A novel histological technique for distinguishing between epithelial cells in forensic casework

There are a number of forensic cases in which the identification of the epithelial cell type from which DNA originated would provide important probative evidence. This study aimed to develop a technique using histological staining of fixed cells to distinguish between skin, buccal and vaginal epithelium. First, 11 different stains were screened on formalin-fixed, wax-embedded cells from five women. Samples were analysed qualitatively by examining staining patterns (colour) and morphology (absence or presence of nuclei). Three of the staining methods – Dane's, Csaba's and Ayoub-Shklar – were successful in distinguishing skin epithelial cells from buccal and vaginal. Second, cells were smeared directly onto slides, fixed with one of five fixatives and stained with one of the three stains mentioned above. Methanol fixation, coupled with the Dane's staining method, specific to keratin, was the only technique that distinguished between all three cell types. Skin cells stained magenta, red and orange and lacked nuclei; buccal cells stained predominantly orange–pink with red nuclei; while vaginal cells stained bright orange with orange nuclei and a blue extracellular hue. This staining pattern in vaginal cells was consistent in samples collected from 50 women aged between 18 and 67. Identification of cell type from unlabelled micrographs by 10 trained observers showed a mean success rate of 95%. The results of this study demonstrate that histological staining may provide forensic scientists with a technique for distinguishing between skin, buccal and vaginal epithelial cells and thus would enable more conclusive analyses when investigating sexual assault cases.     

Factors Associated With College Students’ Responses to Rape-Disclosure Scenarios: Influence of Gender,Rape Characteristics,and Opinions About Health Professionals

ABSTRACT

Few studies examine the role of friends in victims’ decisions to seek help from health professionals. This study used a sample of college students (N = 637) to examine the factors that may influence whether students would advise a friend to seek help from health professionals. After providing an open-ended response to a vignette, students answered a series of questions about formal support providers, attitudes toward women and rape victims, and their background. Findings suggest a gender divide on the advice given, and more students who responded to an acquaintance-rape scenario recommended that a victim contact health professionals in comparison to those whose scenario depicted an intimate partner rape. Implications are discussed, focusing on the role of friends and campus policy in minimizing the impacts of victimization.     

Jealousy,intimate abusiveness,and intrusiveness

An examination of self-report scales of 160 men and 76 of their partners or former partners found significant correlations between jealousy and abusiveness (for coupled dyads) or intrusiveness (for separated dyads). Jealousy was related to borderline personality and to MCMI-II measures of Post Traumatic Stress Disorder. Rejection sensitivity leading to pathological acts, such as abusiveness and intrusiveness, is seen as originating in early insecure attachment and exposure to shaming experiences.