Multivariate analysis for estimating the age of a bloodstain

Our objective is to provide crime laboratories with a technique for estimating the age of a bloodstain. Toward that goal, we have used multiplexed, real-time RT-PCR (or qPCR) to determine the relative stability of different-sized segments of the same RNA species as well as differences in stability between two different RNAs' change over time in bloodstains. Our results indicate that a multivariate analysis of the changing ratio of the different RNA segments can be used to differentiate between samples of different ages in the defined population. Bloodstains from 29 of 30 donors could be partitioned into different ages using this technique. Although further improvements will be required before this approach can be implemented in crime laboratories, the multivariate analysis holds promise of providing a reliable approach for temporally linking a bloodstain to the commission of a crime or excluding a bloodstain as being irrelevant to the case in question.     

Comparison of Decomposition Rates and Faunal Colonization of Carrion in Indoor and Outdoor Environments

Abstract: Decomposition and insect colonization of pig (Sus scrofa L.) carcasses were observed over a 42‐day period inside and outside a house in a suburban region of Edmonton, Alberta. Three freshly killed pig carcasses were placed outdoors on grass and three carcasses were placed in separate rooms inside a house in a suburban residential area. The carcasses were examined and sampled regularly. Outside carcasses were colonized rapidly by Calliphora vicina (R‐D), Lucilia sericata (Meigen), Lucilia illustris (Meigen), Eucalliphora latifrons (Hough), Cynomya cadaverina (R‐D), Phormia regina (Meigen), and Protophormia terraenovae (R‐D). There was a delay of 5 days before inside carcasses were first colonized but all species except E. latifrons and L. illustris readily colonized at this time. Many more insects colonized the outside carcasses, and these were rapidly skeletonized. Inside, much fewer insects were present and decomposition was slowed and colonization extended. Dispersal patterns of postfeeding larvae inside the house on hard substrate were greatly expanded compared with those from carcasses on grass.     

Intergenerational child abuse and coping

Many studies have investigated the consequences of child sexual abuse (CSA) but few have examined the intergenerational effects of poly- victimization and maladaptive coping. The purpose of this investigation was to examine patterns of maltreatment and maladaptive coping among second-generation CSA survivors. It is hypothesized that: (a) maternal CSA history would be associated with a higher incidence of poly-victimization and maladaptive coping and (b) experiencing more forms of abuse would mediate the relation between maternal CSA history and maladaptive coping behaviors. The method used was a chart review of 139 sexually abused females aged 12 to 17, examining maternal abuse history, maladaptive coping behaviors, and child maltreatment. The results showed that poly-victimization differed as a function of maternal CSA history but maladaptive coping did not. Experiencing more types of abuse was associated with both self-injurious behaviors and substance use. In conclusion, results support the hypothesis that second generation CSA survivors are more likely to experience poly-victimization. Future research should address how intergenerational patterns of abuse might affect presenting symptomatology and treatment outcome.     

Personality and Political Discussion

Political discussion matters for a wide array of political phenomena such as attitude formation, electoral choice, other forms of participation, levels of political expertise, and tolerance. Thus far, research on the underpinnings of political discussion has focused on political, social, and contextual forces. We expand upon this existing research by examining how individual personality traits influence patterns of political discussion. Drawing on data from two surveys we investigate how personality traits influence the context in which citizens discuss politics, the nature of the relationship between individuals and their discussion partners, and the influence discussion partners have on respondents’ views. We find a number of personality effects and our results highlight the importance of accounting for individual predispositions in the study of political discussion.     

Partisan legitimacy across generations

In this paper we argue that parties shape their supporters’ views about the political system via the messages they communicate about the desirability of the political system. Moreover, we contend that the effectiveness of such communication varies considerably across generations. Combining data from election surveys collected in 15 democracies as part of the Comparative Study of Electoral Systems (CSES) project with data on the policy positions of 116 political parties collected by the Comparative Manifestos Project, we find that supporters of parties that express positive positions toward the political system report systematically higher levels of political legitimacy than supporters of parties that communicate negative views. Moreover, this communication is particularly effective among older party identifiers whose partisan identification tends to be more pronounced. Taken together, these findings suggest that political parties play an active role in shaping citizens’ views of the political system but their success in mobilizing consent among citizens in contemporary democracies may weaken with partisan de-alignment and generational change.     

