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71.
72.
Gusmão L Butler JM Carracedo A Gill P Kayser M Mayr WR Morling N Prinz M Roewer L Tyler-Smith C Schneider PM;DNA Commission of the International Society of Forensic Genetics 《Forensic science international》2006,157(2-3):187-197
The DNA Commission of the International Society of Forensic Genetics (ISFG) regularly publishes guidelines and recommendations concerning the application of DNA polymorphisms to the problems of human identification. A previous recommendation published in 2001 has already addressed Y-chromosome polymorphisms, with particular emphasis on short tandem repeats (STRs). Since then, the use of Y-STRs has become very popular, and a numerous new loci have been introduced. The current recommendations address important aspects to clarify problems regarding the nomenclature, the definition of loci and alleles, population genetics and reporting methods. 相似文献
73.
Recently, the ENFSI/EDNAP groups issued advice on the design of the next generation of STR multiplexes in order to encourage standardisation within Europe. As the result of collaborative experimentation within the EDNAP group, we demonstrated that the low molecular weight STRs had substantial benefits to detect degraded samples. We subsequently recommended adoption of three new mini-STR loci to improve the success rate of degraded DNA markers, concurrent with the reduction in size of the existing STR markers in current use. This also improves the discriminating power of the system which is important to improve the power of national DNA databases. Subsequent discussions have occurred with manufacturers and members of the ENFSI/EDNAP groups. Because significant time and investment is required to develop new multiplexes of 13+ STR loci, manufacturers indicated that it would be preferable to adopt a staged approach. Two differing, but parallel strategies have now emerged. The first strategy employs a 13 STR loci multiplex incorporating three mini-STRs into the current multiplex test. The second strategy employs a multiplex of six high molecular weight STRs (in current use), modified to provide smaller amplicons combined with an additional two loci of high discriminating power. Eventually, the two strategies will converge to provide a single multiplex of 15 STR loci. The process will be guided by the ENFSI/EDNAP groups. 相似文献
74.
Parson W Brandstätter A Alonso A Brandt N Brinkmann B Carracedo A Corach D Froment O Furac I Grzybowski T Hedberg K Keyser-Tracqui C Kupiec T Lutz-Bonengel S Mevag B Ploski R Schmitter H Schneider P Syndercombe-Court D Sørensen E Thew H Tully G Scheithauer R 《Forensic science international》2004,139(2-3):215-226
This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to identify possible sources of errors that could arise during the analysis and interpretation of mtDNA profiles. These findings were taken as a basis to tentatively make suitable arrangements for the construction of a high quality mtDNA database. One hundred fifty mtDNA profiles were submitted to the evaluating laboratory, and disaccording profiles were classified into four groups corresponding to the source of error: clerical errors, sample mix-ups, contaminations and discrepancies with respect to the mtDNA nomenclature. Overall, 14 disaccording haplotypes (16 individual errors) were observed. The errors included 10 clerical errors, 3 interpretation problems, 2 cases of sample mix-up and 1 case of point heteroplasmic mixture, where the 2 sequencing reactions brought inconsistent base calls. This corresponds to an error rate of 10.7% in a virtual mtDNA database consisting of the collaborative exercise results. However, this estimate is still conservative compared to conclusions drawn by authors of meanwhile numerous publications critically reviewing published mtDNA population databases. Our results and earlier published concerns strongly emphasize the need for appropriate safety regulations when mtDNA profiles are compiled for database purposes in order to accomplish the high standard required for mtDNA databases that are used in the forensic context. 相似文献
75.
76.
Preparation of degraded human DNA under controlled conditions 总被引:7,自引:0,他引:7
DNA typing through analysis of short tandem repeats (STRs) and mitochondrial DNA (mtDNA) by means of the polymerase chain reaction (PCR) and sequencing are the common methods for the forensic identification of persons and reconstruction of kinship, especially when skeletal human remains have to be analyzed. Furthermore, samples typically found at crime scenes may be both quantitatively and qualitatively inadequate since they may contain very scarce and often degraded DNA due to exposure to heat, light, humidity, and microorganisms. In order to improve the performance of STR typing technology in those cases where DNA availability is limited, it would be desirable to have a source of degraded DNA with known properties. For this purpose, we have developed a method to prepare artificially degraded DNA under controlled conditions. By treatment of genomic DNA with sonication and DNAse I we have produced DNA fragments within a defined range of lengths. STR typing of this degraded DNA with a commercially available multiplex kit could only produce partial profiles as indicated by the absence of STR alleles with sizes >200 bp. This artificially degraded DNA can be used for the improvement and standardization of STR typing protocols when only highly degraded DNA is available for analysis. 相似文献
77.
Christopher J. Schneider 《Critical Criminology》2004,12(1):87-103
This paper begins with an overview of criticalrace theory tenets, which in turn will befollowed by an overview of postmodernism. These bodies of knowledge consist of an arrayof ideologies; but for the purpose of thisarticle only the fundamentals of each will bediscussed. Thereafter, an integration ofthese two contemporary areas of thought willdemonstrate the constructive linkage ofcritical race theory and postmodern theory. Theintegrations that follow will emphasize how acollaboration of critical race theory tenetsand postmodern thought can contribute to asuccessful, and more importantly improved,analysis of the social constructions of race,class, and gender. In addition, theintersectional analysis presented willdemonstrate a more informative and betterunderstanding of the subtleties of blatant andmore hidden forms of race, class, and gender. 相似文献
78.
In the aftermath of an election it has become customary to systematically compare the predictive accuracy of polls and political stock exchanges. However, most of these analyses do not emerge from real predictions. In this research note, we compare the predictive performance of a time-series model to the accuracy of polls and a political stock market in the 2005 german Bundestag (general) election. Our analysis shows that Wahl$treet, the most significant german political stock exchange, was again closest to the actual results leaving commercial polling institutes behind. Due to the exceptionally large errors of most institutes we briefly discuss how such inaccurate forecasts might affect voting behaviour in the light of social choice theory. 相似文献
79.
Schneider J 《U.S. news & world report》2003,134(19):58,60
80.
Governance at international and global levels is not only provided through states and markets but also through a variety of private organizations. The business world is well represented through this kind of organization and contributes to global governance through self-regulation across a number of industries. This article examines these efforts in the encompassing organization of global commerce, in the pharmaceutical industry and among dye stuffs producers. Smaller organizations are generally better suited to monitor compliance and impose sanctions on members violating the codes and norms behind self-regulation. Even small organizations, however, are confronted with problems and there is also evidence of large and very complex organizations having established effective mechanisms as alternatives to public regulation. These experiences can be built into theories on self-regulation as a form of global governance. 相似文献