Simultaneous Detection of Human Mitochondrial DNA and Nuclear‐Inserted Mitochondrial‐origin Sequences (NumtS) using Forensic mtDNA Amplification Strategies and Pyrosequencing Technology

Next‐generation sequencing technologies enable the identification of minor mitochondrial DNA variants with higher sensitivity than Sanger methods, allowing for enhanced identification of minor variants. In this study, mixtures of human mtDNA control region amplicons were subjected to pyrosequencing to determine the detection threshold of the Roche GS Junior^® instrument (Roche Applied Science, Indianapolis, IN). In addition to expected variants, a set of reproducible variants was consistently found in reads from one particular amplicon. A BLASTn search of the variant sequence revealed identity to a segment of a 611‐bp nuclear insertion of the mitochondrial control region (NumtS) spanning the primer‐binding sites of this amplicon (Nature 1995;378:489). Primers (Hum Genet 2012;131:757; Hum Biol 1996;68:847) flanking the insertion were used to confirm the presence or absence of the NumtS in buccal DNA extracts from twenty donors. These results further our understanding of human mtDNA variation and are expected to have a positive impact on the interpretation of mtDNA profiles using deep‐sequencing methods in casework.     

Reviews

Enrique Leff: Ecologia y Capital: Hacia una perspectiva ambiental del desarrollo (Ecology and Capital: Toward an Environmentalist Perspective on Development). Universidad Autonoma de Mexico, Mexico City, 1986?

Martin Ryle: Ecology and Socialism. Century Hutchinson, London, 1988.

Klaus Eder: Die Vergesellschaftung der Natur: Studien zur sozialen Evolution der praktischen Vernunft (The Socialization of Nature: Studies on the Social Evolution of Practical Reason). Suhrkamp Verlag, Frankfurt, 1988.     

East China Sea or South China Sea,they are all China's Seas: comparing nationalism among China's maritime irredentist claims

Much has been made over the past few years of China'vs ambitions of regaining control of its irredentist claims in the East and South China Seas. While some of this speculation focuses on the massive amounts of money the People's Republic of China (PRC) has funneled into its naval modernization program, other analysts are more interested in the drivers behind the increasingly popular sentiment that the country must “reclaim” its lost territories. The Chinese Communist Party can ill afford to ignore the voice of an already disenchanted population if it hopes to stay in power, particularly in regard to matters of national pride. As a result, in dealing with China's irredentist claims, nationalism in particular can be a powerful ideological factor in shaping the nation's foreign policies. This is especially apparent in the case of irredentism, where nationalism can often override diplomatic and strategic imperatives. This paper addresses the question of how does the nationalist discourse vary between two territorial disputes, the East and South China Seas. It uses discourse analysis to examine developing trends among online social media and news sites. This in turn allows for the construction of a framework of how nationalism develops among both elite and grassroots audiences.     

Perceiving and Communicating Environmental Contamination and Change: Towards a Green Cultural Criminology with Images

In this article, we will respond to recent calls for a ‘green cultural criminology’ by attempting to open the way for new visual explorations of environmental harms and crimes, and by suggesting some methodological perspectives that can be advanced by the use and analysis of the photographic image. To demonstrate the power, potential and possibility—as well as some potential limitations—of a green cultural criminology with images, we draw on two ethnographic studies carried out in Huelva (Spain) and central Appalachia (United States). The described methods have the advantage of (1) bringing together the multiple and complex experiences of those who live in polluted areas; (2) considering the cultural meaning given to experiences of ecological change and destruction; and (3) exploring how those experiences are represented, communicated and understood. We conclude with a call to use qualitative visual approaches for carrying out research in an emergent green cultural criminology designed to develop a complex understanding of the multiple forms of environmental harms and crimes.     

Developing Public Safety and Crime Indicators in Taiwan

Many countries and international organisations (for example, the USA, England and Wales, Japan, the European Union and the Organisation for Economic Cooperation and Development) have developed measures of public safety and measures of the effectiveness of criminal justice agencies. This paper briefly considers the background to such comparative developments and relates these to the specific contextual conditions of Taiwan. We report the results of a study which reviewed the state of empirical indicator availability in Taiwan and sought to develop an indicator framework for those charged with the governmental task of ‘public safety’. The paper concludes by considering how such a framework can be implemented in Taiwan.