Short tandem repeat (STR) genotyping of keratinised hair. Part 1. Review of current status and knowledge gaps

We present a review of the literature on procedures for obtaining short tandem repeat (STR) genotypes from keratinised hair, being either hair shaft or telogen phase (naturally shed) hairs without associated scalp, follicle or sheath cells. Both the hair shaft and the telogen hair club have been subjected to the DNA-degrading keratinisation process and are more likely to be found at a crime scene than anagen (plucked) or catagen phase hairs. We discuss human hair structure, the human hair growth cycle, the keratinisation process and their implications for DNA extraction procedures, PCR amplification strategies and the interpretation of STR genotypes. Knowledge gaps and areas requiring research are identified and are the subject of a second article in this series.     

Evaluation of the Cozart RapiScan drug test system for opiates and cocaine in oral fluid

The purpose of this study was to evaluate the efficiency of the Cozart RapiScan (CRS) drug test system for detecting opiates and cocaine in oral fluid. Oral fluid samples were collected using the Cozart RapiScan collection system from 358 donors who were receiving treatment for their addiction and were monitored for drug misuse. A further 103 oral fluid samples were collected from volunteer donors who were not drug users. The samples were analyzed in the laboratory using the two-panel Cozart RapiScan cartridge for opiates and cocaine and confirmed using gas chromatography-mass spectrometry (GC-MS). The samples were stored frozen at -20 degrees C until analysis by GC-MS. The overall accuracy of the CRS for both opiates and cocaine was 100%. Samples spiked at 50% above and below the cut-off consistently gave negative and positive results respectively. A total of 88 samples were positive for various opiates and 111 samples were positive for cocaine and/or its metabolites. The CRS for opiates and cocaine in oral fluid, using a cut-off of 30 ng/mL morphine or benzoylecgonine equivalents in neat oral fluid, had overall efficiencies of 98% and 99%, respectively, versus GC-MS. A series of potential adulterants of oral fluid were evaluated and shown not to alter the outcome of the test result.     

Validation of the Cozart microplate EIA for analysis of opiates in oral fluid

The purpose of this study was to determine the performance characteristics of the Cozart microplate EIA for detecting opiates in oral fluid from patients in a drug misuse treatment program. Oral fluid samples were collected using the Cozart RapiScan Collection System from 216 donors who were receiving treatment for their addiction and were monitored for drug abuse. A further 40 oral fluid samples were collected from volunteer donors who were not drug users. The samples were analyzed in the laboratory by using the Cozart microplate EIA for opiates and confirmed using gas chromatography-mass spectrometry (GC-MS). The samples were stored frozen until analysis by GC-MS. The intra-assay precision for the Cozart microplate oral fluid EIA for opiates over 40 assays was 0.43% to 9.13% CV (within assay) and 2.9% to 9.1% CV (within day). A total of 109 samples were positive for various opiates. The Cozart microplate EIA for opiates in oral fluid, using a cut-off of 30 ng/ml morphine equivalents in neat oral fluid, had a sensitivity of 99.1 +/- 2.1% and a specificity of 94.4 +/- 2.2% versus GC-MS. A series of potential adulterants of oral fluid were evaluated and shown not to alter the outcome of the test result.     

Regional Development of Small Firms in Poland

Employing a probit, logit and gompit model this paper demonstrates that small firm development, represented by a group of structural, behavioral and performance variables determine regional location in Poland. The paper uses original data that samples the small firm stratum in two contrasting regions, Pomorskie and Lubelskie. The following variables were shown to be significantly correlated with regional location: legal structure, subcontracting, technological level of the products of the firm, average wage and intention to expand turnover.     

International Guidelines audit tool tested in Cambodia

Until mid-2003, the audit tool developed at the Australian National University to measure compliance with the International Guidelines on HIV/AIDS and Human Rights had not been tested in a developing country. This article describes the process and preliminary findings from the application of the audit in Cambodia. Of particular interest was the willingness of the Cambodian government to permit the audit, as well as the publication and dissemination of the results.     

Developing regulatory standards for the concept of security in online dispute resolution systems

Online dispute resolution (ODR) has improved access to justice in the digital world. ODR users benefit from faster and cheaper dispute resolution mechanisms compared to traditional litigation and Alternative Dispute Resolution. There are few and quite varied regulatory systems for ODR.This research aims to develop a set of standards to measure the concept of security and to increase the consistency of security in ODR systems. An exploratory mixed method approach is used, involving a quantitative (survey) and mainly qualitative approach (face-to-face interviews) for gathering data. We identify three elements of information security, privacy, and authentication as standards for an appropriate ODR legal framework. Finally, these findings led to practical implications for policy makers and regulators.