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161.
162.
Starting from the premise that public management, in the context from the emergent knowledge society, needs new forms to act and new tools to reach its objectives, this paper aims to find evidence of the adoption of the principles of intellectual capital under the public management, by raising scientific articles about the subjects, and aims to select theoretical referential about intellectual capital and public management, by bibliometric analysis. The processes presented 100 constant articles in the final portfolio, available in the data base Web of Science, Science Direct and Scopus. The bibliometric indicators show that the studies were published as from 1969, released by 15 international periodicals, written by 164 authors, used 241 key words related to the constructs of this study. It is believed that the identification of the main references helps the understanding of the foundations of this research field. Additionally, this paper provides many inputs that contribute to the comprehension of the subject at an international level, and that can be utilised in the development of future works. 相似文献
163.
Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies 总被引:8,自引:0,他引:8
Alonso A Martín P Albarrán C García P García O de Simón LF García-Hirschfeld J Sancho M de La Rúa C Fernández-Piqueras J 《Forensic science international》2004,139(2-3):141-149
We explore different designs to estimate both nuclear and mitochondrial human DNA (mtDNA) content based on the detection of the 5' nuclease activity of the Taq DNA polymerase using fluorogenic probes and a real-time quantitative PCR detection system. Human mtDNA quantification was accomplished by monitoring the real-time progress of the PCR-amplification of two different fragment sizes (113 and 287 bp) within the hypervariable region I (HV1) of the mtDNA control region, using two fluorogenic probes to specifically determine the mtDNA copy of each fragment size category. This mtDNA real-time PCR design has been used to assess the mtDNA preservation (copy number and degradation state) of DNA samples retrieved from 500 to 1500 years old human remains that showed low copy number and highly degraded mtDNA. The quantification of nuclear DNA was achieved by real-time PCR of a segment of the X-Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y-specific fragment (AMGY: 112 bp) and a X-specific fragment (AMGX: 106 bp) making possible not only haploid or diploid DNA quantitation but also sex determination. The AMG real-time PCR design has been used to quantify a set of 57 DNA samples from 4-5 years old forensic bone remains with improved sensitivity compared with the slot-blot hybridization method. The potential utility of this technology to improve the quality of some PCR-based forensic and ancient DNA studies (microsatellite typing and mtDNA sequencing) is discussed. 相似文献
164.
Pietro Cappiello B.Sc. Francesco Zampa M.Sc. Maria Siciliano M.Sc. Biagio Amata B.Sc. Renato Di Palma B.Sc. Martino Modica B.Sc. Antonio Pasquale Lazzaro Ph.D. 《Journal of forensic sciences》2020,65(1):238-247
Thermal paper is widely used as a print medium for different applications but it constitutes a tricky substrate for fingermark visualization. An earlier work (J Forensic Sci 2015; 60 :1034) reported how to visualize fingermarks on untreated thermal paper by illuminating the item with a UV-A light source. In the present paper, the potential of the near infrared (NIR) luminescence has been tested on thermal paper compared to the mentioned method. A controlled study was carried out utilizing eccrine enriched fingermarks. The promising outcomes obtained were further confirmed by performing a pseudo-operational trial. Data clearly showed that the use of the NIR filter gave better results. Finally, preliminary tests suggested a different mechanism of reaction induced by fingermarks with respect to the one behind the thermal printing. Thus, NIR luminescence represents a refinement to the suite of optical examination processes, including the potential to increase the number of marks recovered in a noncontact, nondestructive way. 相似文献
165.
Santoro V Roca R De Donno A Fiandaca C Pinto G Tafuri S Introna F 《Forensic science international》2012,221(1-3):153.e1-153.e5
Age estimation in forensics is essential in cases involving both living and dead subjects. For living subjects, age estimation may be used to establish an individual's status as a minor in cases involving adoption, criminal responsibility, child pornography, and those seeking asylum. Criteria for age estimation in the living have recently been put forth by The Study Group on Forensic Age Diagnostics. The group has proposed guidelines with a three-step procedure: a physical examination and anthropometrical analysis; dental analysis by orthopantomogram (OPG); and X-ray study of the left hand and wrist. The board of FASE highlighted advantages and limits of each method, and suggested practical solutions concerning the age estimation process for adults and subadults. The aim of this study was to verify the applicability of the Greulich and Pyle, and Demirjian techniques on a sample group of Italians, whose ages were known, in determining the skeletal and dental age, in addition to evaluating the reliability of these techniques. 535 subjects between the ages of 7 and 15years were examined, each one undergoing both an orthopantomography (OPG) and radiography of the left wrist and hand. The data obtained underwent statistical analysis. The analyses have shown that a correlation exists between skeletal and dental age, and real age. Age estimation carried out using the Greulich and Pyle method has shown itself to be especially accurate on the Italian sample, particularly in the age ranges of 7-9years and 10.4-11.5years. The Greulich and Pyle method has shown itself to be reliable for the sample analyzed notwithstanding the ethnic differences between the original sample of reference and those analyzed in this study. Application of the Demirjian technique resulted in an overestimation of dental age. This difference is shown to be more highly significant in the higher age ranges. The combination of the Greulich and Pyle, and Demirjian methods have revealed a difference regarding real age, which turns out to be more modest in the younger age ranges and more significant in older subjects. 相似文献
166.
Alessandro di Luca M.D. Eleonora Ricci M.D. Vincenzo M. Grassi M.D. Vincenzo Arena M.D. Ph.D. Antonio Oliva M.D. Ph.D. 《Journal of forensic sciences》2019,64(1):292-294
An 18‐year‐old female patient arrived at the emergency department complaining of abdominal pain and fullness after a heavy meal. Physical examination revealed she was filthy and cover in feces, and she experienced severe abdominal distension. She died in ED and a diagnostic autopsy examination was requested. At external examination, the pathologist observed a significant dilation of the anal sphincter and suspected sexual assault, thus alerting the Judicial Authority who assigned the case to our department for a forensic autopsy. During the autopsy, we observed anal orifice expansion without signs of violence; food was found in the pleural cavity. The stomach was hyper‐distended and perforated at three different points as well as the diaphragm. The patient was suffering from anorexia nervosa with episodes of overeating followed by manual voiding of her feces from the anal cavity (thus explaining the anal dilatation). The forensic pathologists closed the case as an accidental death. 相似文献
167.
Luiz Antonio Lira M.Sc. Marcos Patrício Macedo M.Sc. José Roberto Pujol‐Luz Ph.D. Simão Dias Vasconcelos Ph.D 《Journal of forensic sciences》2019,64(3):799-804
Oxelytrum discicolle is a carrion beetle commonly found in the Neotropical region, mainly associated with cadavers and carcasses. Information on behavior related to the likelihood of cadaver colonization is scarce. We performed two field experiments in peri‐urban forest in Brazil in order to strengthen our knowledge on O. discicolle behavior. In the first experiment, we assessed the preference for piglet carcasses at different stages of decomposition offered simultaneously in two seasons, dry and rainy, while in the second experiment we investigated the diel activity of adults. A total of 493 adults were collected in the experiments. A significantly higher abundance was detected in the rainy season. There was a marked preference for carcasses at advanced stages. Adults exhibited a typical nocturnal behavior, the number trapped at night was 17 times greater than those collected during the day. Oxelytrum discicolle is nocturnal species that prefers carcasses in advanced stage of decomposition. 相似文献
168.
169.
Page Charles H. Barber Bernard Himmelstein Jerome L. Pido Antonio J. A. Kimmel Michael S. 《Society》1984,21(5):80-80
170.
FTAA Negotiations: Short Overview 总被引:1,自引:0,他引:1