Differentiating cause-of-death terminology for deaths coded as sudden infant death syndrome, accidental suffocation, and unknown cause: an investigation using US death certificates, 2003-2004

We compared written text on infant death certificates for deaths coded as sudden infant death syndrome (R95), unknown cause (R99), and accidental suffocation (W75). Using US mortality files supplemented with the death certifiers' written text for all infant deaths with International Classification of Diseases (ICD)-10 assigned codes R95, R99, and W75, we formed cause-of-death subcategories from common themes identified from the written text. Among all infant deaths in 2003-2004, the underlying cause of death was listed as R99 for 2128 deaths, R95 for 4408 deaths, and W75 for 931 deaths. Among the postneonatal deaths, the differences in subcategories varied between assigned ICD-10 codes: for R99-coded deaths, 45.8% were categorized as "Unknown" and 48.6% as "Pending"; for R95-coded deaths, 67.7% were categorized as "sudden infant death syndrome (SIDS)"; and for W75-coded deaths, 76.4% were categorized as "Suffocation." Examination of the written text on the death certificates demonstrates variability in the assigned ICD-10 codes which could have an important effect on the estimates of SIDS cases in the United States.     

Picturing neuroscience research through a human rights lens: imaging first-episode schizophrenic treatment-naive individuals

In this paper we examine imaging research involving first-episode schizophrenic treatment-naive individuals (FESTNIs) through a legal human rights lens; in particular, the lens of the Additional Protocol to the Convention on Human Rights and Biomedicine Concerning Biomedical Research. We identify a number of ethical and legal hot spots highlighted by the Protocol, and offer a series of recommendations designed to ensure the human rights compatibility of this research. Subsequently, we argue that the lack of reporting on design elements related to ethical concerns frustrates commitments at the heart of the human rights approach, namely, transparency and openness to international scrutiny. To redress this problem, we introduce two norms for the first time: ethical transparency, and ethical reproducibility. When concluding, we offer a set of reporting guidelines designed to operationalize these norms in the context of imaging research involving FESTNIs. Though we will not make this case here, we believe that parallel reporting guidelines should be incorporated into other areas of research involving human subjects.     

Temporal analysis of the cocaine metabolite benzoylecgonine in wastewater to estimate community drug use*

Abstract: Indirect estimation methods of cocaine consumption may not reflect the real extent of cocaine use. Another approach is sewage epidemiology. This direct approach is based on analysis of a stable cocaine metabolite, benzoylecgonine (BE), in wastewater. Influent to the Lubbock (Texas) Water Reclamation Plant was sampled twice a week to assess weekly variations in estimates of cocaine consumption over a 5‐month period. BE was extracted from influent wastewater samples using solid phase extraction and analyzed using gas chromatography/mass spectrometry. Measured concentrations of BE were converted to cocaine equivalents; the estimated average daily consumption of cocaine during the study period was 1152 ± 147 g. Based on BE concentrations and sewage epidemiology, higher cocaine consumption was observed on weekends compared to weekdays (p < 0.0003). This method was effective in monitoring BE in wastewater and could be used to complement survey data in estimating cocaine use at a local level.     

Bridging cross-cultural feminisms: annie besant and women's rights in England and India, 1874-1933

Abstract

Annie Besant was a Victorian radical whose outspoken views included advocacy of women's rights and opposition to British imperial policies. In her mid-forties she went to live in India. Contesting British attempts to Westernize Indian society, Besant found herself in the seemingly anomalous position of defending traditional Indian patriarchy and resisting efforts to reform the status of Indian women. Such conservatism brought on Besant criticism not only from Western liberals and Christian missionaries, but also from many Indian social reformers. When she gradually shifted her views and voiced her support for Indian women's rights, Indian nationalists condemned her as a British imperialist. The conflict between loyalty to national heritage and opposition to traditional patriarchy is one that colonized women have commonly experienced. By examining how an anti-imperialist British feminist responded to the question of women's reform in India, this paper offers another perspective on the complexities of this dilemma.