A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework.

A validation study was conducted to demonstrate that deoxyribonucleic acid (DNA) could be successfully extracted from human nail material and analyzed using short tandem repeat (STR) profiling and/or mitochondrial DNA (mtDNA) sequencing. This study involved the development of a DNA extraction protocol that includes a cleaning procedure designed to remove external contaminants (e.g., biological, chemical). This protocol was used to test human nail material that had been soaked in whole blood from a second donor and coated with gold-palladium to simulate scanning electron microscopic analysis. The results showed no indication of a mixture and were consistent with that of the nail donor. Fresh human nail material usually yielded both STR profiles and mtDNA sequence information; however, aged human nail material (approximately eight years old) yielded only mtDNA sequence information. Upon completion of the validation study, the extraction protocol was used for the analysis of a torn fingernail fragment recovered from the scene of a violent homicide in 1983. A partial STR profile and mtDNA sequence information indicated that the fingernail fragment was excluded as originating from the suspect and was, in fact, consistent with originating from one of the victims.     

Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid.

Prostate specific antigen (PSA, also known as p30), a glycoprotein produced by the prostatic gland and secreted into seminal plasma, is a marker used for demonstrating the presence of seminal fluid. Methods for the detection of PSA include Ouchterlony double diffusion, crossover electrophoresis, rocket immuno-electrophoresis, radial immunodiffusion, and ELISA. The extremely sensitive ELISA technique can detect PSA in concentrations as low as approximately 4 ng/mL. However, all these techniques are cumbersome and time consuming to perform in forensic laboratories, especially when only a few samples per week are processed. Various membrane tests are currently used in clinical settings to screen a patient's serum for the presence of PSA at levels greater than 4 ng/mL. In this study we evaluated three immunochromatographic PSA membrane tests by analyzing semen stains stored at room temperature for up to 30 years, post-coital vaginal swabs taken at different time after intercourse, semen-free vaginal swabs, and various female and male body fluids, including urine. The data demonstrate that PSA membrane test assays offer the same sensitivity as ELISA-based tests and provide a rapid approach for the forensic identification of seminal fluid. Furthermore, when the supernatant from a DNA extraction is used for the assay, there is essentially no DNA consumption for determining the presence of PSA in a forensic sample.     

Recovering the Indigenous Legal Traditions of India: Classical Hindu Law in Practice in Late Medieval Kerala

The collection of Malayalam records entitled Vanjeri Grandhavari, taken from the archives of an important Namputiri Brahmin family and the temple under its leadership, provides some long-awaited information regarding a wide range of legal activities in late medieval Kerala. The organization of law and the jurisprudence represented by these records bear an unmistakable similarity to legal ideas found in dharmastra texts. A thorough comparison of the records and relevant dharma texts shows that landholding Namputiri Brahmins, who possessed enormous political and economic power in the region, mediated the implementation of dharmastra into the legal system. From this comparison arise new understandings of law and legal categories such as custom and positive law. Moreover, such comparisons begin to elucidate the problems involved in Western assumptions that it is textual law, not its interpretation and application by humans, which controls behavior. The Vanjeri records demonstrate not only the importance of dharmastra as a historical document but also the manner and extent to which dharmastra provided the foundation for legal systems in Kerala as well as in other regions of India.     

The morphofunctional changes in the internal organs in the modelling of poisonings by psilocybine-containing mushrooms]

Histological analysis of the viscera in experimental poisoning with psilocybin-containing mushrooms showed nonspecific changes in all examined organs, presenting as expressed hemocirculatory disorders and intracellular dystrophy. Quantitative histochemical analysis showed appreciable shifts in the activities of enzymes involved in the cytoplasmic and mitochondrial redox processes and of specific enzymes involved in nerve tissue metabolism. This may reflect some features in the direct effects of narcotic alkaloids contained in psilocybin-producing mushrooms.     

