Globalisation,civil society,and the multilateral system

This paper analyses the significance and scope of globalisation, focusing on its implications for the autonomy of national actors, on the one hand, and on the new demands that global governance imposes upon multilateral action, on the other. It is argued that the current form of globalisation is in fact compatible with some degree of autonomous coordinated social action outside the realm of the market. This allows us both to differentiate between the realities and mystification (i.e. ideology) that underlie the concept of globalisation and to reject the standard discourse and economic therapy offered by certain international organisations to developing countries. If globalisation does not rule out the possibility of autonomous nationallevel action, it also establishes the basis for more solid and effective multilateral action. The factors that support the need for such action in the future are analysed; action that responds to demands for greater management of international public assets, and to calls for more effective global governance. The article ends by identifying the essential characteristics of such a multilateral system if it is to meet the needs arising from a new international reality.     

Instability of calcium channel antagonists during sample preparation for LC-MS-MS analysis of serum samples

1,4-Dihydropyridines calcium channel antagonists (1,4-DHP CCAs) are photolabile and the products of their photodecomposition have no pharmaceutical activity. In our previous work we have presented a screening procedure for eleven 1,4-DHPs in plasma by LC-MS-MS using multiple reaction motoring. The laboratory process includes preparation and storage of stock solutions, plasma storage, solid-phase extraction, reconstitution of extracts and storage time in an autosampler for LC-MS-MS analysis. Prior to validation of the analytical procedure, we have tested the stability of these compounds by exposure to light. Methanolic solutions have been exposed to laboratory and UV light and the stability of the compounds in plasma was tested by exposure of spiked plasma samples to laboratory light at room temperature. Stability during freeze-thaw cycles and stability during 2 month storage at -20 degrees C have been tested as well. Products of photodecomposition have been identified after forced degradation and the degree of degradation has been quantified using LC-UV-DAD and LC-MS-MS, respectively. A 96% degradation after only 2h has been observed when solutions of nifedipine or nisoldipine were exposed to laboratory light in clear glass vials. In plasma samples degradation was 25% in only 2h for both compounds. The main degradation product was produced by oxidation of the dihydropyridinic ring resulting in the pyridine analogue that has been described as the first metabolite in the metabolic pathway. Only minor degradation was found for the other tested compounds after 2h light exposure in methanolic solutions. Furthermore, lercanidipine and nicardipine were also degradated by esterhydrolysis. Several additional minor degradation products were found for the other tested 1,4-DHPs, however, some of them could not be identified. Preconditions for storage and handling of plasma samples prior to and during analysis for 1,4-DHP CCAs are suggested in order to avoid photodecomposition of the analytes.