Variability of the Entire Mitochondrial DNA Control Region in a Human Isolate from the Pas Valley (Northern Spain)

Abstract: In this study, we analyzed the entire mtDNA control region in 61 unrelated individuals from the Pas Valley (Cantabria), a human isolate from northern Spain, to evaluate the suitability of this analysis to increase the power of discrimination of this locus for forensic purposes in human isolates. Low values obtained for the diversity parameters confirmed the relative isolation of this human group. The main findings of this study indicated that even the analysis of the entire mtDNA control region may have important limitations for use in forensic casework when dealing with human isolates: none of the 44 individuals who exhibited identical HVI‐HVII haplotypes could be further differentiated by analysis of segment HVIII. Nevertheless, analysis of the entire mtDNA control region proved to be useful to determine the ancestry of the samples examined, by contributing to the confirmation, and, on occasion, even to the refinement of the haplogroup assignment.     

13 STR loci frequencies in the population from Paraíba, Northeast Brazil

As part of a long-term project on Northeastern Brazilians, population genetic data were obtained from 323 unrelated individuals from the state of Paraíba. The loci studied were CSF1P0, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D18S51, D21S11, D8S1179, F13A01, F13B and LPL. Their distributions are in Hardy-Weinberg equilibrium. Forensic parameters were calculated and a comparison was made with geographically nearby populations.     

A test of the differential accuracy of the maxillary versus the mandibular dentition in age estimations of immature skeletal remains based on developing tooth length

Liversidge and colleagues developed a method for predicting the age of immature skeletal remains based on the length of developing teeth. This quantitative method combines dental data from both jaws, except for the permanent lateral incisor, and because there are reasons to suspect that these two types of data are not identical and should not be combined, it raises concerns regarding the accuracy of the technique when applied differently to each jaw. In this study, the differential accuracy of the method was test when applied to the maxillary and mandibular dentition. The test sample is comprised of 57 Portuguese subadult skeletons of known age at death. Results suggest an overall high consistency between estimates obtained from both jaws, but for the permanent dentition only. In the deciduous dentition the age estimates obtained from the maxillary teeth tend to be greater than the age estimates obtained from the mandibular pair, and the differences are significant for the incisors and canine. Additionally, ages obtained from the maxillary deciduous canine also differ significantly from true chronological age. In the permanent dentition there were no differences between the ages provided by both jaws but both the maxillary and mandibular second molars show a significant tendency to underestimate true chronological age. Although this study cannot validate completely the method presented by Liversidge and colleagues, it does provide an important test to its accuracy and calls for further research into its overall performance, particularly with respect to the results obtained from both jaws.