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Germ-line Gene Therapy: A New Stage of Debate 总被引:1,自引:0,他引:1
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Mandatory reporting legislation has been adopted in every state to insure the protection of children from maltreatment. These statutes have become a significant factor in the lives of mental health professionals who work with children and families. Thirty psychotherapists who had made a recent report of child maltreatment on a client in treatment were interviewed about their experiences with reporting and the consequences of reporting for therapeutic relationships. Several potentially problematic consequences and dilemmas were identified by the therapists, including immediate and long-term effects on the therapeutic alliance and course of therapy, and conflicts of interest in working with children and families. 相似文献
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H C Lee E M Pagliaro K M Berka N L Folk D T Anderson G Ruano T P Keith P Phipps G L Herrin D D Garner 《Journal of forensic sciences》1991,36(2):320-330
Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization. 相似文献
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The Institutional Basis of Secessionist Politics: Federalism and Secession in the United States 总被引:1,自引:0,他引:1
According to the literature on secession, the most importantdeterminant of secessionist sentiment is a high level of grievanceexperienced by the would-be secessionist group. However, therationale behind using secession (as opposed to another strategy)as a grievance-amelioration strategy remains almost completelyunexplained. This article contends that the institutional settingin which political conflict and grievance are experienced playsa crucial role in whether secession is conceived of as a viableoption. This contention is tested by examining the institutionalenvironment of federalism and the role it played in secessionin the United States in 1860 and 1861. The United States wasuniquely vulnerable to secessionist pressuresas are allfederal states, potentiallybecause of institutional structuresthat gave constituent states a high level of institutional authorityand capacity; this, in turn, contributed to a discourse of states'rights that included the contested right to secede. 相似文献
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Anderson L 《Time》2008,171(19):88-89
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Mary B Anderson 《Development in Practice》2000,10(3-4):495-500
Despite a growing emphasis by aid agencies on local participation and consultation, the recipients of aid commonly have mixed, if not hostile, responses to relief assistance. Agencies need to acknowledge the inequalities that are inherent in an aid relationship, and be more judicious in determining their proper role. The author calls for aid providers and recipients to accept our innate human equality and our circumstantial inequality in order to establish relationships of mutual respect and contemporaneous enjoyment of each other. 相似文献