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281.
282.
The potential contribution of MVR-PCR to paternity probabilities in a case lacking a mother. 总被引:2,自引:0,他引:2
K Tamaki X L Huang M Mizutani T Yamamoto R Katsumata R Uchihi Y Katsumata A J Jeffreys 《Journal of forensic sciences》1999,44(4):863-867
Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) was applied to a paternity case lacking a mother to evaluate the paternity probability. After three flanking polymorphic sites at each of MS31A and MS32 loci were investigated from the child and alleged father, allele-specific MVR-PCR was performed using genomic DNA. It was confirmed that one allele in the child was identical to that in the alleged father at both loci. Mapped allele codes were compared with allele structures established from population surveys. No perfect matches were found although some motifs were shared with other Japanese alleles. The paternity index and probability of paternity exclusion at these two MVR loci were then estimated, establishing the power of MVR-PCR even in paternity cases lacking a mother. 相似文献
283.
Detection of anabolic steroids in head hair 总被引:1,自引:0,他引:1
We developed a gas chromatography/mass spectrometry method for detection and quantitation of anabolic steroids in head hair. Following alkaline digestion and solid-phase extraction, the MO-TMS derivatives gave a specific fragmentation pattern with EI ionization. For stanozolol, the TMS-HFBA derivative showed several diagnostic ions. For androstanolone, mestanolone (methylandrostanolone), and oxymetholone two chromatographic peaks for cis and trans isomers of derivatives were seen. Recoveries were 35 to 45% for androstanolone, oxymetholone, chlorotestosterone-acetate, dehydromethyltestosterone, dehydrotestosterone, fluoxymesterone, mestanolone, methyltestosterone, and nandrolone; 52% for mesterolone, trenbolone; 65% for bolasterone; 24% for methenolone and 17% for stanozolol. Limits of detection were 0.002 to 0.05 ng/mg and of quantitation were 0.02 to 0.1 ng/mg. Seven white male steroid abusers provided head hair samples (10 to 63 mg) and urine. In the hair samples, methyltestosterone was detected in two (confirmed in urine); nandrolone in two (also confirmed in urine); dehydromethyltestosterone in four (but not found in urine); and clenbuterol in one (but not in urine). Oxymethalone was found in urine in one, but not in the hair. One abuser had high levels of testosterone: 0.15 ng/mg hair, and 1190 ng/mL urine. We conclude that head hair analysis has considerable potential for the detection and monitoring of steroid abuse. 相似文献
284.
探寻FOS蛋白在心肌早期缺血再灌流损伤中变化规律,为心性猝死的诊断提供新方法。利用SD大鼠建立心肌缺血再灌流损伤模型,设立正常、缺血对照组与缺血再灌组。心脏标本经HE染色及免疫级化观察。结果发现,在冷冻切片上缺血20min再灌流30min,再灌流区心肌细胞核呈阳性着色。但在石蜡切片上,缺血30min再灌流30min后,再灌流区才有心肌细胞核(37.76%±9.66%)呈弱阳性着色,再灌流60min后核呈棕褐色阳性染色,120min后开始减弱(35.36%±3.16%)。正常和单纯缺血组心肌细胞核未见有阳性反应。HE染色无明显病理改变。结果提示,SABC-FOS免疫组织化学方法最早可揭示心肌缺血20min再灌流30min的损伤,FOS蛋白在再灌流后60-120min之间可能有一个高峰表达。此法对显示实验性心肌早期缺血再灌流损伤有重要的价值。有望用于心性猝死的诊断。 相似文献
285.
In order to investigate the relationship between the retrograde changes of the skeletal muscle and the time of death in various postmortem intervals (PMI), a systemic study of the enzymehistochemical activity of AChE, SDH, LDH, Ca(2+)-ATPase and the immunohistochemical reaction of SYN in motor end-plates and muscle fibers was conducted in rats under different temperatures and at various PMI. The results were analyzed and compared by an image processing system. It was found that these changes were related to the PMI, especially AChE changes. The AChE could be used as a sign-enzyme of skeletal muscle to date death. 相似文献
286.
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288.
We analyzed the postmortem blood of a total of 100 fatal cases for beta-hydroxybutyric acid (BHBA). In 25 cases of sudden and unexpected death of alcoholics we found pathologically increased levels of BHBA of 1260 to 47200 (median 8000) micromol/L. This led us to the diagnosis of an alcoholic ketoacidosis (AKA) as cause of death in these cases. The control group of 69 postmortem cases revealed that BHBA concentrations below 500 can be regarded as normal, and values up to 2500 micromol/L as elevated. Our study shows that BHBA values over 2500 micromol/L could lead to death, if no medical attention is sought. During storage we did not find any indication of postmortem formation or decomposition of BHBA in blood in vitro or in the corpses. In our opinion, BHBA should be considered the diagnostic marker of choice for the postmortem determination of alcoholic ketoacidosis (AKA) as the cause of death. The classical indications of such deaths are: unexpected death of a chronic alcoholic; none or only traces of ethanol in the blood; increased acetone blood concentration; and neither autopsy, histology, microbiology, nor toxicology reveal the cause of death. In six further cases a diabetic ketoacidosis (DKA) was diagnosed as the cause of death. 相似文献
289.
Saito T Yamamoto I Kusakabe T Huang X Yukawa N Takeichi S 《Forensic science international》2000,112(1):65-71
A 30-year-old male truck driver, known to be asthmatic, was found dead at the roadside lying near his car. A nebulizer bottle of Berotec (fenoterol hydrobromide) was found near his hand. The anatomic cause of death was suspected to be asthma. Toxicological screening of urine using Triage demonstrated the presence of methamphetamine. The blood concentration of methamphetamine was 0.4 microg/ml, and fenoterol was not detected. Hair analysis clearly indicated chronic methamphetamine abuse and medium dependency during the 2 months before death. We conclude that death might have been induced by the interaction of fenoterol and methamphetamine. 相似文献
290.
人脑挫伤后GFAP,PCNA的法医病理学研究 总被引:3,自引:0,他引:3
采用在实际工作中所遇到的脑挫伤案例标本 ,利用免疫组织化学染色 ,观察人脑挫伤后GFAP ,PCNA的改变 ,用图象分析仪对免疫组织化学染色阳性细胞的面积和灰度等进行定量测量 ,EpiInfo统计软件分析 ,得出人脑挫伤后GFAP ,PCNA变化的时间性规律 ,为脑挫伤形成时间推断 ,提供新的形态学依据。GFAP阳性细胞面积、灰度伤后24h即有显著增加 (P<0 01) ,7天达高峰 (P<0 01) ,以后有逐渐下降的趋势 ;PCNA阳性细胞面积、灰度于伤后4天达到最大值 (P<0 01) ,以后逐渐下降。因此 ,伤后人脑组织GFAP、PCNA免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,GFAP、PCNA可作为2天到20天间不同时间脑损伤的推断指标。 相似文献