The hidden electoral connection: analysing parliamentary questions in the Chilean Congress

Legislators in presidential countries use a variety of mechanisms to advance their electoral careers and connect with relevant constituents. The most frequently studied activities are bill initiation, co-sponsoring, and legislative speeches. In this paper, the authors examine legislators’ information requests (i.e. parliamentary questions) to the government, which have been studied in some parliamentary countries but remain largely unscrutinised in presidential countries. The authors focus on the case of Chile – where strong and cohesive national parties coexist with electoral incentives that emphasise the personal vote – to examine the links between party responsiveness and legislators’ efforts to connect with their electoral constituencies. Making use of a new database of parliamentary questions and a comprehensive sample of geographical references, the authors examine how legislators use this mechanism to forge connections with voters, and find that targeted activities tend to increase as a function of electoral insecurity and progressive ambition.     

Robustness of the Y STRs DYS19, DYS389 I and II, DYS390 and DYS393: optimization of a PCR pentaplex

Various technical methods were investigated with the aim of developing a multiplex system to amplify five Y-chromosome STR loci in the same PCR reaction: DYS393, DYS19, DYS390, DYS389 I and DYS389 II. A sequenced allelic ladder was constructed with previously sequenced alleles including the most common ones. A number of reamplification conditions of the allelic ladders were tested. The pentaplex was evaluated for typing using two different platforms (ABI and ALF) with promising results. However, in degraded samples non-specific artifacts were observed in the DYS393 system in the same range of sizes as the real alleles. This system can also be typed in females under relatively low stringency conditions in the PCR amplification, making this system prone to errors in critical samples. This lack of specificity can be reduced by increasing the stringency of the PCR conditions. The DYS19 ladder cannot be reamplified as stutters appear after a few reamplifications. These stutters are probably due to a 2 bp slippage induced by the presence of a TA repeat stretch in the PCR amplified fragments. Non-specific products were also noted in the DYS389 I and DYS389 II amplification, although out of the range of other alleles in this pentaplex. This newly constructed pentaplex has proved to be very useful in population genetic studies because all five Y STR markers can be loaded in the same lane of a gel with other Y STR singleplex or multiplexes. The usefulness of Y-chromosome STRs in criminal casework is especially evident in analyzing azoospermic individuals.     

Autoerotic asphyxial death initially misinterpreted as suicide and a review of the literature.

Death during autoerotic activities is of special concern to law enforcement officials, medical examiners, the family of the decedent, and society in general. As in the probing of any violent demise, accurate identification, management, and preservation of all physical evidence; complete photographic documentation of the scene and the body; reconstruction of the scene; and interviews with the family and acquaintances (psychological autopsy) are mandatory for proper study, evaluation, and interpretation of the case. Because of a lack of knowledge on the part of many people, including professionals, about these activities and the fact that many autoerotic fatalities share common characteristics with suicide and homicide, these cases are often misinterpreted. The authors present a case of autoerotic accidental asphyxial death which was initially misinterpreted as suicide.     

New EU Members in Europe's Security Policy

What impact will the 2004 round of enlargement have on the European Union's common foreign and security policy? This article argues that the new members' arrival in theory strengthens the Euroatlantic camp within the EU. This impact has, however, been limited by the accession states' difficulty in exercising effective influence in Brussels so far, and their foreign policy is also coming under pressure at home because of the unpopularity of the Iraq War. The newcomers hold distinct views on the EU's policy toward Russia and other countries of the former Soviet Union. On this point, their efforts have begun to slowly but demonstrably transform the European Union's own involvement in countries on the EU's eastern frontier. In addition, the first signs are becoming apparent of the new members' effective imprint on the EU's common foreign and security policy.     

The post mortem alterations of myofibrilar proteins and adenosintriphosphatase activities of the skeletal muscles (author's transl)]

Immediately after the death of rabbits and at different times within 48 hours, we took out a part of the psoas muscle, from which we made myofibrilar preparations. The carcasses providing the muscle samples were held at two different temperatures. One group was held for 48 hours at 25 degrees C, imitating room temperature. The other group was held for 12 hours at 25 C degrees and at 25 C degrees and 12 hours at 10 C degrees, imitating daily temperature changes. Each myofibrilar sample was subjected to SDS-polyacrylamide gel electrophoresis. In addition we determined the Ca++ activated and the EGTA inhibited ATPase specific activity of the myofibrils. We found that within 48 hours the myofibrilar proteins were subjected to some characteristic proteolytic changes, which were dependant on the environmental temperature. The most interesting change was found in carcasses held constantly at 25 C degrees for 48 hours, where the EGTA inhibited ATPase activity was increased to about seven times its initial value, reflecting impairment of the troponin complex.