Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography–tandem mass spectrometry

Zopiclone (ZOP) is a hypnotic drug prescribed to treat insomnia. Due to the chiral nature of ZOP, the psychologically active S-form and inactive R-form need to be determined enantiomerically in a forensic drug analysis. In the present study, a supercritical fluid chromatography (SFC) method was designed with a faster analysis ability than that of previously reported techniques. The SFC–tandem mass spectrometry (SFC–MS/MS) method was optimized using a column with a chiral polysaccharide stationary phase (Trefoil CEL2). ZOP was extracted from pooled human serum using solid-phase extraction (Oasis HLB) and analyzed. The developed SFC–MS/MS method achieved the baseline separation of S-ZOP and R-ZOP within 2 min. The fit-for-purpose method validation indicated that the optimized solid-phase extraction achieved near complete recovery and approximately 70% of the matrix effect. Both the retention time and peak area showed sufficient precision. The lower and upper limits of quantification (LOQ) were 5.7 × 10⁻² ng/mL and 25 ng/mL for R-ZOP, and 5.2 × 10⁻² ng/mL and 25 ng/mL for S-ZOP. The calibration line was linear in the range from lower LOQ to upper LOQ. The stability test indicated that ZOP in serum stored in a refrigerator (4°C) degraded and about 55% remained in 31 days. The quick analysis of the SFC–MS/MS method makes it a valid option for the enantiomeric analysis of ZOP.     

Shedder status—An analysis over time and assessment of various contributing factors

The shedder status of a person is an important consideration when evaluating probabilities of DNA transfer during activity-level assessments. As an extension of our previously published study, the shedder statuses of 38 individuals were reassessed 1 year later. The study found that shedder status may change over time for some individuals and was associated with one's gender, number of items touched, and mobile phone usage. In 29% of touch events, no DNA allele was detected and in 99% of touch events, the amount of DNA deposited was <2 ng. The study also found that in 0.6% of touch events, the participant could be excluded as a contributor of the observed DNA profile, with another person being included. Additionally, our investigations suggest that the current three-category system for shedder status classification may require further refinement to better represent the individuals' shedder status in a population.     

Temporary Restraining Orders in the Netherlands: A Qualitative Examination of Perpetrator and Victim Views

In 2009, the Netherlands introduced a 10-day temporary restraining order (TRO) intended for adult perpetrators of domestic violence to defuse dangerous situations and to reduce recidivism by combining a legal action with social services. For this study, 18 victims and 10 perpetrators were interviewed about their experiences with the TRO, related professional help, and the influence the TRO had had on their lives. The interviews were transcribed verbatim and subsequently qualitatively analyzed. Most of the victims were positive about the TRO period. Perpetrators were less positive. The vast majority of respondents accepted help during the TRO and were positive about the professionals’ attitudes, but not necessarily about the type of help. Most respondents reported an increase in their wellbeing, new insights, and no or less serious re-assaults by the perpetrator. The results suggest that, in the short term, TROs have a mainly positive effect on both victims and perpetrators.     

Comparing Male and Female Prison Releasees across Risk Factors and Postprison Recidivism

This study, using data from a large sample of prison releasees, examined the similarities and differences in men's and women's risk factors for recidivism involving rearrest for any crime and rearrest for a violent crime during an average 3.4-year follow-up period. Logistic regressions revealed several gender differences. Prior incarceration, time served, and specific types of prior arrest histories had differential associations with women's and men's overall and violent recidivism. Age, race, and conviction offense were gender-specific risk factors for overall recidivism, and education level and marital status were gender-specific risk factors for violent recidivism. Implications for risk assessment are discussed.     

Demographic and abuse characteristics in adolescent male sexual offenders with “special needs”

Adolescent sexual offenders with “special needs” are thought to pose different challenges for therapists working clinically. However, this population has received little attention in the literature, with the research in this area very limited. This study looked at the demographic and abuse characteristics of 24 adolescent sexual offenders with “special needs” who were compared with a group of 155 male adolescent sexual offenders with no special needs. Significant differences were found between the two groups. Those with special needs had higher levels of all forms of abuse, and differences were also found on some sub-scales of the Child Behaviour Checklist. Implications for research and clinical practice are discussed.     

Personal construct psychotherapy: Fixed-role therapy with forensic clients

Abstract

Fixed-role therapy, a dramaturgical approach to psychotherapy developed by Kelly in 1955, has been used in a variety of situations over the years with various client groups, including criminal offenders. Some of the benefits and limitations of fixed-role therapy with sexual abusers will be discussed. It is suggested that the strengths of some offenders, such as the social skills necessary to succeed at confidence games, be recognized and directed in fixed-role sessions. Illustrations of a number of issues will be drawn from two clinical case examples.     

Prevalence and blood concentrations of benzodiazepines and opioids in opioid-positive death investigations in Ontario,Canada, from 2017 to 2021

The aim of this study was to investigate the incidence of benzodiazepines in opioid-positive death investigations, including trends in frequency and combination of drugs, as well as demographic data and blood concentrations, where available. Additionally, naloxone concentrations in polysubstance compared to opioid-only cases were analyzed. This was a retrospective study that consisted of all post-mortem toxicology cases in Ontario, Canada, from January 01, 2017, to December 31, 2021, with an opioid finding in any analyzed autopsy specimen. There were 11,033 death investigations identified. The overall rate of benzodiazepine co-involvement was 54.5%. Males accounted for the majority of cases (71%), and the most affected age group was 30- to 39-year-olds. The most frequently detected opioid was fentanyl and the most frequently detected benzodiazepine was etizolam, which was also the most frequently observed opioid/benzodiazepine combination. Findings related to differences in concentrations of opioids when naloxone was also present were mostly non-significant, except for methadone. The rate of benzodiazepine detection with opioids grew faster than opioid detections overall, potentially due to the increasingly toxic drug supply. Detection of novel psychoactive drugs fluctuated more unpredictably than opioids and benzodiazepines associated with clinical use. These findings can help inform policy decisions by public health agencies in exploring harm reduction efforts, for example, education and drug-checking services.