Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.     

The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection system

This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10(-8) and 3.5 x 10(-9) mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.     

Sudden death after a cold drink: case report

We report a case of sudden cardiac death in a 12-year-old boy after rapid ingestion of a frozen slurry drink. The cause of death was determined to be a cardiac arrhythmia secondary to a previously undiagnosed cardiac rhabdomyoma with associated myocardial scarring. Ingestion of cold liquids has been associated with syncope, but not sudden cardiac death. In this case, bradycardia induced by cold-induced vasovagal reflex may have precipitated the terminal arrhythmia. Ingestion of cold liquids should be considered a potential trigger for fatal cardiac arrhythmias in patients with underlying heart disease.     

Adolescents' evaluations of Peers' motives for helping

Four groups of high-school students (N=92) were each informed about a pair of helpers, either male or female, who independently helped a sameor opposite-sex peer. Within each pair of same-sex helpers, one was presented as having helped for empathic reasons and the other was presented as having helped for nonempathic reasons. The adolescents' ratings of the helpers indicated that they generally favored empathically motivated helping in both male and female peers. However, a female helper who did not express sensitivity and emotional responsiveness to the feelings of the needy other in her prosocial moral reasoning tended to be judged more harshly by the adolescents than did a male helper who failed to express such sentiments.Received Ph. D. from Northwestern University. Research interests include empathy and prosocial behavior.All received B.S. in psychology from Kansas State University in 1987.     

B. reviews and notes: bibliographies and works of reference

WORLD SURVEY OF ISLAMIC MANUSCRIPTS. General editor: GEOFFREY ROPER. Volumes One and Two. London, Al‐Furqan Islamic Heritage Foundation, 1992–1993. 2 vols. Distributed by E.J. Brill, Leiden. Hfl. 250.‐ each vol.

MIDDLE EAST BIBLIOGRAPHY. By SANFORD R. SILVERBURG. (Scarecrow area bibliographies, no. 1.) Metuchen & London, Scarecrow Press, 1992. xxxi, 564 pp. US$69.50.

REPERTORIO BIBLIOGRÁFICO DE DERECHO ISLÁMICO (R.B.D.I.), Primera parte. By MARIA JESÚS CARNICERO, MARIBEL FIERRO, SUSANA PENA, DELFINA SERRANO, AMALIA ZOMENO‐ Madrid: Instituto de Coop‐eración con el Mundo Arabe, 1993. (Cuadernos de la Biblioteca Islámico ‘Félix María Pareja’, 55.) ix, 98pp.

THE JEWS IN IZMIR IN THE 19TH CENTURY: OTTOMAN DOCUMENTS FROM THE SHAR'I COURT. (YEHUDEY IZMIR BA‐ME'A HA‐TE?A’ ‘ESRE: TE'UDOT TURKIYYOT MIBBET HA‐DIN HA‐?AR'I). Edited by HAIM GER‐BER and JACOB BARNAI. Jerusalem, Misgav Yerushalayim, Institute for Research on the Sephardi and Oriental Jewish Heritage. 1984. vi, 114 pp.

WOMEN IN THE MUSLIM WORLD: A BIBLIOGRAPHY OF BOOKS AND ARTICLES PRIMARILY IN THE ENGLISH LANGUAGE. By HERBERT L. BOD‐MAN. Providence, Association for Middle East Women's Studies, 1991. v, 162pp. US$10.‐

NILE NOTES OF A HOWADJI: A BIBLIOGRAPHY OF TRAVELERS’ TALES FROM EGYPT, FROM THE EARLIEST TIME TO 1918. By MARTIN R. KALFATOVIC. Metuchen & London, Scarecrow Press, 1992. xxxvi, 427 pp. US$49.50.

THE WATERS OF THE NILE: AN ANNOTATED BIBLIOGRAPHY. By ROBERT O.COLLINS. London, Hans Zell, 1991. 315pp. £48.‐

DICTIONARY OF MANAGEMENT TERMS: ENGLISH‐FRENCH‐ARABIC. Compiled by JAMES COVENEY & SHEILA J. MOORE; Arabic terms edited by WAGDI RIZK GHALI & HANI G. TABRI. Beirut, Librairie du Liban, 1990. x, 109pp.

WHO'S WHO IN LEBANON 1993–1994. Twelfth edition (thoroughly revised and completed). Beirut, Publitec Publications; Munich, K.G.Saur, 1993. 482pp. £95.‐     

Coase and Bertrand on lighthouses

Coase (Journal of Low and Economics 17(2):185–213, 1974) failed to appreciate that the construction and maintenance of nineteenth-century lighthouses were in part financed by British taxpayers. Bertrand (Cambridge Journal of Economics 30:389–402, 2006) rightly calls him to account. While agreeing with Bertrand’s conclusion, we dispute her reasoning and argue that lighthouses nevertheless could have been supplied by the private sector.     

An effectiveness‐based evaluation of five state pre‐kindergarten programs

Since 1980, the number of state pre‐kindergarten (pre‐K) programs has more than doubled, with 38 states enrolling more than one million children in 2006 alone. This study evaluates how five state pre‐K programs affected children's receptive vocabulary, math, and print awareness skills. Taking advantage of states' strict enrollment policies determined by a child's date of birth, a regression‐discontinuity design was used to estimate effects in Michigan, New Jersey, Oklahoma, South Carolina, and West Virginia. For receptive vocabulary, only New Jersey and Oklahoma yielded significant standardized impacts, though two of the three other coefficients were in a direction indicating positive effects. For math, all the coefficients were positive but only Michigan and New Jersey yielded reliable results. The largest impacts were for print awareness, where all five coefficients were positive and four were reliable in Michigan, New Jersey, South Carolina, and West Virginia. The five states were not randomly selected and, on average, have higher quality program standards than non‐studied states, precluding formal extrapolation to the nation at large. However, our sample of states differed in many other ways, permitting the conclusion that state pre‐K programs can have positive effects on children's cognitive skills, though the magnitude of these effects varies by state and outcome. © 2008 by the Association for Public Policy Analysis and Management.