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211.
Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits 总被引:1,自引:0,他引:1
Hill CR Kline MC Mulero JJ Lagacé RE Chang CW Hennessy LK Butler JM 《Journal of forensic sciences》2007,52(4):870-873
The AmpFlSTR MiniFiler polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler and Identifiler STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift. 相似文献
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Hurst CV 《Journal of forensic sciences》2012,57(4):859-865
Hispanics represent the largest and fastest growing minority in the United States. It is increasingly important to understand the skeletal morphology and regional variation within this diverse group. This research focuses on the eight cranial morphoscopic traits of Southwest Hispanics from Birkby et al. (J Forensic Sci 2008;53(1):29-33) and 18 additional traits. Frequency distributions assessed the prevalence of trait expressions in Southwest Hispanic, African-American, and European-American samples. Forward stepwise discriminant function analysis indicated the best traits for differentiating these three groups. Six of the Birkby et al.'s traits are prevalent in the Southwest Hispanic sample and the best traits to distinguish the three groups are as follows: incisor shoveling, anterior malar projection, nasal sill, oval window visualization, enamel extensions, anterior nasal spine, nasal aperture width, and alveolar prognathism. This research demonstrates the efficacy of morphoscopic traits in ancestry determinations and the utility of the aforementioned traits in discriminating Southwest Hispanics, African Americans, and European Americans. 相似文献
216.
Tuohy CH 《Journal of health politics, policy and law》2012,37(4):611-632
This article examines the cases of three health care states -- two of which (Britain and the Netherlands) have undergone major policy reform and one of which (Canada) has experienced only marginal adjustments. The British and Dutch reforms have variously altered the balance of power, the mix of instruments of control, and the organizing principles. As a result, mature systems representing the ideal-typical health care state categories of national health systems and social insurance (Britain and the Netherlands, respectively) were transformed into distinctive national hybrids. These processes have involved a politics of redesign that differs from the politics of earlier phases of establishment and retrenchment. In particular, the redesign phase is marked by the activity of institutional entrepreneurs who exploit specific opportunities afforded by public programs to combine public and private resources in innovative organizational arrangements. Canada stands as a counterpoint: no window of opportunity for major change occurred, and the bilateral monopoly created by its prototypical single-payer model provided few footholds for entrepreneurial activity. The increased significance of institutional entrepreneurs gives greater urgency to one of the central projects of health policy: the design of accountability frameworks to allow for an assessment of performance against objectives. 相似文献
217.
Lu W Rankin JG Bondra A Trader C Heeren A Harrington Pde B 《Forensic science international》2012,220(1-3):210-218
The gasoline and kerosene collected from different locations in the United States were identified by gas chromatography/mass spectrometry (GC/MS) followed by chemometric analysis. Classifications based on two-way profiles and target component ratios were compared. The projected difference resolution (PDR) mapping was applied to measure the differences among the ignitable liquid (IL) samples by their GC/MS profiles quantitatively. Fuzzy rule-building expert systems (FuRESs) were applied to classify individual ILs. The FuRES models yielded correct classification rates greater than 90% for discriminating between samples. PDR mapping, a new method for characterizing complex data sets was consistent with the FuRES classification result. 相似文献
218.
Lee O. Morgan A.A.S. Marty Johnson B.S. Jered Cornelison Ph.D. Carolyn Isaac Ph.D. Joyce deJong D.O. Joseph A. Prahlow M.D. 《Journal of forensic sciences》2019,64(2):602-606
Postmortem fingerprint collection is a routine part of many forensic death investigations. Although the production of postmortem prints is usually straight forward, several obstacles and scenarios can make the collection difficult. A common challenge occurs when finger pads are mummified. Several current techniques allow for softening and rehydration of mummified finger pads; however, despite the employment of such techniques, the production of adequate postmortem fingerprints can remain elusive. The authors present two techniques that can improve the chances of obtaining suitable fingerprints from mummified remains. The “baby powder method” involves applying a cornstarch‐based powder, such as baby powder, onto the darkened and mummified finger pads, to allow better visualization of the fingerprint detail. The “transillumination method” involves carefully dissecting away the tissues underlying the finger pad, followed by placement of a bright light source underneath the finger pad, such that the finger ridge pattern is illuminated. 相似文献
219.
Anaphylactic drug reactions are rare and often serious events. The Botulinum toxin A, marketed as BOTOX, was recently approved by the Food and Drug Administration for cervical dystonia and glabellar wrinkles, after its approved use and success with blepharospasm, strabismus, and disorders of the 7th cranial nerve. It has been well received due to its efficacy in improving facial lines. This case report documents the first death associated with a Botox-lidocaine mixture given to a woman for chronic neck and back pain. Based on the medical records, autopsy, and laboratory findings, the cause of death was determined to be anaphylaxis to the Botox-lidocaine mixture. The history, indications, off-label uses and possible future applications of Botox are reviewed as well as the uses and complications of lidocaine. Although the anaphylaxis cannot be definitively proven to be due to Botox alone, this case warns of an adverse reaction related to Botox, a drug that is rapidly expanding in range of use as well as increased usage. 相似文献
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