Temperature‐Dependent Postmortem Changes in Human Cardiac Troponin‐T (cTnT): An Approach in Estimation of Time Since Death

Estimation of time of death is an indispensible requirement of every medico‐legal autopsy, but unfortunately, there is not a single method by which it could be determined accurately. This study focused on the temperature‐dependent postmortem degradation of cardiac troponin‐T and its association with postmortem interval (PMI) in human. The analysis involved extraction of the protein, separation by denaturing gel electrophoresis (SDS‐PAGE), and visualization by Western blot using cTnT‐specific monoclonal antibodies. The area of the bands within a lane was quantified by scanning and digitizing the image using Gel Doc (Universal Hood). The results indicate a characteristic banding pattern among human cadavers (n = 6) and a pseudo‐linear relationship between percentage of cTnT degradation and the log of the time since death (r > 0.95), which can be used to estimate the postmortem interval. The data presented demonstrate that this technique can provide an extended time range during which PMI can be more accurately estimated.     

The Reliability of Pattern Classification in Bloodstain Pattern Analysis,Part 1: Bloodstain Patterns on Rigid Non‐absorbent Surfaces

This study was designed to produce the first baseline measure of reliability in bloodstain pattern classification. A panel of experienced bloodstain pattern analysts examined over 400 spatter patterns on three rigid non‐absorbent surfaces. The patterns varied in spatter type and extent. A case summary accompanied each pattern that either contained neutral information, information to suggest the correct pattern (i.e., was positively biasing), or information to suggest an incorrect pattern (i.e., was negatively biasing). Across the variables under examination, 13% of classifications were erroneous. Generally speaking, where the pattern was more difficult to recognize (e.g., limited staining extent or a patterned substrate), analysts became more conservative in their judgment, opting to be inconclusive. Incorrect classifications increased as a function of the negatively biasing contextual information. The implications of the findings for practice are discussed.     

Fluorescence Imaging of Posterior Spiracles from Second and Third Instars of Forensically Important Chrysomya rufifacies (Diptera: Calliphoridae),

Entomological protocols for aging blowfly (Diptera: Calliphoridae) larvae to estimate the time of colonization (TOC) are commonly used to assist in death investigations. While the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. This pilot study sought to improve resolution of larval stage in the forensically important blowfly Chrysomya rufifacies using high‐content fluorescence microscopy and biochemical measures of developmental marker proteins. We established fixation and mounting protocols, defined a set of measurable morphometric criteria and captured developmental transitions of 2nd instar to 3rd instar using both fluorescence microscopy and anti‐ecdysone receptor Western blot analysis. The data show that these instars can be distinguished on the basis of robust, nonbleaching, autofluorescence of larval posterior spiracles. High‐content imaging techniques using confocal microscopy, combined with morphometric and biochemical techniques, may therefore aid forensic entomologists in estimating TOC.     

Ricochet Behavior on Glass—Critical Ricochet Angles,Ricochet Angles,and Deflection Angles

For shooting scene reconstruction purposes, knowledge about the ricochet behavior of bullets provides valuable information. In this study, the critical ricochet angles of four cartridge types were established on plain float glass. The estimates of the critical ricochet angles varied between cartridge types and were 21.0° for .32 Auto FMJ bullets, 15.8° for 9 mm Luger FMJ bullets, 17.6° for .45 Auto FMJ bullets, and 21.3° for 9 mm Luger, Action NP bullets. The corresponding ricochet and deflection angles per incidence angle varied depending on the state of the ricocheted bullets. The mean ricochet angles are always lower than the corresponding angles of incidence, and the mean ricochet angles for the FMJ bullets with undamaged jackets are lower than those of bullets where the jacket is either damaged or the bullet partially ricocheted and partially perforated. Mean ricochet angles are lower for undamaged FMJ bullets than for undamaged Action NP bullets.     

Expanding the Boundaries of Criminal Justice: Emphasizing the “S” in the Criminal Justice Sciences through Interdisciplinary Efforts

At its very core, the phrase “criminal justice sciences” points to the interdisciplinary nature of our field. However, efforts to promote and support interdisciplinary teaching and research face significant barriers. In this paper, I address how interdisciplinarity is conceptualized, the history of criminal justice as an interdisciplinary field, why we should promote interdisicplinarity, barriers we confront in interdisciplinary efforts, the degree to which ACJS members engage in interdisciplinary practices, factors related to interdisciplinarity, and opportunities for increased interdisciplinary research in criminal justice. To grow as an academic field, I conclude that we must look to other disciplines such as biology, computer science, policy, and women’s studies as we expand our disciplinary boundaries.     

Perceptions of Law Enforcement Officers in Seeking Mental Health Treatment in a Right-to-Work State

The high stress environment of law enforcement places officers at risk for a variety of mental and physical health problems; however, officers are reluctant to seek out treatment. The purpose of this study was to identify which factors associated with law enforcement officers had predictive value in the level of stigma perceived in seeking mental health treatment by currently employed, certified peace officers in Arizona. The factors included sex, age, race/ethnicity, years employed as an officer, size of the department, current rank/position within the department, the type of government operating the agency, and payment of union dues. The study included 454 participants. Participation included the completion of demographic data, the Self-Stigma of Seeking Psychological Help (SSOSH), and the Perceptions of Stigmatization by Others for Seeking Help (PSOSH). Using standard multiple regression, the most significant finding was the relationship between the size of the department and levels of stigma (p = .014); such that, the size was inversely related to the levels of perceived stigma. As the agency size increased, the perceptions of stigma decreased. Such a finding has several implications for law enforcement agencies related to preparedness and training.     

Sexual Harassment Litigation: a Road to Re-victimization or Recovery?

Various aspects of the judicial process have been hypothesized as damaging to sexual harassment plaintiffs, though limited research has been conducted that actually examines this hypothesis. We examined data from a large sample of women who participated in a class action lawsuit alleging workplace sexual harassment and discrimination (n?=?1218) and another sample of similarly situated women who opted out of litigation (n?=?465, non-litigants). We then followed the litigants for 5 years. This study takes an initial look at some of the variables theorized to play a role in the psychological outcomes of both harassment and subsequent litigation. Both the severity of harassment and participation/persistence in the litigation process were related to psychological outcomes at each of three assessments across a 5-year period; the frequency and severity of harassment, as well as plaintiffs’ cognitive appraisals of their situation, appeared to have the strongest relationship to psychological harm. Results of multivariate analysis of covariance (MANCOVA) revealed that participation and persistence in litigation played a consistent role in psychological outcomes across time, over and above the impact of harassment itself. However, litigation did not appear to be the cause of psychological outcomes as posttraumatic stress disorder (PTSD) symptomatology, in particular, was the result of the original harassment experience.     

Local demands and state policies: general councils (Meclis-i Umumi) in the Edirne and Ankara provinces (1867–1872)

By comparing the decisions of various meetings of the General Councils of Edirne and Ankara provinces from 1283 to 1288 (1867–1872 AD), this study analyses social and economic dynamics of both provinces, the state vocabulary for handling local demands, and the boundaries of responsibility for the state and the local actors in provincial administration. Instead of reading the Tanzimat as a top-down imposition, this article defines General Councils as sites of negotiations between state and local actors and instruments for local development. This article challenges the conventional view of provincial councils as weak and unable to implement various policies. It contributes to a new generation of studies that challenges the separation between state and social forces and looks at how both interacted in provincial administration.