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271.
A new STR typing strategy has been developed allowing the simultaneous amplification and subsequent analysis of 11 polymorphic systems with amplicon sizes smaller than 270bp. The multiplex amplification reaction includes six STR loci from the European standard set of loci (ESS) for DNA databases (D3S1358, D8S1179, D21S11, THO1, FGA and VWA) as well as four additional STR systems selected for their robustness (D2S1338, D12S391, TPOX and D5S818) together with the sex-specific locus amelogenin. After PCR amplification, the multiplex reaction is splitted into two sets of STR multiplexes by using biotin labelled primers only for one set. Using streptavidin-coated Sepharose beads five STR systems are separated from the other six systems prior to being analysed in two different runs on a capillary gel electrophoresis instrument. The multiplex system was developed and tested especially for the use in forensic casework if only limited amounts or highly degraded DNA is available, for instance, when isolated from telogen hair roots. 相似文献
272.
Tabbada KA De Ungria MC Faustino LP Athanasiadou D Stradmann-Bellinghausen B Schneider PM 《Forensic science international》2005,154(2-3):173-180
Quadruplex and pentaplex systems for polymerase chain reaction amplification of X-chromosomal short tandem repeats DXS101, HPRTB, DXS8377, DXS981 (STRX1) and DXS6789 were developed for automated profiling of liquid and membrane-bound DNA samples. Chinese, Japanese and Thai populations were typed using a quadruplex system, while German and Philippine populations were analyzed using a five-locus system. Out of 88 meioses studied in Philippine family samples at each locus, a possible one repeat deletion (allele 51 to 50) at DXS8377 was observed in a father-daughter pair. Exact tests performed on genotype data from females in the Philippine, German and Thai populations indicated that these groups conform to Hardy-Weinberg equilibrium. Exact tests for population differentiation indicate significant variations in allele distributions, particularly at loci DXS101, DXS981 and DXS6789. Considered individually, DXS8377 was the most polymorphic and HPRTB the least polymorphic locus in these five populations. When the forensic efficiency of the quadruplex system was calculated, the combined power of discrimination among males (PD(M)) was no lower than 0.998, while among females the combined PD(F) was at least 0.9999 in all populations. The combined power of paternity exclusion was a minimum of 0.998 in trio cases and 0.98 in motherless cases. The addition of locus DXS6789 to the German and Philippine population databases using a pentaplex increased the forensic efficiency of the analysis system. 相似文献
273.
A 37-year-old Turkish woman was fatally injured by her 65-year-old Turkish husband with a stab to the cervical region. After her death he inflicted a deep cut to his own neck, which he survived thanks to immediate medical assistance. To the authors' knowledge this combination of a homicidal stab to the neck and a suicidal cut to the neck has not been described before. Kosher butchering, which is common in the couple's traditional cultural environment, is discussed as possible reason for the choice of the neck as site for applying sharp violence. It may have induced the perpetrator to choose this anatomical region. 相似文献
274.
Appenzeller BM Schneider S Yegles M Maul A Wennig R 《Forensic science international》2005,155(2-3):83-90
Blood specimens from 210 drivers (179 male and 31 female) apprehended in Luxembourg from autumn 2001 to spring 2002 and requested for the determination of their blood alcohol concentration (BAC) were tested for medicinal drugs, illicit drugs, and chronic alcohol abuse (by quantification of the carbohydrate-deficient transferrin: CDT). These additional analyses were performed anonymously and with permission of state prosecutor. The 22.8% had consumed medicinal drugs, with benzodiazepines and antidepressants (10.9 and 7.6%, respectively) as main psychoactive classes. Cannabis was the most detected illicit drug (9.5%) but only one in three had THC detectable in their blood. Association of two or more psychoactive substances (poly-drug use) was observed in 27.6% of drivers (90.6% of drug consumers). On the basis of CDT values, 29.5% of drivers investigated were assumed to be chronic alcohol abusers. Statistical analysis revealed that chronic alcohol abuse and medicinal psychoactive drugs were associated with significantly higher BAC. Medicinal psychoactive drugs were clearly associated with poly-drug use, and were furthermore detected at supra-therapeutic levels in 34.9%. 相似文献
275.
The diagnosis at postmortem examination and the results of the chemical analysis in a case of a diltiazem intoxication are presented. Two metabolites were identified as desacetyldiltiazem and N-desmethyldiltiazem. 相似文献
276.
