全文获取类型
收费全文 | 63篇 |
免费 | 2篇 |
专业分类
各国政治 | 5篇 |
工人农民 | 1篇 |
世界政治 | 2篇 |
外交国际关系 | 4篇 |
法律 | 44篇 |
中国政治 | 1篇 |
政治理论 | 7篇 |
综合类 | 1篇 |
出版年
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2013年 | 9篇 |
2012年 | 2篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 3篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1971年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有65条查询结果,搜索用时 11 毫秒
21.
SNPs and MALDI-TOF MS: tools for DNA typing in forensic paternity testing and anthropology 总被引:3,自引:0,他引:3
DNA markers used for individual identification in forensic sciences are based on repeat sequences in nuclear DNA and the mitochondrial DNA hypervariable regions 1 and 2. An alternative to these markers is the use of single nucleotide polymorphisms (SNPs). These have a particular advantage in the analysis of degraded or poor samples, which are often all that is available in forensics or anthropology. In order to study the potential of SNP analysis in these fields, 41 SNPs were selected on the basis of following criteria: conservation, lack of phenotypic expression, and frequency of occurrence in populations. Thirty-six autosomal SNPs were used for genotyping 21 inclusionary and 3 exclusionary paternity cases. The behavior of 5 X-chromosome SNPs was analyzed in a French representative population. Our approach to SNP typing is a multiplex PCR based amplification followed by simultaneous detection by primer extension (PEX) analyzed by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The selected autosomal SNPs showed independent inheritance and gave clear results in paternity investigation. All X-SNPs were useful as both paternity and identification markers. PEX and MALDI-TOF MS, with their high sensitivity, precision and speed, gave a powerful method for forensic and anthropological exploitation of biallelic markers. 相似文献
22.
STR-genotyping from human medieval tooth and bone samples 总被引:3,自引:0,他引:3
We extracted the DNA contained in samples of bones and teeth from 10 skeletons excavated from the Gravette site (400-1000 AD, south of France). Ancient DNA was analysed by autosomal short tandem repeats (STRs). The DNA present in these ancient remains appeared very degraded, but nevertheless, better conserved in tooth than in bone samples. Moreover, we showed that the DNA extracted from ancient dental pulp was not exempt from polymerase chain reaction (PCR) inhibitors, which could result from extreme DNA fragmentation. An adapted protocol with a supplementary step of purification removed this inhibition. 相似文献
23.
This article looks at the promise of technology to revolutionise humanitarian action, especially in terms of the gathering and use of data. With many heralding a ‘data revolution’, the opportunities and enthusiasm for using social media and SMS data in crisis response are on the rise. The article constructs an analytical framework in order to scrutinise the three main claims made on behalf of technologically advanced humanitarian information systems: that they can access data more accurately, more quickly, and alter power relations in emancipatory ways. It does so in relation to two aspects of digital humanitarianism: visual technology and crisis mapping, and big data. The article is partly informed by a historical perspective, but also by interview and other material that suggests some of the claims made on behalf of technology are exaggerated. In particular, we argue that the enthusiasm for the data is vastly outstripped by the capacity to meaningfully analyse it. We conclude by scoping the implications of the future technological evolution of humanitarianism, in particular by examining how technology contributes to what Duffield terms ‘post-modern humanitarianism’. 相似文献
24.
In cases of stains that contain mixed DNA from different contributors, analyzing mitochondrial DNA (mtDNA) requires the use of cloning techniques. We developed an efficient cloning technique that was applied in a rape case. After a differential lysis-based DNA extraction from vaginal swabs, hypervariable region I and II (HVI, HVII) amplicons obtained from the male fraction were cloned. Although we mainly found the victim's haplotype, we were able to detect the suspect's haplotype in two clones for HVI and in one clone for HVII. As the midpiece of the flagellum, which contains mitochondria, can be lost during the differential lysis, we also investigated the female fraction by cloning to evaluate the proportion of victim/suspect mtDNA. Unfortunately, only clones presenting the victim's haplotype were found. This case highlights the need for an optimal differential lysis protocol to enrich the male fraction not only with nuclear but also mitochondrial DNA. 相似文献
25.
X-chromosomal short tandem repeats (X-STRs) are very useful in complex paternity cases because they are inherited by male and female offspring in different ways. They complement autosomal STRs (as-STRs) allowing higher paternity probabilities to be attained. These probabilities are expressed in a likelihood ratio (LR). The formulae needed to calculate LR depend on the genotype combinations of suspected pedigrees. LR can also be obtained by the use of Bayesian networks (BNs). These are graphical representations of real situations that can be used to easily calculate complex probabilities. In the present work, two BNs are presented, which are designed to derive LRs for half-sisters/half-sisters and mother/daughter/paternal grandmother relationships. These networks were validated against known formulae and show themselves to be useful in other suspect pedigree situations than those for which they were developed. The BNs were applied in two paternity cases. The application of the mother/daughter/paternal grandmother BN highlighted the complementary value of X-STRs to as-STRs. The same case evaluated without the mother underlined that missing information tends to be conservative if the alleged father is the biological father and otherwise nonconservative. The half-sisters case shows a limitation of statistical interpretations in regard to high allelic frequencies. 相似文献
26.
