Minisequencing-based genotyping of Duffy and ABO blood groups for forensic purposes

Duffy and ABO blood group genetic polymorphisms were studied by minisequencing analysis of single-nucleotide polymorphisms (SNPs) at nucleotide positions--33, 125, 265, and 298 of the Duffy gene and at nucleotide positions-261, 297, 467, 646, and 703 of the ABO gene. In an Italian population sample, we found four alleles and seven genotypes for the Duffy and six alleles and 16 genotypes for the ABO systems. The lower limit for reproducible results was 200 pg DNA, with a range of up to 10 ng and an optimum at 1 ng. All of the 16 analyzed inclusive paternity tests were also consistent with parentage and two out of four inconsistencies with parentage cases were excluded by one or more SNPs. Although Duffy and ABO SNP typing show lower informativeness than most current forensic tests, their robustness, the limited population distribution of FY* Fy type, and the sensitivity of the minisequencing technology suggest that these markers can be useful in selected forensic applications.     

Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study

Many X-chromosome short tandem repeats (X-STRs) have been validated for forensic use even if further studies are needed on allele frequencies and mutation rates to evaluate the extent of polymorphism in different populations and to establish reference databases useful for forensic applications and for anthropological studies. A single multiplex reaction of seven X-STRs, which includes the DXS6789, HUMARA, DXS10011, DXS7423, HPRTB, DXS6807, DXS101 loci, is presented and their allele frequency distribution in a large population sample including 556 subjects (268 females and 288 males) analysed by five forensic laboratories of Central and Northern Italy is shown. Our results demonstrate the feasibility of a single amplification/detection reaction involving seven markers of the X chromosome, which can be fruitfully used in complex kinship analysis.     

A ‘Discreet Dance’: technologies of menstrual management in Australian public toilets during the twentieth century

Across the twentieth century, the technologies available to Australian women for managing menstruation were transformed. Products for staunching blood flow changed from bulky, re-usable rags to ‘invisible’, disposable pads and tampons. Disposal facilities changed from the humble waste bin, through to incinerators, and eventually to specialised, antibacterial ‘sanitary disposal units’. The greatest impact of these shifts was felt in public toilets: places where women must deal with private bodily functions in semi-public, communal environments. Promotional materials for menstrual products and disposal facilities promised that use of their technologies would obviate age-old menstrual taboos, emancipating women from the anxiety and mortification long associated with menstruation. This paper draws upon oral histories to argue that by the close of the twentieth century the reverse was true. Increasingly efficacious and convenient menstrual products meant that Australian women could more convincingly maintain ‘menstrual etiquette’ by keeping their monthly bleeding almost completely concealed.     

Digital Knowledge Exploitation: ICT, Memory Institutions and Innovation from Cultural Assets

There has been considerable recognition of the regional embeddedness of the knowledge-based economy and its uneven geographical incidence, with mainly urban or metropolitan areas being the crucibles of knowledge-intensive activities. Drawing from recent research conducted analysing cultural industries, the paper explores how the knowledge-based economy can be built upon, focussing on the value afforded by regional cultural diversity which offers a means of economic development and growth to peripheral regions.     

The psychological impact of burglary

Abstract

The GHQ-12 and POMS-BI questionnaires were administered to 20 victims of residential burglary and 20 matched control participants at two points in time. When assessed between one and two weeks after the crime, victims were more anxious, hostile, depressed, tired and confused, and experienced more psychological distress, in comparison with controls. When assessed for a second time one month later there was a general improvement in victims' psychological well-being but their scores still differed significantly from those of controls on almost all sub-seales of the questionnaires. The data suggest that the psychological impact of burglary is considerable and may last for an extended period of time.