Elucidation and chemical identification of a heart strength and heart rate-increasing substance from human kidney extracts

Bioassays involving the measurement of cardioactivity have been used in the past to determine glycoside concentration in post-mortem specimens following glycoside poisoning. This paper describes the presence of a cardioactive principle in alcoholic extracts of kidney that could interfere with these bioassays and which has been identified as tyramine. For the analysis of tyramine a combination of purification methods was employed, including cation exchange with Sephadex CM C-25, gel filtration and HPLC. Detection was achieved using mass spectrometry after careful cation-exchange treatment. The fragmentation pattern with and without prior formation of the TFA-derivative corresponded to that tyramine. IR and UV spectra also indicated the presence of tyramine. Under suitable experimental conditions, positive inotropic and positive chronotropic effects on the right guinea-pig atrium and positive inotropic effect on the left guinea-pig atrium produced by the kidney extract can be shown to differ from those cardiac glycosides. These investigations bring into question earlier court decisions made in legal processes which have been based upon the results of bioassays for the evaluation of cardiac glycoside toxicity.     

Guatemala's Party Universe: A Case Study in Underinstitutionalization

The article examines the degree of institutionalization of the Guatemalan party universe across four areas: the pattern of inter‐party competition; the rootedness of parties in society; the legitimacy accorded to parties and democratic institutions; and the nature of internal party organization. Guatemala displays an extremely inchoate party structure across all these variables. There is no stability in the identity of the main parties in the polity. After more than two decades of electoral democracy, no single party has been able to avert a drift into electoral irrelevance or outright disappearance. With respect to the basic facets of internal party organization, Guatemalan parties exhibit a feebleness so pronounced that their very status as parties is questionable. In general, Guatemalan “parties” only fulfill Sartori's minimalist definition as organizations that field candidates for public office, but offer nothing more substantive.     

A new perspective in the estimation of postmortem interval (PMI) based on vitreous

The relation between the potassium concentration in the vitreous humor, [K+], and the postmortem interval has been studied by several authors. Many formulae are available and they are based on a correlation test and linear regression using the PMI as the independent variable and [K+] as the dependent variable. The estimation of the confidence interval is based on this formulation. However, in forensic work, it is necessary to use [K+] as the independent variable to estimate the PMI. Although all authors have obtained the PMI by direct use of these formulae, it is, nevertheless, an inexact approach, which leads to false estimations. What is required is to change the variables, obtaining a new equation in which [K+] is considered as the independent variable and the PMI as the dependent. The regression line obtained from our data is [K+] = 5.35 + 0.22 PMI, by changing the variables we get PMI = 2.58[K+] - 9.30. When only nonhospital deaths are considered, the results are considerably improved. In this case, we get [K+] = 5.60 + 0.17 PMI and, consequently, PMI = 3.92[K+] - 19.04.     

Detection of apoptosis in ischemic heart. Usefulness in the diagnosis of early myocardial injury

Myocardial samples of hearts with histologic findings of acute myocardial infarction (group A), sudden coronary deaths without histologic changes (group B), and chronic ischemic heart disease (group C) were analyzed to investigate the appearance of apoptosis in acute and chronic ischemic cardiac disorders. This analysis involved the morphologic detection of DNA strand breaks in myocyte nuclei by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and the biochemical determination of DNA laddering in the myocardium using archival formalin-fixed, paraffin-embedded tissue sections of human myocardium. The authors demonstrated that apoptosis of myocardial cells could occur after ischemic myocardial cell injury. In all documented cases of acute myocardial infarction (group A), the infarcted area included extensive presence of both apoptosis and necrosis. In the tissue bordering on and away from the obviously infarcted areas, positive nuclei were intermingled with nonstained normal myocytes. The number of positive nuclei decreased with the distance from the infarction foci. In group B, myocardial samples showed focal or diffuse nuclear positivity of varying degrees for apoptosis, confirming the presence of myocardial ischemic cell death, whereas the histologic diagnosis remained inconclusive. This finding suggests that apoptosis could be used as a marker for acute ischemic injury. In group C, stained nuclei were dispersed with intermingled normal cardiomyocytes.     

