Archaeology, science and forensic anthropology: a tribute to Dr T. Dale Stewart

The name, T. Dale Stewart is synonymous with physical anthropology. To many members of the physical anthropology section, particularly those born in the latter half of this century, he is perhaps best known for his 1979 Essentials of Forensic Anthropology. Without a doubt, much of the foundation of this discipline rests upon his teachings and influence. Few knew him in the capacity that William M. Bass did, as T. Dale Stewart was a member of Bass doctoral committee. Bass was greatly influenced during the time he spent working with Dr. Stewart in the 1950's and the instruction and guidance Stewart instilled in Bass has and will continue to be passed on to subsequent generations. Research was Dale Stewart's main emphasis and he succeeded in demonstrating the value of investigation and how results were crucial in explaining many of the processes manifest on skeletal material. Clearly his hypothesis-based approach became essential to skeletal biology and numerous procedures and methods employed in the field are synonymous with the teachings of Dr. T. Dale Stewart. By reflecting on several recently completed interdisciplinary research projects, the far-reaching impact of his knowledge and instruction can be demonstrated.     

Qualifying the psychiatrist as a lay witness: a reaction to the American Psychiatric Association petition in Barefoot v. Estelle

Organized psychiatry has recently begun to define limits to expert testimony. The American Psychiatric Association filed an amicus brief in the case of Barefoot v. Estelle urging legal curtailment of psychiatric testimony as to future dangerousness and prohibition on Constitutional grounds of expert psychiatric testimony solely based on hypothetical data. The Supreme Court refused relief on both questions. Psychiatric testimony to ultimate questions at law is limited by the inherent contextual variables of psychiatric clinical and experimental knowledge and practice. A forensic science model for psychiatric participation with explicit psychiatrically defined limitations is proposed using competence to stand trial as an example.     

Body Morph: Feasibility Testing of an Interactive CD-ROM to Teach Young Adolescents about Puberty

The aim of this pilot study was to examine the feasibility of an interactive computer program among 34 sixth and seventh grade children and to assess the potential for knowledge acquisition about puberty. Based on a developmental self-esteem approach to teach children about their bodies, the Body Morph program was designed to maximize the educational experience by: (1) tailoring puberty information to boys or girls, and (2) creating a confidential experience. Preliminary results indicated that students significantly improved their knowledge about puberty after using the program. Satisfaction among boys and girls for this type of educational approach suggests that it is a compelling format from which to learn about puberty.This research project was funded with the support of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) 1R43DK061269-01A2 to develop an educational computer program to improve body image in middle schoolers.     

The Persian Gulf War's impact on Americans' political information

Does war increase Americans' normally low levels of political information? Data from the Center for Political Studies' Political Consequences of War Study, which plumbed Americans' information about the Persian Gulf War, suggest it does, albeit slightly. Criticisms of mass public competence to influence foreign policy lose some of their sting, but there remain grounds for concern about the quality of public opinion. Old knowledge aids in the acquisition of new, and most people were too uninformed to benefit much from intensive media coverage of the Gulf crisis.     

A comprehensive ATR-FTIR spectroscopic analysis for the identification and differentiation of lip balms

Lip balm may be encountered as physical evidence in cases involving sexual assaults, homicides, and kidnappings. Lip balm can be used as corroborative evidence by providing a potential link between the victim, accused, and the crime scene. For lip balms to be used as evidence, it is important to understand the diversity and their aging process under different conditions. Therefore, in this study, ATR-FTIR spectroscopy in conjunction with chemometric tools such as principal component analysis (PCA) and linear discriminant analysis (LDA) has been used for the objective identification and differentiation of 20 brands of lip balms. Moreover, lip balms on different substrates and wearing effects over time were also investigated. The results show that the PCA-LDA training accuracy was 92.5%, whereas the validation accuracy comes out to be 83.33%. A blind study using pristine samples was also performed which resulted in 80% PCA-LDA accuracy. PCA-LDA prediction of samples on various substrates showed a higher chemometric prediction accuracy for nonporous substrates (glass, plastic, and steel), than for porous substrates (cotton cloth, cotton swab stick, dry tissue paper, and white paper) for samples kept in room temperature and under sunlight for 15 days. The substrate study showed that the samples from various substrates could effectively generate respective spectra which can help in brand-level identification even after several days. The present method demonstrates a potential for lip balm samples to be used in forensic casework applications.     

The role of DNA in criminal indictments in Israel

In their investigations of criminal cases, law enforcement agencies rely heavily on forensic evidence. Numerous studies have examined the scientific and technological advancements of DNA testing, but little evidence exists on how the availability of DNA evidence influences prosecutors' decisions to move cases forward in the criminal justice system. We created a new database by juxtaposing data from the Forensics Division of the Israel Police, which recorded the presence (or not) of DNA profiles in criminal cases (n = 9862), and data on the indictment decision for each case (2008–2019). Rates of indictments are computed for each case, and trend lines are used to present variations in the rates of indictment decisions with and without DNA profiles. Approximately 15% of all criminal cases without DNA presented to the prosecutor's office are subsequently prosecuted, compared with nearly 55% of cases with DNA profiles. The presence of DNA evidence influences the prosecutor's decision to move a case forward in the criminal justice system. Utilizing a scientific approach to prosecute offenders is a welcome development; however, DNA evidence is not infallible, and caution must be exercised in regard to DNA's overuse in the legal system.     

A systematic approach to improve downstream single-cell analysis for the DEPArray™ technology

Most commercially available STR amplification kits have never been fully validated for low template DNA analysis, highlighting the need for testing different PCR kits and conditions for improving single-cell profiling. Here, current strategies rely mainly on adjusting PCR cycle number and analytical threshold settings, with a strong preference for using 30 amplification cycles and thresholds at 30–150 RFU for allele detection. This study aimed to (1) determine appropriate conditions for obtaining informative profiles utilizing a dilution series, and (2) test the outcome on single cells using the DEPArray™ technology. Four routinely applied forensic STR kits were compared by using three different amplification volumes and DNA dilutions down to 3.0 pg, while two well-performing kits were used for single/pooled leucocyte and sperm cell genotyping. Besides reduced costs, the results demonstrate that a 50%–75% PCR volume reduction was beneficial for peak height evaluation. However, this was counteracted by an increased artifact generation in diluted DNA volumes. Regarding profile completeness, the advantage of volume reduction was only prominent in samples processed with Fusion 6C. For single and pooled cells, ESIFast and NGMDetect provided a solid basis for consensus profiling regarding locus failure, although locus dropouts were generally observed as stochastic events. Amplification volume of 12.5 μL was confirmed as appropriate in terms of peak heights and stutter frequencies, with increased stutter peaks being the main artifact in single-cell profiles. Limitations associated with these analyses are discussed, providing a solid foundation for further studies on low template DNA.