Y-chromosome STR haplotypes in three different population groups from Ecuador (South America)

POPULATIONS: Over 102 unrelated Mestizos, 102 Native Amerindian (Kichwas), and 102 African Americans who represent the three largest communities of the country Ecuador (South America).     

Skeleton find from Roman times suspected as an homicide victim

A human skeleton was found during construction work on the concrete foundations of a fence built 17 years before. The situation seemed to suggest that a corpse had been disposed of 17 years ago. Forensic and osteological examinations showed that death had been caused by semi-sharp and blunt craniocerebral trauma. However, there were morphological findings which indicated that the body had been buried in soil for at least 50 years. Several additional objects found nearby and the proximity to the Limes pointed to a Roman context. With the help of the radiocarbon method, the time of death could be dated to about 245-263 AD. A recent act of violence could hence be ruled out. According to the situation at the scene, it had to be assumed, however, that the skeleton must have been discovered already during the construction work done 17 years before and that the find had not been reported. Such behaviour is a phenomenon quite often encountered in practice.     

蒙古绵羊内源性绵羊肺腺瘤病毒全基因组序列测定与囊膜蛋白的表达分析

为了克隆内源性绵羊肺腺瘤病毒(en JSRV)全基因组序列,针对env基因分析其结构并构建原核表达质粒,并诱导囊膜蛋白表达。根据Gen Bank中提供的en JSRV DNA序列(AF152615)设计引物,采用PCR方法进行序列扩增并克隆,并对克隆结果进行测序和生物信息学分析。然后将添加了Bam HⅠ和HindⅢ酶切位点的env基因片段克隆至p ET-32a载体构建表达质粒,并将质粒转化至大肠杆菌(E.coli)BL21(DE3)中诱导囊膜蛋白表达,最后利用SDS-PAGE电泳及Western-blot检测、鉴定囊膜蛋白。结果显示,en JSRV序列长7 382 bp,其中env基因片段长1 836 bp,en JSRV与en JSRV-23的同源性为97.3%。PSORTⅡ及DNAStar等软件预测分析en JSRV囊膜蛋白主要定位于细胞质、线粒体内膜等位置,且存在多个抗原表位。在37℃条件下,0.5 mmol/L IPTG诱导转入p ET32-Env的E.coli BL21(DE3),8 h后可获得分子质量约为89 ku的以包涵体形式存在的囊膜蛋白。本试验测定了en JSRV全基因序列,并成功构建了表达囊膜蛋白的p ET32a-Env载体,为今后制备en JSRV-Env抗体奠定了试验基础,同时为囊膜蛋白生物学功能的深入探讨提供了理论参考。     

把一切献给党：兵工共青团引领青年强军报国勇争先

<正>“北京最硬核的小区,你家楼下停汽车,人家楼下停坦克……”互联网上,北京丰台一个居民小区被网友们热议着。中国军视网《军迷行天下》近期播出的《停在小区的外国装甲》,将记者拉进位于北京西南部西五环外这个看起来普普通通的居民小区。国内唯一的美国“巴顿”坦克,难得一见的英国“酋长”坦克,苏制装甲、罗马尼亚战车,70式130毫米自行火箭炮、82毫米自行火炮、苏制BRDM-2反坦克导弹发射车……一辆辆承载着历史记忆的坦克装甲车一一映入眼帘,徐徐拉开了人民军工90余年的时代画卷……     

新世纪 新发展 新形象——鞍山市市长张利藩谈城市未来发展思路

记者:张市长您好!很高兴也很感谢您在百忙之中接受我们的采访.作为国家老工业基地的鞍山市,已制定了新的城市发展战略和远景规划,这对鞍山在新世纪的发展定能起到积极的推动作用.可否请您将主要内容介绍一下?     

广西在中国-东盟交流合作中的地位和作用

20世纪90年代初以来,中国与东盟的交流合作得到了全面而迅速的发展。在与东盟交流合作中的中国各省市中,广西的地位和作用尤为突出。     

内蒙古绵羊梅迪-维斯纳病毒TaqMan实时荧光定量PCR检测方法的建立及应用

为建立一种检测内蒙古地区绵羊梅迪-维斯纳病毒(MW)的TaqMan荧光定量PCR方法,根据高度保守的gag基因序列设计特异性引物和探针,通过对反应条件和反应体系的优化,建立了快速检测MW的TaqMan实时荧光定量PCR方法.结果显示,扩增相关系数为0.999,对重组标准质粒的最低检测量为1.0×100 copies/μ...     

The Road to Reengagement and Beyond

The Shanghai Communiqué, a pathbreaking accord signed by U.S. President Richard Nixon and Chinese Premier Zhou Enlai on February 28, 1972, marks a defining moment in the history of both the United States and the People’s Republic of China;50 years on, its significance endures.     

打造品牌 展现优势

多年来，河北省唐山市古冶区委统战部紧紧围绕区委中心工作，不断探索统战工作的新思路、新方法、新途径，坚持“狠抓重点、带动一般，以活动为载体凝心聚力”的工作思路，打造了自己的统战工作品牌。