犬细小病毒JL-(18)1/Beagle株的分离鉴定及遗传进化分析

为研究犬细小病毒(CPV)流行株遗传变异情况,本研究从吉林省某比格犬养殖场采集疑似CPV感染致死犬的肠道组织,将病料接种至F81细胞进行病毒分离,并通过电镜形态学、PCR、血凝试验及动物回归试验进行鉴定。结果显示,所分离的这株病毒在F81细胞中产生了典型CPV细胞病变(CPE);在电镜下可见病毒呈无囊膜、直径为20 nm左右的病毒粒子;PCR鉴定证实CPV核酸呈阳性;血凝试验表明此病毒对猪红细胞具有凝集作用,血凝效价为1∶256;将这株病毒命名为JL-(18)1/Beagle。动物回归试验表明,JL-(18)1/Beagle使比格犬产生犬细小病毒感染的典型临床症状;以NS1基因进行遗传进化分析显示,JL-(18)1/Beagle与CPV-2c型分离株CPV-SH1516以及Canine/China/23/2017 NS1基因亲缘关系较近,而核苷酸相似率为100%;以VP2基因进行遗传进化分析显示,JL-(18)1/Beagle与New CPV-2b型CPV/CN/JL3/2013、CPV/CN/HB1/2013和CPV/CN/JL6/2013亲缘关系较近,核苷酸相似率为99.89%~99.94%;以VP2蛋白氨基酸进行序列分析表明,JL-(18)1/Beagle属于New CPV-2b型,与近期吉林CPV分离株相比,关键氨基酸位点无明显变化。因此,本试验从吉林省比格犬肠组织中分离到1株New CPV-2b型CPV毒株。     

Corporate crime: State-owned enterprises in China

Conclusion A decade ago, the Chinese leadership frankly acknowledged that the model of a fully planned economy, with its system of state-owned and state-run enterprises, was what Lenin had called a bureaucratic dream. 86 Today, state-owned enterprises are enjoying far more freedom to operate, and the state is trying to control them with more law and fewer plans. The use of criminal law to confront corporate crime is part of the effort to import advanced management methods from the West. Nonetheless, in the context of state ownership and Party leadership, the appropriateness of this approach is questionable.The Company Law is a new instrument to bring about fundamental changes in China's system of business organizations. These changes will help determine the scope and limits of criminal law, as applied to corporate enterprises, in the next decade. In this context, I would suggest replacing the concept ofdanwei crime with the concept of corporate or company (gongsi) crime; distinguishing thosefaren that can independently bear criminal liability from those that cannot; and clearly defining the elements of corporate offenses. Corporate criminal liability is a concept applicable when the corporation not only commits the crime but also has the legal capacity to be liable in its own right. Individual liability is still the sole principle applicable to a government agency, even if the crime is collectively committed.Given the historic context of China's socioeconomic reform, criminal law reform can advance only gradually. To insure that Western concepts fit the Chinese setting, lawmakers must make certain that every new criminal statute or regulation is enforceable even where enterprises remain closely interconnected with the state and decision-makers in publicly owned enterprises are mainly appointees of the state.I gratefully acknowledge the invitation of Daniel Prefontaine, Director of the International Centre for Criminal Law Reform and Criminal Justice Policy, Vancouver, British Columbia, Canada; and of the Hon. Josiah Wood of the British Columbia Court of Appeal, to present an earlier version of this essay at the eighth international conference of the Society for the Reform of Criminal Law, Hong Kong, December 4–8, 1994. Thanks are also due to Madeleine Sann, Director of Publication,Criminal Law Forum, for her excellent editorial comments.Postgraduate Diploma of Legal Studies, ECIPSL 1984, LL.M., Shanghai Academy of Social Science 1985; Ph.D. candidate, Simon Fraser University.     

