Y-chromosome STR haplotypes in the Canary Islands population (Spain)

Haplotype frequencies of eight Y-chromosome STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS385) were determined from a sample of 285 unrelated males from Canary Islands.     

Developments in public affairs in Australia

• This paper discusses the integrated public affairs model most common in Australian companies, and its rationale. It describes some key characteristics of the function, drawing on a comprehensive survey of public affairs departments in late 2003. The function has become more senior and strategic, more closely aligned to business imperatives and with stable or growing budgets. It identifies areas of increased attention for practitioners, including support for organization transformation and culture change, stakeholder relations as a line, as well as staff function, reputation management and sustainability reporting.

Copyright © 2005 John Wiley & Sons, Ltd.     

Results of the 2003-2004 GEP-ISFG collaborative study on mitochondrial DNA: focus on the mtDNA profile of a mixed semen-saliva stain

We report here a review of the seventh mitochondrial DNA (mtDNA) exercise undertaken by the Spanish and Portuguese working group (GEP) of the International Society for Forensic Genetics (ISFG) corresponding to the period 2003-2004. Five reference bloodstains from five donors (M1-M5), a mixed stain of saliva and semen (M6), and a hair sample (M7) were submitted to each participating laboratory for nuclear DNA (nDNA; autosomal STR and Y-STR) and mtDNA analysis. Laboratories were asked to investigate the contributors of samples M6 and M7 among the reference donors (M1-M5). A total of 34 laboratories reported total or partial mtDNA sequence data from both, the reference bloodstains (M1-M5) and the hair sample (M7) concluding a match between mtDNA profiles of M5 and M7. Autosomal STR and Y-STR profiling was the preferred strategy to investigate the contributors of the semen/saliva mixture (M6). Nuclear DNA profiles were consistent with a mixture of saliva from the donor (female) of M4 and semen from donor M5, being the semen (XY) profile the dominant component of the mixture. Strikingly, and in contradiction to the nuclear DNA analysis, mtDNA sequencing results yield a more simple result: only the saliva contribution (M4) was detected, either after preferential lysis or after complete DNA digestion. Some labs provided with several explanations for this finding and carried out additional experiments to explain this apparent contradictory result. The results pointed to the existence of different relative amounts of nuclear and mtDNAs in saliva and semen. We conclude that this circumstance could strongly influence the interpretation of the mtDNA evidence in unbalanced mixtures and in consequence lead to false exclusions. During the GEP-ISFG annual conference a validation study was planned to progress in the interpretation of mtDNA from different mixtures.     

Service evaluation of electronic monitoring (GPS tracking) in a medium secure forensic psychiatry setting

In 2010, following a series of high-profile absconding incidents, electronic monitoring (EM) using Global Positioning System technology for patients on leave was trialled as part of a comprehensive protocol for risk management and recovery. We conducted a preliminary evaluation of effect on leave and leave violation. The total number of leave episodes and leave violations over a four-month period prior to the introduction of EM was compared with the totals in two corresponding follow-up periods in the two years after the introduction. Total episodes of leave increased by almost 60%. There was a significant association between year and type of leave episode, with leave episodes after the introduction of EM more likely to be unescorted. Episodes of leave violation reduced in each of the two follow-up periods after introduction of EM. These findings suggest potential benefits for speed of patient recovery, reduced length of stay, reduced costs and public safety.     

Humanitarian Intervention in the 19th Century: The Heyday of a Controversial Concept

This article dwells on the state of play of armed humanitarian intervention in the long 19^th century. It starts with the birth of the idea in previous centuries by jurists of the naturalist school. It then presents the behaviour of the great powers in three celebrated humanitarian cases of the 19^th century (the Greek independence struggle, the Lebanon-Syria massacres and the Bulgarian atrocities)with emphasis on their initial reluctance to intervene and their motives for intervening, as well as the role of public opinion in spurring intervention. The overall situation will be appraised with emphasis on the Christian bias and the Orientalist approach towards the Ottoman Empire. Then the views of international jurists (publicists) will be presented, followed by the attitude of major political philosophers. The overall picture that emerges in the 19^th century is one of striking relevance to today's concerns as seen by six concluding propositions.     

Peace building with the body: resonance and reflexivity in Colombia’s Legion del Afecto

ABSTRACT

Youth in Medellín, Colombia have been recognized as potential peacebuilders through initiatives for urban peace and non-violence, including the initiative Legión del Afecto. This paper explores the development of the Legión del Afecto in order to ask questions about the peace building potential of specific frames (e.g. coexistence) and specific strategies of mobilization (e.g. embodied). We describe how differences between the earlier and later years of the Legión have come to highlight tensions between affective versus market-based relationships in motivating youth. These tensions may be productive, but without serious attention to reflexivity, may also derail peacebuilding efforts.     

A Small Population Study on Friction Skin Ridges: Differences in Ridge Widths Between Latent and Inked Fingerprints

Morphological changes in the width of latent fingermark ridges occur naturally over time. This could be used to examine the aging process of latents and eventually estimate time of deposition. In a crime context, it is common practice to compare a questioned (aged) fingermark with a database of known (inked) prints. Therefore, in the absence of fresh fingermarks for aging purposes, it is of interest to reveal correlations between these two categories of fingerprints with regard to the widths of their ridges. The present study explores correlations of ridge widths between flat and rolled inked prints with latent fingermarks visualized with carbon black (CB) and titanium dioxide (TiO₂)-based powders among a small population of ten donors. Results revealed consistent differences between the ridge widths of latent and inked prints as well as flat and rolled impressions. Latent fingermarks visualized with CB and TiO₂ powders showed ridges with comparable widths.     

Intentional overdose of glargine insulin: Determination of the parent compound in postmortem blood by LC-HRMS

Insulin glargine is a long-acting insulin analog that is converted after enzymatic cleavage of the arginine pair of the β-chain into its main metabolite M1 (21^A-Gly-insulin), which is responsible for the hypoglycemic activity. In all the overdose cases described in the literature, only M1 concentrations have been reported, whereas insulin glargine was always absent or below the limit of quantitation. In this study, we present a case of suicide of a young nurse by injection of insulin glargine in which the parent molecule was found at a toxic concentration in blood. The determination and the discrimination of insulin glargine from human insulin and other synthetic analogs in the blood specimen were performed by liquid chromatography coupled to high-resolution mass spectrometry (Waters XEVO G2-XS QToF) and extraction after precipitation in the presence of bovine insulin (internal standard), with a mixture of acetonitrile/methanol +1% formic acid followed by purification on solid phase extraction cartridges C18. Glargine insulin tested highly positive in the blood with a concentration of 1.06 mg/L. Due to the difficulty in obtaining a M1 pure standard, the metabolite could not be dosed. This unique presence of the parent molecule, reported for the first time, can be explained by inter-individual variability in the rate of conversion to metabolite. Intravenous injection versus subcutaneous injection can also explain the presence of insulin glargine. Finally, the dose injected may have been so high that saturation of the proteolytic enzymes responsible for conversion to M1 should have occurred.