Modeling Temperature Variations Using Monte Carlo Simulation: Implications for Estimation of the Postmortem Interval Based on Insect Development Times*†

The association between insect development and temperature is well established. Thermal summation using accumulated degree-day measures is commonly used. However, the time at which evidence is collected is important in these estimates. The aim of this study was to provide a simulated model of the effect of temperatures on six dipteran species commonly associated with cadavers, from the death scene to the refrigerator, and finally at the time of autopsy. Temperatures measurements were sampled over a 16-month period from the external environment (external to the mortuary), within the mortuary refrigerator, and within the mortuary autopsy suite. Monte Carlo simulation using accumulated degree-days (ADD) was used to estimate the variations based on the mean and standard deviation of the temperature measurements. It was found that there was a negative correlation between the base temperature of the fly species (lowest temperature at which the flies will survive) and developmental likelihood. Species with high base temperatures (Chrysomya albiceps, Chrysomya chloropyga, and Musca domestica) were less likely to continue development in refrigerators than species with lower base temperatures (Lucilia sericata and Piophila casei). The findings of this study highlight the importance of recording temperature measurements and the period of refrigeration on PMI estimation especially when continued development occurs in spite of a period of cooling of the insect evidence.     

Differentiation of Morphologically Similar Human Head Hairs from Two Demographically Similar Individuals Using Amino Acid Ratios,

Human hair is frequently encountered as forensic evidence and can contribute valuable information to investigators. Conventional forensic hair analyses include microscopic hair comparison (MHC) and DNA analysis. However, MHC is not supported by statistics and DNA analysis cannot always be performed. Recent studies have demonstrated that evaluation of differences in the hair proteins may offer an alternate method to these analyses. In this study, an evaluation of the amino acids present in hair was investigated as an approach to differentiate morphologically indistinguishable hair samples from two demographically similar individuals. Proteins in the hair were digested using hydrochloric acid, and the resulting amino acids were derivatized with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) for analysis using gas chromatography-mass spectrometry (GC-MS). Eight derivatized amino acids were detected and quantified relative to an internal standard, L-norvaline, and used to construct twenty-eight amino acid ratios. Hair samples were collected from four areas of the head on various days over the course of one month, and no significant differences in amino acid ratios (p-value > 0.05) were observed among the areas of the head, and the ratios were consistent over the time period of this study. Additionally, fifteen of these amino acid ratios were found to be significantly different between the two individuals when compared using a two-sample t-test (p-value ≤ 0.05). These data indicate that amino acid analysis was able to differentiate two morphologically similar hair samples from different individuals and demonstrates the applicability of this method to distinguish similar hair samples when DNA analysis cannot be performed.     

A Policy Outcomes Comparison: Does SIB Market Discipline Narrow Social Rights?

Abstract

This article argues that the outcomes-based management schemes in social impact bonds (SIBs) simplify social impact and often ignore the complex ecosystem of more powerful actors that contribute to social problems. Using human ecological theory, it focuses on early care and education SIBs across the OECD to show how the focus on clients and metrics at the micro scale fails to achieve broad outcomes and may even narrow social rights. By contrast, SIBs that focus on meso- and macro-level actors have more opportunity to effect systemic change and broaden social rights. However, SIBs are rarely focused on powerful private market actors.     

Social Status and Classroom Behavior in Math and Science during Early Adolescence

Journal of Youth and Adolescence - Social status is a salient feature of the classroom peer ecology in early adolescence, yet research has not examined how it plays out within the domains of math...     

Feckless and reckless or forbearing and resourceful? Looking behind the stereotypes of HIV and AIDS in "fishing communities"

Over the last decade evidence has emerged suggesting that inmany countries fisherfolk, as an occupational group, are atgreater risk to HIV and AIDS than the general adult population.This high vulnerability has been explained in terms of the lifestylesassociated with fishing and related occupations, such as fishprocessing and trading. Fishermen have been portrayed as risktakers, their attitudes and behaviour shaped by the physicaland economic risks of the fishing lifestyle. Women in fishingcommunities, often engaged in fish processing and trading andproviding food and lodging in fishing settlements, are portrayedas being in subordinate social and economic positions and preyto sexual exploitation by cash-rich fishermen. There is a dangerin such lifestyle summaries that fisherfolk are characterizedas feckless risk takers with a reckless attitude to the chanceof contracting HIV. In this article we look at the lives ofsome men, women, and children living in a lake-side communityin Uganda severely affected by HIV and AIDS to illustrate howexisting portrayals of fisherfolk, and fishing communities,need to avoid stereotypes in order to better inform appropriatehealth sector and livelihood support measures.     

Residual history: memory and activism in modern Poland

This article examines the divide between national and local collective memory in Poland and investigates the role of “memory activists” in mediating and exploiting this divide. It narrows its focus to the ethnic cleansing of Poles by the Ukrainian Insurgent Army (UPA) from 1943 to 1944 and the forced relocation of Ukrainians in Poland, Operation Vistula, in 1947. It surveys local and national newspapers to understand competing interpretations and analyzes what incidents (e.g. protests, disputes, commemorations, reenactments, etc.) related to these events take place in local communities. It highlights the many actors, “memory activists,” and associations involved in pushing specific, often ahistorical, interpretations of these events – motivated by political gain, careerism, or personal conviction. It uses the theoretical works of Maurice Halbwachs and Karl Mannheim to effectively distinguish between local and national phenomenon and to elucidate the various nuances of collective memory.     

Exposing the War on Women: The Limits of Law and Power of Care to Address Sexual Violence in Contemporary Crime Drama

A comparative reading of two recent crime dramas, The Fall (TF) and The Bletchley Circle (BC), demonstrates the limits of law and potential of care to address violence against women. TF, a Nordic noir, moves beyond a gender-blind account of crime yet relies on a liberal, state-centric response that blunts its political critique. BC, a hybrid cozy-period-detective genre piece set in post-World War II London, offers a new perspective on violence against women in crime dramas. First, juxtaposing men’s and women’s post-war experiences, BC frames violence against women as an ongoing war in which women remain comrades-in-arms. Second, it suggests that while the state has a necessary role to play as a coordinator of spaces, skills, and citizens, state action alone cannot end violence against women. Rather, citizen engagement through an ethic of care may offer a new way to address such harms.     

AutoMate Express™ forensic DNA extraction system for the extraction of genomic DNA from biological samples

Abstract: The AutoMate Express? Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler? lysis buffer or PrepFiler BTA? lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep? column, the lysate in the sample tubes were loaded onto AutoMate Express? instrument and DNA was extracted using one of the two instrument extraction protocols. Our study showed that DNA was recovered from as little as 0.025 μL of blood. DNA extracted from casework‐type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day‐to‐day operation.     

Vicious dogs part 2: criminal thinking, callousness, and personality styles of their owners

Every year over 885,000 dog bites require serious medical attention. Based on human injury and insurance claims, six dog breeds were designated as "vicious" (Akitas, Chows, Dobermans, Pit Bulls, Rottweilers, and Wolf-mixes). This study was conducted to expand on previous research examining antisocial tendencies and personality styles of people choosing to own vicious breeds. Seven hundred and fifty-four college students completed a questionnaire assessing type of dog owned, criminal thinking, callousness, personality, alcohol usage, and deviant lifestyle behaviors. Vicious dog owners reported significantly higher criminal thinking, entitlement, sentimentality, and superoptimism tendencies. Vicious dog owners were arrested, engaged in physical fights, and used marijuana significantly more than other dog owners. However, the homogeneous sample utilized could impact the generalizability of these findings. Choosing to own a vicious dog may be a "thin slice" indicator of more antisocial tendencies.