Preparation of degraded human DNA under controlled conditions

DNA typing through analysis of short tandem repeats (STRs) and mitochondrial DNA (mtDNA) by means of the polymerase chain reaction (PCR) and sequencing are the common methods for the forensic identification of persons and reconstruction of kinship, especially when skeletal human remains have to be analyzed. Furthermore, samples typically found at crime scenes may be both quantitatively and qualitatively inadequate since they may contain very scarce and often degraded DNA due to exposure to heat, light, humidity, and microorganisms. In order to improve the performance of STR typing technology in those cases where DNA availability is limited, it would be desirable to have a source of degraded DNA with known properties. For this purpose, we have developed a method to prepare artificially degraded DNA under controlled conditions. By treatment of genomic DNA with sonication and DNAse I we have produced DNA fragments within a defined range of lengths. STR typing of this degraded DNA with a commercially available multiplex kit could only produce partial profiles as indicated by the absence of STR alleles with sizes >200 bp. This artificially degraded DNA can be used for the improvement and standardization of STR typing protocols when only highly degraded DNA is available for analysis.     

Incidence of bite marks in a selected juvenile population: a preliminary report

A study of the frequency of bite marks among sheltered children was conducted for a period of three months in the juvenile care facilities in Las Vegas, NV. The study demonstrated an incidence of 1 545 bite marks per 100 000 population. Analysis of the age, sex, and location of bite marks is presented. The study demonstrated an incidence comparable to diseases such as gonorrhea.     

Economic Burden of Child Exposure to Intimate Partner Violence in the United States

Because the effects of children’s exposure to intimate partner violence (IPV) carry long lasting consequences for the affected children, IPV exposure may impose a significant economic burden to localities, states, and society at large, made explicit over the victim’s lifetime and over a wide range of behaviors and outcomes, including use of social services, health and healthcare utilization, educational outcomes, workforce productivity, and criminal behavior. While much research has been conducted on the effect of IPV exposure on multiple short- and long-term outcomes, no research to date has examined the economic burden associated with IPV exposure. Using an incidence-based approach, we estimated the aggregate discounted costs associated with healthcare spending, criminal behavior, and labor market productivity accrued by a 20-year-old victim in 2016 projected to the age of 65, applying a 3% discount rate. The average lifetime costs derived from childhood IPV exposure are estimated to be over $50,000 per victim (2016 U.S. dollars) due to increased healthcare costs ($11,000), increased crime costs ($14,000), and productivity losses ($26,000). Over an annual birth cohort of young adults, these costs amount to over $55 billion nationwide. IPV exposure imposes a substantial economic burden to society at large in the form of increased healthcare costs, increased crime costs, and reduced productivity. This study offers an explicit quantification of substantial lifetime costs, which should encourage policy makers to redouble efforts to reduce the incidence of IPV and successfully ameliorate its effects on IPV-exposed children.     

THE REGIONALISM ALTERNATIVE IN RURAL AREAS1

This study suggests that rural reigonal councils have greater support among public officials for continuing current program activities and services than their urban counterparts. It also suggests, however, that rural regionalism may have a better chance of survival in states where regional councils have a long history of service to local governments, especially in light of present federal cutbacks. Study results contrast Georgia and Michigan regional councils.     

Greenhouse Gas Politics and Climate Change Public Nuisance Litigation

In late 2009 three long-awaited decisions were handed down in the main climate change public nuisance cases. In one case, the Northern District of California became the latest trial court to dismiss plaintiffs’ claims, while in the others the Second and Fifth Circuits reversed, allowing the plaintiffs' claims to proceed. The decisions all have been appealed. The ultimate outcome of the appeals could be affected by several legislative and administrative initiatives that are expected to see further development in 2010. This article reviews some of those initiatives and discusses their relevance to the nuisance litigation.     

New alleles and mutational events at 14 STR loci from different German populations

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.     

Aggressive,Delinquent, and Violent Outcomes of School Bullying: Do Family and Individual Factors Have a Protective Function?

Bullying perpetration at school is a long-term predictor of aggression, delinquency, and violence. Most research concentrates on risk factors for such developments, whereas protective factors have been rarely addressed. Therefore, the present study investigates potentially protective effects of family and child characteristics in a prospective longitudinal design. The sample contained 519 youngsters (253 boys and 266 girls) from the Erlangen-Nuremberg Development and Prevention Study. Bullying perpetration was measured at age 9.0 years, mothers’ and children's reports on family and individual characteristics were collected at age 10.6, and outcomes in self-reported aggressive/violent offending and mother-reported aggressive and delinquent behavior were collected at age 13.7. Hierarchical regression analyses revealed main effects and interactions. The latter suggest buffering protective effects of consistent discipline in parenting and children's intelligence, positive attitude to school, prosocial behavior skills, low hyperactivity/inattention, and an anxious/depressive mood. The findings varied across the two outcome measures and with regard to gender. Implications for further research and prevention practice are discussed.     

Research Foundations of Greenbook Interventions to Address the Co‐Occurrence of Child Maltreatment and Adult Domestic Violence

Twenty years ago the National Council of Juvenile and Family Court Judges published Effective Intervention in Domestic Violence and Child Maltreatment: Guidelines for Policy and Practice, which became known as the Greenbook because of its green cover. This article presents the social science research leading up to the Greenbook and the 20 years beyond the publication of this seminal document, including examination of the effect of co‐occurrence of child maltreatment and exposure to domestic violence on child outcomes, the interventions for families to improve the safety of both child and adult victims, and future directions for research.