Einer der viel beschworenen Vorteile des Föderalismus ist die Flexibilität, mit der sich zentralstaatliche Anliegen den regionalen Gegebenheiten anpassen lassen. Wir untersuchen diese Vorstellung am Beispiel des Asylrechts. Ausgangspunkt ist die Überlegung, dass die Vollzugsdelegation an die Kantone zu Prinzipal-Agent-Problemen führt und die Kantonalbehörden zu Dienern dreier Herren macht – der Bundesverwaltung, dern einheimischen Bevölkerung und regionalen Interessengruppen. Quantitativ zeigt sich, dass die Restriktivität der kantonalen arbeitsmarktlichen Ausführungsbestimmungen des schweizerischen Asylgesetzes nicht primär von der Nachfrage nach Arbeitskräften, sondern vom Einfluss ausländerfeindlicher Kräfte in den Kantonen bestimmt ist. Die Praxis der Erteilung von Arbeitsbewilligungen an Asylbewerber hingegen steht in keinem Zusammenhang zur Restriktivität der Regelungen und orientiert sich speziell in der Romandie an der arbeitsmarktlichen Lage. Auch wenn sich der Einfluss von Sonderinteressengruppen nicht nachweisen lässt, tanzen die kantonalen Entscheidungsträger so zumindest auf zwei Hochzeiten. 相似文献
277.
Various attitutidinal, cognitive, emotional, and actional reactions to problems and needs of less fortunate people (unemployed, poor people in the developing countries, foreign workers in West Germany) were assessed in a questionnaire study with 865 respondents. The external validity of self-report data was established by external ratings. The focus was on emotional reactions (existential guilt, sympathy, moral outrage because of unjust disadvantages, anger about the disadvantaged, contentment with one's own advantages, fear of losing these, hopelessness with respect to the fate of the less fortunate). Several justice-related variables (beliefs, views, appraisals) as well as responsibility-related variables and social attitudes were assessed as predictors of emotions. The importance of justice-related variables for the arousal of different social emotions was clearly shown. The use and usefulness of cognitive models of discrete emotions is discussed. The impact of emotions on the readiness to various forms of prosocial activities in favor of the less fortunate was also shown: Moral outrage and existential guilt proved to be much more salient predictors than sympathy. Crucial differences between these three prosocial emotions as well as the impact of justice-related variables on readiness to prosocial activities are discussed. 相似文献
278.
Ways of Knowing and Inclusive Management Practices 总被引:1,自引:0,他引:1
Martha S. Feldman Anne M. Khademian Helen Ingram Anne S. Schneider 《Public administration review》2006,66(S1):89-99
The authors engage structural and agentic perspectives to examine opportunities for deliberation and the purposeful role of managers in creating those opportunities. Drawing on actor-network theory as a way of understanding the process of structuring knowledge, this essay focuses on the continuous enactment and reenactment of networks of human and nonhuman actants and the associations that connect them. This thinking is applied to policy issues, which the authors propose should be understood as ways of knowing. The fluidity of such ways of knowing provides opportunities for public managers to use the inclusive practices associated with boundary experiences, boundary objects, and boundary organizations to facilitate deliberation. 相似文献
279.
In the late 1990s, the Workers' Party (PT) government of the Brazilian state of Rio Grande do Sul introduced participatory budgeting, a process in which citizens establish annual investment priorities in public assemblies. This innovation was one of several attempts by incumbent parties to structure political conflict using budget institutions. The character of participatory budgeting is most evident in its policymaking processes and policy outcomes. The process circumvented legislative arenas where opponents held a majority, privileged participation by the PT's voter base, and reached into opposition strongholds. The outcomes favored the interests of potential supporters among poor and middle-class voters. The political project proved vulnerable to its own raised expectations: it failed to sustain the image of clean government; brought tax increases along with fiscal insecurity; and left unfulfilled the participants' expectations for targeted investments. This article highlights the role of participatory budgeting, indeed all budgeting, in partisan actors' institutional choices. 相似文献
280.
Christina J. Schneider 《Swiss Political Science Review》2006,12(2):67-94
Die Arbeit untersucht die Auswirkungen der Vergabe eingeschränkter Mitgliedschaftsrechte auf den Verlauf und die Ergebnisse der EU‐Osterweiterung. Die Studie zeigt auf, dass die EU‐Mitgliedstaaten Übergangsfristen für die Liberalisierung der Arbeitsmärkte durchsetzten, um die zu erwartenden Erweiterungsgewinne zugunsten der, von der Freizügigkeit negativ betroffenen Altmitglieder ‐ Deutschland, Österreich und Italien ‐ umzuverteilen und so ein Scheitern der Aufnahmeverhandlungen zu verhindern. Die Diskussionen der EU‐Mitglieder über die Einführung einer eingeschränkten Arbeitnehmerfreizügigkeit für die Neumitglieder und ein Überblick über andere Verhandlungskapitel legen nahe, dass die Verteilung differenzierter Mitgliedschaftsrechte an neue Mitglieder eine bedeutsame Handlungsalternative insbesondere zur Nichtmitgliedschaft darstellt. 相似文献