论《外资金融机构管理条例实施细则》的修订

随着我国银行业的进一步对外开放和监管法律框架的不断完善,为促进外资金融机构的安全、健康发展,我国对原《外资金融机构管理条例实施细则》进行修订和完善。本文从新《细则》的修订入手,通过比较我国和美国监管法的不同,论述了新《细则》修订的必要性、科学性及前瞻性。     

论文化转型中公务员的法律意识

目前,中国正处在从人治文化走向法治文化的转型期,从法律文化转型的角度来看,公务员应该摒弃权力至上主义,确立法律至上意识;应该摒弃法律虚无主义,全面确立法的功能意识;应该摒弃法律工具主义,全面确立法的价值意识;应该摒弃法外特权观念,强化法的责任意识。这既是建设社会主义法治国家的基本要求,也是目前全面推进依法行政、努力建设法治政府的需要。     

试论案件侦查中的“习惯“

侦查人员要善于了解、掌握习惯的特性及种类,并为侦查活动所用。本文主要从习惯的特性、种类及意义三个方面对习惯进行了研究,对侦查实践具有一定的理论指导价值。     

论隐私权的边界--以公共信息为标准

隐私权是一种重要的具体人格权，体现了社会对人在作为独立个体角色时的尊重。但是人不可能总游离于社会角色以外，自然人的隐私和隐私权也有边界。隐私的边界不是自然人的公众人物的社会角色，而是信息的公共性或公益性。以公共利益等原则确定的公共信息来划定隐私权的边界，才能实现独立个体与群体社会的和谐统一。     

试论我国行政法院制度建构

行政法院制度起源于西方大陆法系国家并在这些国家得到了发展完善,其中以法国行政法院和德国行政法院为代表。我国引进行政法院制度,有利于建设法治国家体系、保证行政审判独立和高效解决行政纠纷。为此目的,基于国情,我国行政法院制度的建构既将有别于法国模式和德国模式,也将有别于我国普通法院制度。     

论农村土地制度的变迁

注重经济政策的调整而忽视农村土地制度的创新 ,可能是我国农业不能长期稳定增长的症结所在。文章先提出一个产权分析框架 ,揭示产权的基本内涵、主要功能以及产权制度的变迁 ;然后用这一框架分析我国农村土地制度的具体特征 ;最后 ,提出农民拥有自由转让的土地产权与坚持农村土地集体所有制是不矛盾的 ;土地规模经营的实现只能建立在产权流转的基础之上 ,当前应努力为土地产权流转创造有利条件。     

中国TBT法律体系构建与完善研究

TBT是当今国际贸易中最隐蔽、最难突破的一种贸易壁垒,已成为继配额、原产地和反倾销措施之后,影响中国对外贸易的又一项重要的非关税壁垒。中国作为WTO的成员国,应从WTO相关规则出发,提出构建与完善中国TBT法律体系的理论思考与现实探寻。     

清宫汤协助药物流产的临床与实验研究

目的 :观察清宫汤协助药物流产 (药流 )的作用 ,并探讨其机制。方法 :将 36 0例药流者随机分成单纯药流组 (不用其他任何中西药 )、催产素组 (待孕囊排出后用催产素 )、清宫汤组 (在药流当天服清宫汤 ) ,临床观察 3组出血情况 ,并进行动物实验。结果 :流产效果 :清宫汤组不全流产率明显低于单纯药流组 (P <0 .0 5 ) ;阴道出血疗效 :清宫汤组显效率及总有效率明显高于另两组 (P<0 .0 5 ) ;阴道出血量 :清宫汤组出血量少者明显多于单纯药流组 (P <0 .0 5 )。动物实验证实 ,清宫汤能增强动情小鼠体外子宫平滑肌的收缩功能 ,增加收缩频率和活动力 ,并能减轻小鼠耳郭肿胀及抑制冰醋酸所致的腹腔血管通透性增高 ,与对照组比较 ,差异有显著性 (P <0 .0 5或P <0 .0 1)。结论 :清宫汤可配合药流提高流产效果 ,减少不全流产 ,缩短出血时间 ,减少出血量 ,安全有效