An interpretation of the genetic polymorphism and population genetic background of Ankang Han population via a novel InDel panel

In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang City, Shaanxi Province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise F_ST values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.     

Investigation on the genetic-inconsistent paternity cases using the MiSeq FGx system

Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity cases with genetic inconsistencies, a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been collected. The mutations were subsequently evaluated using Verogen’s MPS ForenSeq^TM DNA Signature Kit and a microsatellite instability (MSI) detection kit. The result showed 98.41% (62/63) of the cases had no additional autosomal mutations even when the number of A-STRs increased to 27. As for the sex chromosomes, about 11.11% (7/63) of the cases exhibited either X-STR or Y-STR mutations. D2S1338, FGA and Penta E were the most frequent altered STRs, which suggested they might be the mutation hotspots. In addition, a male with sex chromosome abnormality was observed accidently, whose genotype might be 47, XXY, rather than MSI. Nearly 56.90% of the STR loci possessed isoalleles, which might result in higher STR polymorphisms. No Mendelian incompatibility was detected among the SNP markers, which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.2009631 .     

国内外学界对布什主义的研究情况综述

2001年1月乔治·W·布什当选美国总统后,其国家安全政策即布什主义对美国国内和国际社会逐渐产生了重大影响,引起了国内外学界的重视和研究。对布什主义的研究,其实就是对当今美国布什政府的国家安全战略的研究。研究情况各国各异,本文主要就国内外学界对乔治·W·布什总统第一任期内所推出的国家安全战略即布什主义的研究情况进行简单评述。     

构建大湄公河次区域争端解决机制的思考——以区域贸易争端解决机制模式为鉴

大湄公河次区域经济合作（以下简称GMS）于1992年由亚洲开发银行发起,涉及流域内中国、缅甸、老挝、泰国、柬埔寨和越南等6个国家。对任何贸易体制而言,无论是全球性的多边贸易体制,还是区域性的双边或周边贸易体制,争端解决机制是其贸易规则中必不可少的内容。对GMS来说同样如此。因为,无论GMS贸易自由化规则设计得如何科学合理,若没有一套有效的争端解决机制作为其实施的后盾,6国之间的贸易纠纷就无法得到及时有效的解决,     

物业管理与业主自治:物业管理业主委员会资格探析

目前,我国新兴物业管理制度下应运而生的业主委员会所发挥的自治作用并不理想,原因是多方面的。但学界争论最大的是:我国业主委员会作为业主大会中“执行机构”,没有法人资格,不是独立的民事主体,不能独立承担法律责任。又以何种身份“执行”公共物业管理事务,与物业管理公司签订民事合同?最终责任如何落实呢?争议的实质焦点有二:一是业主委员会的诉讼主体资格问题,二是业主委员会的民事主体资格及其民事责任的承担问题。由此,有学者建议:在现有业主自治管理机构——业主大会和业主委员会制度的基础上,增加业主自治管理的监督机关,引入“业主团体”的概念,建立由全体业主组成的业主团体的自治组织机构。本文重点拟对业主委员会之法律地位及完善物业管理自治组织结构并发挥其自治作用进行探讨。     

美国反倾销司法审查制度研究

美国反倾销司法审查制度是美国反倾销法的重要组成部分,它主要包括司法审查的主管机构、司法审查的范围、提起司法审查的主体以及司法的审查标准等内容,是美国三权分立,权力制衡宪法原则的体现。中国企业在应对美国的反倾销指控时,应充分利用该司法救济制度维护自己的合法权益,但同时要注意该司法审查制度存在的局限性,避免不必要的资源浪费。     

农民参与旅游资源开发的法理分析——以广西贺州昭平县黄姚古镇、桂林阳朔大榕树风景区为例

我国西部聚居着绝大部分尚未摆脱贫困的农民,开发西部旅游资源,当地农民参与权具有人权性质,应当得到立法的确认和国家优先保护。本文以广西贺州昭平县黄姚古镇、桂林阳朔大榕树风景区为例,主要分析当地农民参与权的性质和正当性。     

植物新品种权的权利限制

育种者与农民是植物品种保护法律关系中两个主要的相对利益主体。在赋予育种者专有权的同时,有必要对其权利加以适当的限制,以衡平农民和其他育种者的利益。权利的限制包括对权利内容和权利行使的限制。育种者免责和农民免责主要属于对权利内容的限制;而强制许可则是为了防止品种权人滥用权利或者不实施权利而对品种权的行使进行的一种限制。     

大熊猫IgG Fc HRP酶标二抗的制备

为获得辣根过氧化物酶(HRP)标记的抗大熊猫IgG抗体,本研究利用重组大肠杆菌表达技术对大熊猫IgG Fc片段进行了表达,获得重组蛋白后进行了小鼠免疫,抗体特异性检测,并进行了多克隆抗体的HRP标记.结果显示,在37℃、IPTG浓度为0.5mmol/L、诱导5h的条件下,可获得分子质量约为88 ku的目的蛋白;将重组蛋...     

国外少年司法恢复功能的基本内容及借鉴

我国现阶段少年司法系统的功能定位仍有所欠缺。国外少年司法系统除了完成对少年犯罪人的矫正功能外,必然要承担修复少年犯罪行为对社会各方面造成伤害的恢复功能。在了解国外少年司法恢复功能的基本内容的基础上,应以和解、家庭会议、结果复原为核心机制,创造性地发挥我国少年司法系统的恢复功能。