Selective culturing and genus-specific PCR detection for identification of Aeromonas in tissue samples to assist the medico-legal diagnosis of death by drowning

The detection of autochthonous aquatic bacteria in tissue samples from drowning cases is increasingly considered as an alternative approach to assist the medico-legal diagnosis of death by drowning. Bacteria belonging to the genus Aeromonas may be suitable candidates for this application as they are ubiquitous in natural aquatic environments but are generally not part of the human microbiota. The research aims of this study were (i) to develop a sensitive, specific and rapid screening and confirmation method for Aeromonas species in tissue samples and (ii) to evaluate aseptic sternal puncture as a post-mortem sample technique and bone marrow as an alternative matrix to provide evidence of death by drowning. The presence of Aeromonas in tissue samples was verified by cultivation using the selective media Ampicillin Dextrin Agar (ADA) and Ryan's Aeromonas Medium. The use of ADA medium was found most optimal for the sensitive, inexpensive and quick detection of aeromonads in human tissue samples. Positive culture plates were confirmed by harvesting all colonies for DNA extraction and subsequent PCR amplification using Aeromonas genus-specific primers. Aeromonads were detected in lung swab, blood and bone marrow of drowned bodies (n=3), but were negative in these three matrices for all negative controls (n=90) tested. Bone marrow proved to be a suitable alternative matrix and can be sampled post-mortem by an aseptic sternal puncture. In conclusion, this study confirms previous indications that aeromonads in cultures from blood of water bodies can be considered a potential marker for drowning. Given the fact that the number of immersed bodies (drowned and non-drowned) included in this study is statistically not significant, however, more tissue samples need to be investigated to confirm the validity of these methods to aid the diagnosis of death by wet drowning.     

Correctional officers' perceptions of the competency-based counseling training program in taiwan: a preliminary qualitative research

The purposes of this exploratory study are to present a model of the competency-based counseling training program with correctional officers in Taiwan and highlight findings from a qualitative exploration. Grounded theory procedures are used to explore 23 correctional officers' perceptions of the training program. This training program results in positive feelings about the significant learning experience, greater theoretical knowledge, and a greater understanding of the counseling work. In addition, eight themes emerging from the data pertain to the significant experiences of the training process. These themes are outlined and discussed. Implications for correctional officers' counseling training program and research questions for future study are also suggested.     

Marianne’s liberty in jeopardy? A French analysis on recent counterterrorism legal developments

ABSTRACT

This article analyses two recent French counterterrorist legislations (Law No. 2016–386 – hereafter OCT&F law and the Law No 2017–1510 – hereafter the OCT&Flaw) through the lens of distinct yet complementary theoretical frameworks. Combining the State of Exception thesis of Giorgio Agamben, the Enemy Penology as framed by Günther Jakobs as well as the more recent scholarship contributions on Pre-Crime, the article seeks to contribute to the scholarly debate on the use and the consequences of the use of criminal and administrative law in the fight against terrorism. In view of the numerous terrorist attacks that France has faced in recent years, the article aims to provide deeper knowledge of the French case by drawing substantially from the unfamiliar French scholarship. The article argues that the measures recently adopted seem to deepen the exceptional and pre-emptive logic in which potentially dangerous subjects have to be identified as “the enemy” as soon as possible in order to then be contained and dealt with.     

The organisational and geographic diversity and innovation potential of EU-funded research networks

The Journal of Technology Transfer - Public funding of research improves the systemic conditions of entrepreneurial ecosystems. It provides early-stage financing to technologies that form the basis...     

Climate Change Sceptical Frames: The Case of Seven Australian Sceptics

Climate change sceptics are known for their resistance to proactive climate response policies, especially policies aimed at restricting greenhouse gas emissions. It is often assumed that scepticism about the science behind climate change would lead directly to outright rejection of all proactive climate policies aimed at mitigating emissions and adapting to climate‐induced changes already under way. This article demonstrates the variability among the climate policy views of seven well‐known Australian climate change sceptics in the period 2007–2012. Using the lens of frame‐analysis, we unpack some key sceptic rationales and narratives. The analysis shows that sceptics share a master frame that privileges individualist‐libertarian‐progress‐social order values, which are thus likely to conflict with the values implicit in conventional climate policy remedies. The analysis also shows that sceptical pre‐occupations diverge at more detailed framing levels, with various practical concerns and fears often at the centre of sceptical argumentation     

‘My Neighbour Drinks Clean Water,While I Continue To Suffer’: An Analysis of the Intra-Community Impacts of a Rural Water Supply Project in Mozambique

Rural water planners assume the positive impacts of community water projects are spread evenly across the population. We test this assumption by looking at the distribution of benefits within communities that received handpumps in rural Mozambique. Using survey and qualitative data we analyse the characteristics of those groups who benefited from the handpumps and also explore household decision-making processes. Handpump use was determined by distance, availability of other sources, perceptions of water quality, political affiliation, and wealth. We argue that the handpumps reinforced existing social divisions related to income and political affiliation and created new geographic divisions within communities.     

How policies become contested: a spiral of imagination and evidence in a large infrastructure project

This article investigates how framing processes lead to polarization in the public debate on a large infrastructure project. Drawing on an analysis of newspaper articles about the “Oosterweel connection” in Antwerp (Belgium), it concludes that imaginative framing (appeals to emotions via symbolic language) and framing through evidence (appeals to rationality via factual language) mutually reinforce each other. Because of the mutual reinforcement, we talk of a spiralling motion. When evidence backs up appeals to the imagination, such as when facts back up metaphors, these appeals are endowed with authority and hence legitimacy. While this strengthens appeals that have been “proven” to be true, it also makes actors backing these appeals increasingly frustrated with other parties that still refuse to accept them. Because of their frustration, the former are spurred to launch new imaginative appeals conveying their anger and to seek new evidence to substantiate these new appeals. Going back and forth between imaginative appeals and appeals to evidence, all parties in a conflict develop their own vision of the contested issue and their own evidence base for the policy position. Over time, their tolerance for ambiguity decreases and the debate polarizes.