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A DNA microarray system for forensic SNP analysis 总被引:3,自引:0,他引:3
Forensic DNA analysis is routinely performed using polymorphic short tandem repeat (STR) markers. However, for degraded or minute DNA samples, analysis of autosomal single nucleotide polymorphisms (SNPs) in short fragments might be more successful. Furthermore, sequencing of mitochondrial DNA (mtDNA) is often performed on highly degraded or scarce samples due to the high copy number of mtDNA in each cell. Due to the increasing number of complete mtDNA genome sequences available, the limited discrimination power of an mtDNA analysis, may be increased by analysis of coding region polymorphisms in addition to the non-coding variation. Since sequence analysis of the coding region would require more material than generally present in forensic samples, an alternative SNP analysis approach is required. We have developed a one-colour microarray-based SNP detection system for limited forensic materials. The method is based on minisequencing in solution prior to hybridisation to universal tag-arrays. In a first outline of a forensic chip, a combination of 12 nuclear and 21 mitochondrial SNP markers are analysed simultaneously. The mitochondrial markers on the chip are polymorphisms within the hypervariable region as well as in the coding region. Even though the number of markers in the current system is limited, it can easily be extended to yield a greater power of discrimination. When fully developed, microarray analysis provides a promising system for efficient sensitive SNP analysis of forensic samples in the future. 相似文献
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M L Weaver B K Gan E Allen L D Baugh F Y Liao R H Liu J G Langner A S Walia L F Cook 《Forensic science international》1991,49(1):43-56
Results obtained from three commercial immunoassay kits, Abuscreen, TDx, and EMIT, commonly used for the initial test of urine cannabinoids (and metabolites) were correlated with the 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (9-THC-COOH) concentration as determined by GC/MS. Correlation coefficients obtained based on 26 (out of 1359 total sample population) highly relevant samples, are 0.601 and 0.438 for Abuscreen and TDx. Correlation coefficients obtained from a parallel study on a different set of 47 (out of 5070 total sample population) highly relevant specimens are 0.658 and 0.575 for Abuscreen and Emit. The immunoassay concentration levels, that correspond to the commonly used 15 ng/ml GC/MS cutoff value for 9-THC-COOH, as calculated from the regression equations are 82 ng/ml and 75 ng/ml for TDx and EMIT and 120 ng/ml and 72 ng/ml for Abuscreen manufactured at two different time periods. The difference of these calculated corresponding concentrations provides quantitative evidence of the reagent specificity differences. 相似文献
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During the 1960s, the US government entered into an agreementwith the UK government to construct a military facility in theChagos Archipelago. The UK government excised the Archipelagofrom the colony of Mauritius and created the British IndianOcean Territory (BIOT). Diego Garcia was transferred to theUnited States and the indigenous people of the entire Archipelagowere secretly banished. This process led to their chronic impoverishment.In the Bancoult cases, the English courts held the exile tobe unlawful and that the Chagossian people possess a publiclaw right of abode in the Chagos Islands. This article examineswhether international law can facilitate the resettlement tothe Chagos Islands via recognition of BIOT's non-self-governingstatus. It also explores the prospect of securing compensationfor the loss of ancestral lands by reference to existing andevolving international standards on indigenous rights. 相似文献
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The principal aim of our study is to contribute to the debate over whether regulation is enacted in the interest of consumers, producers, political agents, or some combination of these. We examine the legislative debates and subsequent regulatory bills surrounding road transportation regulation in Ireland in light of the three dominant theories which purport to explain the existence of regulation: the public interest theory and the so-called Chicago and Virginia theories. A unique feature of our study is an attempt to integrate specific rhetorical concepts used in the public policy debates into the theoretical analysis of regulation. One noteworthy conclusion of our study is that theories of economic processes affect the shaping and results of public policy. 相似文献
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Allen H. Barton 《Scandinavian political studies》1998,21(1):71-85
In 1948–49 the Sociology Office of the University of Oslo, under the guidance of Prof. Paul Lazarsfeld of Columbia University, initiated a series of studies on the Norwegian economic planning system. Part of this Planning Project was a national election survey in the fall of 1949, on a modified probability sample of 2600 people. The results show the paramount influence of economic class on voting, interpreting class as a combination of employer/employee relationship, property ownership, prestige-status, and income. Indicators of the homogeneity of class environment also played a role. Given the occupational structure of Norway, the Labor party had to win over part of the non-working-class population to obtain control of the government. They did this by winning about 1/3 of the white-collar vote, and about 1/4 of the farmers and fishermen. The policies of economic stabilization - control of inflation through price controls, wage controls, food subsidies, and rationing, and maintenance of full employment under conditions of "suppressed inflation" - were crucial to winning over these white-collar and farm voters. 相似文献