The Idea of the "Third Reich" in the German Legal, Philosophical and Political Thinking in the 20th Century

The idea that after the Nazi takeover the German political prpaganda machinestrongly supported the naming of their land the "Third Reich" (Drittes Reich) is a misperception shared by many historians, political scientists as well as lawyers all around the world even today. It is much less know that Hitler himself was never in full support of his expression even proved qiute effective both before and after NSDAP (Nationalsozialistische Deutsche Arbeiterpartei) tekeover.     

Deaths associated with electric shock injuries in children and adults: A 10-year retrospective examination in Şanlıurfa,Southeast Turkey (2012–2022)

Electric shock injuries (EIs) have high rates of morbidity and mortality in both children and adults. The aim of this study was to examine autopsy cases resulting from EIs and to compare pediatric and adult groups with respect to sociodemographic data, clinical characteristics, location of the incident, electricity entry–exit wound characteristics, current direction, pathological effects of the electric shocks on the body, and cause of death. A total of 8075 autopsies conducted between 2012 and 2022 were retrospectively examined. Şanliurfa is a city in the southeast of Turkey with the highest birth rate in the country. Of 123 cases of death resulting from EIs in Şanliurfa, 58 (47.2%) were children (age < 18 years). In the majority of children (81%), a low-voltage injury resulted from a domestic appliance while the children were playing at home in a rural area. In the vast majority of adults, a high-voltage injury (HVI) resulted from a workplace accident due to working with HVI materials in an area outside the home. The manner of death was accidental in all the electric shock injuries. The electricity entry and exit wounds formed as a result of EIs had similar characteristics in both children and adults, with no significant difference identified. In the province of Şanliurfa, Turkey, which borders Syria and where the main occupation is agricultural activities, there is a need to increase electrical safety precautions within the home, educate parents, and increase electrical safety conditions and worker education in the workplace.     

Sanitizing a crisis: The three dimensions of an enhanced regime of accumulation

In many western countries the Covid crisis has evolved from a public health crisis toward an economic crisis. Spain was no exception. Crises are always key moments in the reconfiguration of the role of the state, as this takes on new domains and functions. Conceiving state's role as a triad helps us understanding its functioning. From the initial stage, state work has been intense in order to mitigate its effects. But the Spanish particularity is the specific articulation of state apparatuses. As in previous crises, Spain has deployed a significant activity to police the public order. The policing of public spaces was tailored to ensure the economic apparatuses remain unchallenged. A new mode of regulation has been established to allow an enhanced regime of capital accumulation through different financial instruments. Despite the frequent neglect of ideological state apparatuses, this paper exposes how relevant they are in moments of impasse to produce a new moral economy reassuring the hegemonic project of the elites.     

Expert Reliability in Legal Proceedings: “Eeny,Meeny, Miny,Moe, With Which Expert Should We Go?”

Between Expert Reliability refers to the extent to which different experts examining identical evidence make the same observations and reach the same conclusions. Some areas of expert decision making have been shown to entail questions with relatively low Between Expert Reliability, but the disagreement between experts is not always communicated to the legal actors forming decisions on the basis of the expert evidence. In this paper, we discuss the issues of Between Expert Reliability in legal proceedings, using forensic age estimations as a case study. Across national as well international jurisdictions, there is large variation in which experts are hired to conduct age estimations as well as the methods they use. Simultaneously, age estimations can be fully decisive for outcomes e.g. in asylum law and criminal law. Using datasets obtained from the Swedish legal context, we identify that radiologists and odontologists examining knees or teeth images to estimate age seem to disagree within their own disciplines (radiologist 1 v. radiologist 2 or odontologist 1 v. odontologist 2) as well as across different disciplines (radiologist v. odontologist) relatively often. This may have large implications e.g. in cases where only one expert from the respective field is involved. The paper discusses appropriate ways for legal actors to deal with the possibility of lacking Between Expert Reliability. This is indeed a challenging task provided that legal actors are legal experts but not necessarily scientific experts.