罂粟种子中的鸦片生物碱及食用罂粟子后血尿中吗啡的检验

<正> 早在几百年以前已有一些关于在罂粟种子中发现吗啡的报道。然而,人们对这个问题一直争论不休。我们对几种含有罂粟种子的商品进行了分析。将这些样品用柠檬酸提取后,用高效液相色谱测定了吗啡含量。发现在每种商品中都含有相当量的鸦片生物碱。为了测定食用罂粟糕     

Age estimation of unaccompanied minors. Part I. General considerations

In recent years many countries have experienced a sharp increase in the demand for forensic age estimates of unaccompanied minors. In many countries the age thresholds of relevance to criminal prosecution lie between 16 and 22 years. In line with recommendations issued by the Study Group on Forensic Age Diagnostics, for determining the age of live subjects a forensic age estimate should combine the results of a physical examination, an X-ray of the hand and a dental examination which records dentition status and evaluates an orthopantomogram. To assess the age of persons who are assumed to be at least 18 years old, an additional radiographic or CT examination of the collar bones is recommended. Forensic age estimates should take account of the ethnic origin and socio-economic status of the person under examination.     

《道路交通事故受伤人员伤残评定》中精神伤残程度司法鉴定相关问题探讨

在法医精神病学鉴定实践中,精神伤残的评定越来越多。现行《道路交通事故受伤人员伤残评定》标准中关于精神伤残评定的相关条款过于笼统,可操作性差,常因认识不一致而产生歧义。本文结合典型案例主要从三个方面对精神伤残司法鉴定中常见问题进行探讨:(1)在评定智力缺损程度时,应结合伤前智商进行评定;(2)精神伤残评定的时间应在医疗终结后一年;(3)精神伤残评定中应引入量表进行客观评价。     

人体器官肺的体积、密度检测及其医学和法医学意义

目的积累不同种族人体器官组织体积和密度的基本数据。为病理学和法医学尸检提供简便、可靠的诊断方法。方法应用电子智能化体积-密度仪,同步检测正常人及各种疾病死者肺的质量、体积、密度。结果获得正常肺和不同程度弥漫性肺水肿病变器官体积和组织密度;在2例医源性循环超负荷和水中毒案件的法医病理学鉴定中,用肺密度值证实了常规病理组织学形态不典型的严重低渗性肺水肿。结论大体检测器官密度对尸体解剖的综合病理诊断具有一定的辅助意义。     

两种DNA提取法对不同色泽肋软骨STR分型成功率的比较

目的比较两种DNA提取法对不同色泽肋软骨的DNASTR分型结果的影响。方法利用Chelex-100法和酚-氯仿法,分别对30例不同色泽的腐败尸体肋软骨进行DNA提取,STR复合扩增,ABI3100型基因分析仪对扩增产物进行检测。结果用酚-氯仿法提取的30例腐败尸体肋软骨,均检测到全部STR基因座的等位基因型。用Chelex-100法提取的肋软骨中,22例(11例白色、8例淡黄色、3例黄色)检测出全部STR基因座的等位基因型;7例(3例黄色、4例黄褐色)检测出部分STR基因座的等位基因型;1例黑灰色的腐败尸体肋软骨,未检测出STR基因座的等位基因型。结论根据肋软骨的色泽,选择适宜的DNA提取方法。对于颜色较深的肋软骨,用酚-氯仿法进行DNA提取有助于提高其STR基因座的检出率。     

Color separation in forensic image processing

In forensic image processing, it is often important to be able to separate a feature from an interfering background or foreground, or to demonstrate colors within an image to be different from each other. In this study, a color deconvolution algorithm that could accomplish this task is described, and it is applied to color separation problems in document and fingerprint examination. Subtle color differences (sometimes invisible to the naked eye) are found to be sufficient, which is demonstrated successfully for several cases where color differences were shown to exist, or where colors were removed from the foreground or background. The software is available for free in the form of an Adobe Photoshop-compatible plug-in.     

Forensic genetic analysis of mitochondrial DNA hypervariable region I/II sequences: an expanded Korean population database

We have analyzed variation of the mitochondrial DNA (mtDNA) hypervariable segments I and II (HVS-I and HVS-II) in 185 randomly chosen individuals from Korea to provide an expanded and reliable Korean database. Combined sequence comparison of HVS-I and HVS-II led to the identification of 167 different haplotypes characterized by 154 variable sites. One hundred and fifty-one of the haplotypes were individual-specific, 14 were found in two individuals and 2 were found in three individuals. A pairwise comparison of the 185 HVS-I/II sequences found an average of 10.11 +/- 4.63 differences between individuals. The random match probability and gene diversity for the combined hypervariable regions were estimated at 0.66% and 0.9988, respectively. Analyzing the expanded database including three previously reported data sets and the present data using haplogroup-based comparisons and comparison with closely related sequences allowed errors to be detected and eliminated, thus considerably improving data quality. Sample division comparisons based on PhiST genetic distance measures revealed no significant population differentiation in the distribution of mtDNA sequence variations between the present data set and a database in The Scientific Working Group on DNA Analysis Methods (SWGDAM), but did indicate differences from other sets of data. Based on the results of mtDNA profiles, almost all of the mtDNA types studied here could be classified into subsets of haplogroups common in east Asia, and show that the Koreans possess lineages from both the southern and the northern haplogroup complexes of east Asian populations. The new data, combined with other mtDNA sequences, demonstrate how useful comparison with closely related mtDNA sequences can be for improving database quality, as well as providing haplotype information for forensic and population genetic analyses in the Korean population.