The environmental dilemma in Taiwan

While econoists and academics across the globe have praised the many advances in Taiwan’s miracle economic development since the 1950s, the costs of such rapid economic growth are quickly becoming evident in the deterioration of the environment of the island. The immense beauty of Taiwan’s landscape has been seriously harmed in many areas as a result of the rapid economic growth of the nation. Air and water pollution, soil contamination, and nuclear radiation leaks, as well as deforestation had become serious problems by the 1980s. This study gives a brief overview of the state of Taiwan’s environment today, assesses government activities to clean up the environment in Taiwan, examines the role foreign nations and citizens’ groups play in Taiwan’s environmental efforts, and gives some views about the future of the environmental movement in Taiwan.     

奶牛乳腺特异性表达人溶菌酶基因载体的构建与表达

为探讨应用转基因动物技术降低奶牛乳腺炎发病的可行性,参照人溶菌酶(human lysozyme,hLZ)基因序列(NM_000239.2)设计引物,采用RT-PCR方法从人胎盘组织中成功扩增克隆了406bp的hLZ基因成熟肽片段。应用娟姗牛酪蛋白乳腺特异表达调控元件,经过多步酶切、连接、转化、筛选、鉴定,构建成奶牛乳腺表达hLZ的载体pBCN5.2-hLZ-1.1pA-EGFP-neo(13.6kb)和pBCN-3.8-hLZ-1.1pA-EGFP-neo(12.2kb)。将构建的2个载体瞬时转染Bcap细胞,24h后可观察到EGFP的表达;通过实时定量PCR检测比较2个载体hLZ的表达丰度,结果,相对表达量分别为0.89±0.04和0.62±0.05。将线性化的pB-CN5.2-hLZ-1.1pA-EGFP-neo载体转染Bcap细胞,筛选后获得阳性细胞克隆,纯化阳性细胞总蛋白进行SDS-PAGE分析,与空白对照相比,检测到14ku的新蛋白条带。Western-blot分析中亦出现14ku的特异性条带。证实构建的载体能够正常表达hLZ基因,这为进一步研究获得自然抵抗乳腺炎的转基因奶牛奠定了工作基础。     

布鲁氏菌延胡索酸水合酶与纤连蛋白和纤连蛋白溶酶原的结合活性

依据牛型布鲁氏菌(Brucella abortus)延胡索酸水合酶(fumarate hydratase classⅠ,Fumhy)基因序列设计1对特异性引物,以牛型布鲁氏菌A19株基因组为模板,通过PCR扩增Fumhy基因的开放阅读框,连接到pMD18-T Simple载体上后进行序列测定和分析;将Fumhy定向克隆到表达载体pET-28a中,构建重组表达质粒pET28a-Fumhy,并转化至大肠杆菌BL21(DE3)中,在IPTG诱导下,进行Fumhy蛋白的表达,对表达的Fumhy蛋白进行免疫原性、与纤连蛋白和纤连蛋白溶酶原结合活性检测,研究Fumhy蛋白的免疫原性及相关的生物学活性。测序结果表明,Fumhy基因全长1 620bp,编码539个氨基酸;成功获得带有His标签的Fumhy重组蛋白His-Fumhy,大小为59ku,与预期结果相符合。Western-blot检测结果表明Fumhy蛋白具有免疫原性,且具有与纤连蛋白和纤连蛋白溶酶原结合活性。由此推测Fumhy蛋白可能参与布鲁氏菌在体内的感染过程。     

Environmental attitudes and concerns of the environmental protection bureaucrats in guangzhou,people's republic of china: Implications for environmental policy implementation

This article analyzes the questions of implementation failure in environmental policy in China. Using empirical data collected in Guangzhou, People's Republic of China, die article documents the environmental attitude of Guangzhou's Environmental Protection bureaucrats. In interpreting the data, the article seeks to assess and evaluate the present state of environmental administration in China. This article provides evidence that although China has made much progress in developing environmental legislation and a regulatory framework, the economic growth and devolution of power unleashed by reforms have also created new environmental stresses and, to some extent, reduced the regulatory capabilities of governments in China.     

Ethnicity and capital: Changing relations between China and Southeast Asia

This article seeks to debunk the persistent myth of an ethnically-based Chinese capitalism and the culturalist view of an “economic miracle” created by Asians of Chinese descent. This myth claims that Chinese entrepreneurs constitute a closed category with homogenous ethnic attributes and cultural values that have enabled them to achieve economic success. This article disputes such primordialist views and proposes an alternative analysis of the cultural economy of regionalisation and constructions of Chineseness. It deconstructs the politics of culture and identity, and argues for the need to analyse Southeast Asian Chinese entrepreneurship within specific historical, geographical, economic, political and socio-cultural contexts.     

Centre of protective custody, 6th mile Kuching-Serian road,Kuching/Sarawak

This paper begins with a theoretical perspective on privatisation which links it with systemic tendencies towards over-accumulation in the global capitalist economy. To analyse health system dynamics, the health system is conceptualised as an articulation of component sub-systems (provision, financing, treatment accessories, support services, research and product development, education and training, etc.). These sub-systems are increasingly brought within the circuit of capital, in the process re-configuring the fine structure of the system along with its built-in incentives and disincentives which interactively modulate the system's overall operating characteristics. The rest of the paper provides an update on the evolving role of the Malaysian state as provider, as financier, as investor and as regulator of the health system.     

Disciplinary labor regimes in Chinese factories

Using a survey the authors initiated in fifty-four footwear factories in China, this article investigates the extent to which Chinese workers today are subjected to coercive workplace discipline. The authors compare the management practices of state-owned and collective factories, private factories owned by mainland Chinese, and those owned by investors from Hong Kong and Taiwan. The survey selects five indicators of a disciplinary labor regime: corporal punishment, compulsory overtime, discipline vis-a`-vis bodily functions (such as toilet-going restrictions), imposition of monetary penalties, and bonding of labor through mandatory deposits.