate regression methods, it is revealed that a government's redistributive left–right policy position interacts with its social liberal–conservative policy position, and that a left–liberal government raises its budget for childcare services while a left–conservative government does not.     

日本社会的结构变化与高等教育的课题

20世纪 90年代 ,日本面临着全球化背景下知识社会的新挑战。因此 ,日本的高等教育不但一方面要把注意力集中在其中的一些结构性问题上 ,同时要满足来自于经济和社会变革的需求。日本政府为此提出了一系列高等教育改革措施。东京大学正在落实的改革有三个方面。第一是管理 ,包括学校管理和财政。目前 ,政府倡导要求国立大学具有更大的自主性 ,给校长更大的权力。虽然这似乎是一种普遍的方向 ,但是 ,政府详细的改革计划也带来了各种各样的问题。第二个改革领域是研究。大学采取措施加强与工业界的关系 ,加强国际关系网络。第三 ,教育 ,特别是本科生层次的教育是大学非常重要的任务之一。然而 ,在一个以研究为中心的大学 ,提高教育质量不是一件容易的事。东京大学已经着手采取措施改进本科生教育     

Determination of a newly encountered designer drug "p-methoxyethylamphetamine" and its metabolites in human urine and blood

A newly synthesized designer drug, para-methoxyethylamphetamine (PMEA) was unexpectedly detected in the postmortem specimens of fatality involving drug intoxication in 2005, Japan. For unequivocal identification, the isomeric discrimination of PMEA and its positional-isomers was performed by GC/MS with the trifluoroacetylation. In order to prove the intake of PMEA, the characteristic metabolites of PMEA were also identified by GC/MS analysis of the urine specimen with trifluoroacetylation. As a result, para-methoxyamphetamine, para-hydroxyethylamphetamine (POHEA) and para-hydroxyamphetamine were identified as the major metabolites of PMEA. For the quantitative analyses of PMEA and its three metabolites in body fluids, an automated column-switching LC/MS procedure was developed, and applied to the postmortem blood and urine specimens. In this fatal case, blood concentration of PMEA was estimated to be 12.2 microg/mL and this level seemed extremely high in comparison with lethal blood-levels of its analogues, representing acute-intoxication of the victim. Based on the quantitative results, PMEA was found to be extensively metabolized to POHEA via O-demethylation, partly followed by its conjugation.     

The Constitution of Japan and Constitutional Reform

Although there have been movements to revise the Constitution of Japan, the original text has remained in effect for 70 years. This article examines the rules of constitutional revision, the basis on which the Constitution was drafted, and the ramifications of an unmodified constitution. Japan's Constitution is compared in terms of length and number of revisions with those of other industrialized countries. The article concludes with recommendations for making Japan's Constitution better able to function in a legal capacity.