The fate of the chemical warfare agent during DNA extraction

Forensic laboratories do not have the infrastructure to process or store contaminated DNA samples that have been recovered from a crime scene contaminated with chemical or biological warfare agents. Previous research has shown that DNA profiles can be recovered from blood exposed to several chemical warfare agents after the agent has been removed. The fate of four toxic agents, sulfur mustard, sodium 2-fluoroacetate, sarin, and diazinon, in a lysis buffer used in Promega DNA IQ extraction protocol was studied to determine if extraction would render the samples safe. Two independent analytical methods were used per agent, selected from GC-MS, 1H NMR, 19F NMR, (31)P NMR, or LC-ES MS. The methods were validated before use. Determinations were carried out in a semi-quantitative way, by direct comparison to standards. Agent levels in the elution buffer were found to be below the detectable limits for mustard, sarin, sodium 2-fluoroacetate or low (<0.02 mg/mL) for diazinon. Therefore, once extracted these DNA samples could be safely processed in a forensic laboratory.     

Criminalizing Complicity: A Comparative Analysis

The Rome Statute's provision on complicity is remarkably vaguegiven the significance of the issue in international criminallaw. In search of guidance, this article takes a closer lookat the models for accomplice liability in domestic criminallaw, focusing on two systems that may be taken as representativesof the civil law and common law world, respectively: Germanand American criminal law.     

行政法的潮涨潮落

理论学派的法律是一个在认知上并不可靠的领域。它所采用的方法和视角已经受到当下政治和观念的冲击,并且因缺乏理论与实践的互动而显得摇摆不定。在本文中,我将阐述这一看似荒谬的结论,即:随着学术界与司法界不断改变对行政法形态和价值的塑造,法律将通过不断的去法化和日趋经     

To a hammer, everything looks like a nail

Legal context: Trade mark oppositions are routinely filed by brand owners againstan offending application or registration in either a pre-grantor a post-grant procedure before the respective national trademark office, but in Japan, there are several nuanced trade markopposition practice differences that should not be taken forgranted. Key issues: In light of persisting and recent abysmal trade mark opponentsuccess rates, favourable trade mark invalidation appeal (IA)data, and the inherent procedural handicaps against opponentsin Japan's current trade mark opposition system, Trade markowners would be better served by using Japan's trade mark IAprocedures to police against conflicting trade marks in Japanand not mechanistically file oppositions in Japan as one couldin other important trade mark jurisdictions like the USA orEurope. Practical significance: Japan's trade mark opposition and IA procedures offer complementaryoptions to police against conflicting trade marks so choosingthe right procedure is critical to its achieving the mission'ssuccess in curbing competitors' marks in the world's secondlargest free market economy.     

Population genetic study of eight short tandem repeat loci CSF1PO,TPOX, TH01, F13A01, FESFPS,vWA, F13B and LPL in the Western Romanian population

Allele frequencies for eight tetranucleotide short tandem repeat (STR) loci-CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, F13B, LPL-were obtained from a population sample of 105-122 unrelated individuals born in Transylvania and Banat (Romania).