Audrey Farrugia M.D. Jean‐sébastien Raul M.D. Ph.D. Annie Géraut M.D. Bertrand Ludes M.D. Ph.D. 《Journal of forensic sciences》2010,55(5):1371-1374
Abstract: Ricochet of a bullet in the spinal canal is well known by neurosurgeons but relatively not a common event in usual medico‐legal autopsy practice. This article presents a homicide case of a penetrating gunshot injury of the lumbar spine through the T12‐L1 intervertebral foramen with active movement of the projectile within the spinal canal to the L5‐S1 level. This case illustrates a bullet intradural and intramedullary active movement because of a ricochet of the body of T12 with active redirection of the path. In the current literature, different types of migration in caudal or cranial direction, intradural, or intramedullary are reported. If spontaneous migration of T10 to S1 seems to be more frequent, some authors reported a C1 to S2 migration. Such migration could be asymptomatic or induce neurological impairment. The medico‐legal consequences of these migrations within the spinal canal are described. 相似文献
27.
Bertrand Badie 《Global Society》2018,32(2):139-148
This article argues that the debate on “new wars” and “post-Westphalian” wars and conflicts misses a crucial dimension, that of the importance of weakness in the relations between states as well as between states and non-state entities. Most analyses of war examine power relationships between states as if power were an essential determinant of success or failure in war. Recent wars, however, show that weakness can in fact become a strength, that inequality is not so much a problem as an advantage. The author suggests the need to rename and re-problematise “new wars” as “new international conflicts” (NICs), for otherwise we miss the fundamental reasons why “powers” are often defeated, or at least held at bay by the “weakness” of their adversaries. Suggestions will also be made about how to potentially resolve such unequal conflicts and wars. 相似文献
28.
Detection and quantification of the age-related point mutation A189G in the human mitochondrial DNA 总被引:2,自引:0,他引:2
Thèves C Keyser-Tracqui C Crubézy E Salles JP Ludes B Telmon N 《Journal of forensic sciences》2006,51(4):865-873
Mutation analysis in the mitochondrial DNA (mtDNA) control region is widely used in population genetic studies as well as in forensic medicine. Among the difficulties linked to the mtDNA analysis, one can find the detection of heteroplasmy, which can be inherited or somatic. Recently, age-related point mutation A189G was described in mtDNA and shown to accumulate with age in muscles. We carried out the detection of this 189 heteroplasmic point mutation using three technologies: automated DNA sequencing, Southern blot hybridization using a digoxigenin-labeled oligonucleotide probe, and peptide nucleic acid (PNA)/real-time PCR combined method on different biological samples. Our results give additional information on the increase in mutation frequency with age in muscle tissue and revealed that the PNA/real-time PCR is a largely more sensitive method than DNA sequencing for heteroplasmy detection. These investigations could be of interest in the detection and interpretation of mtDNA heteroplasmy in anthropological and forensic studies. 相似文献
29.
Unification of legal rules in Europe is not a new phenomenon. However, nowadays, Europe is still an area with many different
jurisdictions. This paper studies the process of unification of legal rules in the European Union within a non-cooperative
game-theoretical framework. This paper contributes to the understanding of the process by concentrating on the role of the
European Commission.
In the law-and-economics literature, it is argued that national legal rules will converge more or less spontaneously through
the works of legislators and judges. But legal convergence in the European Union is not inevitable: preferences toward legal
rules differ across nation-states; substituting a legal system for another one is costly; a coordination problem may arise.
We first study the interactions of two nation-states who choose non-cooperatively their legal rules. We shall argue that the
action of the Commission is, at first sight, likely to eliminate the coordination problem (under certain conditions). Two
factors are at work. First, the Commission has a certain expertise which enables it to propose new and perhaps more efficient
rules (so that the choice of unification does not reduce to select a particular nation-state legal system). Second, the Commission
may use a system of fines that induces nation-states to abide by its rules (once these rules are adopted by nation-states).
Next, we refine our first model: the process of legal unification is viewed as a game where nation-states choose the game
that they will play. They choose if they will try to reach an agreement without resorting to the actions of the Commission,
or if they will play the game implicitely proposed by the Commission. This captures more precisely the action of the Commission,
its ‘right of initiative’’, the publication of proposals in ‘green’’ or ‘white papers’’. In this second model, a coordination
problem may arise.
JEL Classification C72 · K00 相似文献
30.