Self-esteem and delinquency

Cross-lagged panel correlation technique is used to examine whether self-esteem has a greater effect on delinquency than delinquency has on self-esteem. Analysis of a nationwide study of tenth-grade boys shows that self-esteem is the more powerful causal factor, even when initial levels of delinquency are held constant. This result, however, is found to be stronger in the lower class than in the upper class. These data are interpreted as lending greater support to Kaplan's theory of the self-enhancing nature of delinquent behavior than to the idea of reflected appraisals.The research reported in this article was funded by a grant from the National Institute of Mental Health (MH27747), which is gratefully acknowledged. The data and tabulations utilized in this article were made available (in part) by the Institute for Social Research Social Science Archive. The data were originally collected by Dr. Jerald G. Bachman, Survey Research Center, Institute for Social Research, The University of Michigan. Neither the original collector of the data nor the archive bears any responsibility for the analyses or interpretations presented here.This article is a revised version of a paper presented at the annual meeting of the Eastern Sociological Society, Philadelphia, April 2, 1978.Received her Ph.D. from the University of Maryland and is interested particularly in the social influence on the self-concept.Received his Ph.D. from Columbia University and is interested in the structure and development of the self-concept.Received her Ph.D. from Stanford University. Current research interests are related to a longitudinal study of personality development.     

Actual and perceived intrafamilial attitudes of late adolescents and their parents

The present study assessed the comparability of adolescents' (N=184), their mothers' (N=184), and their fathers' (N=184) attitudes toward contemporary societal issues, as well as each familial group's perceptions of the other two groups' attitudes. Results of multivariated analyses of variance indicated that while there were significant overall differences between adolescents' and either parent's self-ratings for the 36 questionnaire items (dealing with such topics as drug use, sexuality, and dress codes), major (i.e., 2-scale-point) differences between generational groups existed on only about 20% of the items. However, as predicted, both adolescents and parents misperceived the extensiveness of the divisions between them. Adolescents significantly overestimated the number of major differences between themselves and their mothers and fathers, while these two parental groups significantly underestimated such divisions. These distortions in perceived attitudes were also reflected in the results of correlational analyses assessing intrafamilial attitude consistencies and inconsistencies across the 36 items. Self-alternative-family-members' perceived attitudes correlations showed greater consistency than existed in the self-alternative-family-members' actual attitudes correlations. Moreover, both analyses of variance and correlational analyses indicated that there was greater similarity between the actual attitudes of the mothers and fathers than between either parental group and their children. These results are discussed in terms of the cognitive and emotional significance of the intrafamilial attitudes of adolescents and parents.Received his Ph.D. from the City University of New York in developmental psychology. Current research interests include the relation of organismic variables to personality/social development.Received his Ph.D. in psychology from the University of Denver. Research interests include measurement theory and cognition.     

Constitutional Agreements without Constitutional Theories

How is constitution‐making possible, when people disagree on so many questions about what is good and what is right? The answer lies in the existence of incompletely theorized agreements–agreements on abstract formulations (freedom of speech, equality under the law) and on particular practices, amidst disagreement about the largest issues in social life. Such agreements help make constitutions and constitutional law possible, even within nations whose citizens cannot concur on the most fundamental matters. Incompletely theorized agreements thus help illuminate an enduring constitutional puzzle: how members of diverse societies can work together on terms of mutual respect amidst intense disagreements about both the right and the good.     

Poverty across the Life Cycle: Evidence from the PSID

The likelihood of experiencing poverty at some point during the adult life cycle is estimated. These probabilities are derived through a set of life tables built upon 25 waves of data from the Panel Study of Income Dynamics, and represent an alternative approach to studying poverty than prior empirical studies. Life table analyses are divided into early adulthood (ages 20–40), middle adulthood (ages 40–60),and later adulthood (ages 60–80). The findings indicate that individuals within the sample face a significant risk of poverty at some point during their adult lives, particularly during the early and later stages of adulthood. Duration tends to be relatively short (1 or 2 years), but once poverty occurs, it is likely to occur again. Results also reveal the profound life‐course effect that race, education, and gender have upon the likelihood of encountering poverty during the adult years. Several policy and research implications are discussed. © 2001 by the Association for Public Policy Analysis and Management.