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11.
Our objective is to provide crime laboratories with a technique for estimating the age of a bloodstain. Toward that goal, we have used multiplexed, real-time RT-PCR (or qPCR) to determine the relative stability of different-sized segments of the same RNA species as well as differences in stability between two different RNAs' change over time in bloodstains. Our results indicate that a multivariate analysis of the changing ratio of the different RNA segments can be used to differentiate between samples of different ages in the defined population. Bloodstains from 29 of 30 donors could be partitioned into different ages using this technique. Although further improvements will be required before this approach can be implemented in crime laboratories, the multivariate analysis holds promise of providing a reliable approach for temporally linking a bloodstain to the commission of a crime or excluding a bloodstain as being irrelevant to the case in question. 相似文献
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Simultaneous separation of different types of amphetamine and piperazine designer drugs by capillary electrophoresis with a chiral selector 总被引:1,自引:0,他引:1
Bishop SC McCord BR Gratz SR Loeliger JR Witkowski MR 《Journal of forensic sciences》2005,50(2):326-335
The recent emergence of a new class of piperazine-type compounds has brought about the need for laboratory screening methods for both seized drugs and toxicological samples. These piperazine compounds, which include 1-benzylpiperazine (BZP) and 1-(3-trifluoromethylphenyl)piperazine (TFMPP), exhibit comparable physiological effects and can be substituted for the classic amphetamine-type drugs. We have optimized a chiral capillary electrophoresis (CE) separation that detects a set of 6 piperazine and 4 chiral amphetamine compounds in under 23 min using a 200 mM phosphate buffer at a pH = 2.8 with 20 mM hydroxypropyl- beta-cyclodextrin (HPbeta3CD). In addition to the above compounds, a series of "clandestine" BZP diHCl samples were also analyzed using this method to assess the ruggedness of the procedure. The novel CE separation was tailored to simultaneously detect these piperzine compounds in addition to amphetamine-type drugs. Distinct migration time and UV-spectral data were obtained for all compounds of interest. 相似文献
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Data from two independent field experiments indicate that changes in question order and context may well account for an apparently precipitous decline of interest in politics at the time of the CPS 1978 American National Election Study. Evidence from a question order experiment with the SRC/CPS feeling thermometers also suggests that such contextual artifacts may not be atypical. Indeed, because of the many changes in the content and organization of the election studies over the years, context effects represent plausible rival hypotheses for a number of inexplicable shifts and trends in the time-series. In testing these hypotheses the authors derive and validate an information-processing model of how respondents infer their political states of mind from observations of their own question-answering behavior in the survey interview. In addition, the authors illustrate the wide applicability of the model tosubstantive problems in the discipline and its implications for the survey-based paradigm in political behavior research. 相似文献
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This paper uses time series methods to test the relationship between civic affairs content and audience share for the ABC, CBS, and NBC evening news in the mid-1990s. Because these programs competed in the same market, but varied the amount of their civic affairs coverage, they present an excellent opportunity to analyze this relationship. The study uses hand-coded news abstracts and O.J. Simpson murder trial coverage as separate measures of news content. The study finds that lower levels of civic affairs content and higher levels of O.J. news are associated with greater audience share. 相似文献
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Marshall A. Bell Albert B. Bishop John D. Joscelyn John J. Marsh 《Journal of criminal justice》1974,2(1):67-72
This systems analysis of the judicial and bail processes of two, large, Ohio jurisdictions provides an empirical data base for the courts to utilize in designing, implementing, and evaluating new or modified bail and court procedures. 相似文献
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A case of accidental death resulting from an autoerotic episode involving a high abdominal ligature is reported. Pathologic findings, physical evidence, and the psychological investigation are discussed. 相似文献