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951.
The majority of microfluidic devices used as a platform for low‐cost, rapid DNA analysis are glass devices; however, microchip fabrication in glass is costly and laborious, enhancing the interest in polymeric substrates, such as poly (methyl methacrylate) (PMMA), as an inexpensive alternative. Here, we report amplification in PMMA polymerase chain reaction (PCR) microchips providing full short tandem repeat profiles (16 of 16 loci) in 30–40 min, with peak height ratios and stutter percentages that meet literature threshold requirements. In addition, partial profiles (15 of 16 loci) were generated using an ultrafast PCR method in 17.1 min, representing a ~10‐fold reduction in reaction time as compared to current amplification methods. Finally, a multichamber device was demonstrated to simultaneously amplify one positive, one negative, and five individual samples in 39 min. Although there were instances of loci dropout, this device represents a first step toward a microfluidic system capable of amplifying more than one sample simultaneously.  相似文献   
952.
The purpose of this paper is to seek a better understanding of the social conditions within Japan which may be related to the increasing rates of juvenile delinquency. This trend is in sharp contrast to their overall crime patterns. In fact, as reported by Ames (1981:1), Japan has the lowest crime rate in the industrialized world, and its crime totals have declined since 1955. Within this article, we discuss youth crime trends, explain the applicability of a role relationship model, discuss the breakdown in informal social control/social relationship areas, and suggest implications for Western societies.  相似文献   
953.
954.
Outside the United States those countries sharing the common law tradition are pervasively hostile to commercialization of the bail process, whereas in the United States it is the typical approach. In some jurisdictions payment for bail is a crime; in others it is simply obstructed by various civil legal disabilities. How the American branch of the common law heritage came to deviate so strikingly from the rest on the matter of commercial bail is the topic of this article.

Beginning in the second half of the nineteenth century, courts principally in Ireland, England, and India began to act against payment to bail sureties on the concept that any indemnification of them—even partial—undermined their reliability. Irish courts took the approach that indemnified potential sureties were unreliable and should be rejected by courts. Where all potential sureties were indemnified, bail should be denied. In England courts declared agreements to indemnify sureties illegal contracts contrary to public policy, which would not be enforced by courts. While India took up the refinement of this position, England went on to declare agreements to pay bail sureties to be criminal conspiracies.

Meanwhile in the United States a circumscribed version of the position that indemnification contracts were against public policy—and therefore illegal and unenforcible—actually gained acceptance between 1870 and 1912. In 1912, however, Justice Holmes in Leary v. U.S. renounced the common law concept of bail sureties in favor of an “impersonal and wholly pecuniary” view. This terminated the anti-indemnification movement. Courts soon noted the detrimental effects of commercialism on bail.  相似文献   

955.
This article compares legal and procedural constraints that operate on United States and Costa Rican hostage negotiations. In an effort to better understand the Costa Rican police system, comparisons are drawn between United States law enforcement agencies, United States military services and the Costa Rican police system. The introductory material lays the foundation for a discussion of hostage negotiation as it is practiced in Costa Rica prior to and after hostage negotiation training.  相似文献   
956.
This article addresses the problem of generational transmission of collective memory in Hong Kong about the 1989 Tiananmen Square incident. It focuses on the young participants in the annual 4 June commemoration rallies and examines the process of mnemonic socialization that brought them into the community of 4 June commemoration. Drawing upon a rally onsite survey and in-depth interviews, this study found that many young people went through a dynamic process of gradual discovery in which various social institutions—school, family and media—played complementary roles. Their understanding of Tiananmen tended to be simplified and essentialized. Yet the loss of details through essentialization has arguably allowed them to uphold a clear-cut moral judgment regarding the event and dismiss certain memory-blurring discourses straightforwardly.  相似文献   
957.
Levels of citizen estrangement from government in the United States have risen rather consistently since the late 1960s and have reached all‐time highs in recent years. Evidence is accumulating in political science research to suggest that public administrative theory may have contributed to this trend since the Progressive Era in the early 1900s. The authors develop this thesis by arguing that administrative theory in the United States has persistently portrayed public managers as “bridge builders” who link an expertise‐challenged citizenry to government in ways that emphasize bureaucratic over democratic administration. Moreover, despite claims of yet another “new” paradigmatic shift for the field, collaborative governance scholarship to date exhibits similar tendencies. To support this argument, the authors assess the common citizen‐marginalizing tendencies of three sets of administrative reforms in American public administration: the progressive, associationalist, and polycentric heritages. They offer counterarguments to this thesis and call for critical self‐reflection by the field and a more empirically robust research agenda on this topic.  相似文献   
958.
Eoin F. McGuirk 《Public Choice》2013,154(3-4):285-313
This paper proposes and tests a mechanism through which natural resources can affect democracy. I posit that, in the presence of high natural resource rents, leaders lower the burden of taxation on citizens in order to reduce the demand for democratic accountability. The theory is corroborated using micro-level data from public opinion surveys across 15 sub-Saharan countries, in addition to country-level data on natural resource rents. Results are robust to a range of alternative specifications. A supplementary analysis reveals that, consistent with the two-period model proposed, the effects are more acute closer to national elections.  相似文献   
959.
In 1971 it was hypothesised that intergenerational value changes were taking place. More than a generation has passed since then, and today it seems clear that the predicted changes have occurred. A large body of evidence, analysed using three different approaches – (1) cohort analysis; (2) comparisons of rich and poor countries; (3) examination of actual trends observed over the past 35 years – all points to the conclusion that major cultural changes are occurring, and that they reflect a process of intergenerational change linked with rising levels of existential security.  相似文献   
960.
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