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131.
Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) 总被引:1,自引:0,他引:1
Several assays for gamma-hydroxybutyrate (4-hydroxybutyrate, GHB) have been developed based on the enzyme gamma-hydroxybutyrate dehydrogenase (GHB-DH). Enzymatic oxidation of GHB by NAD+ is coupled to diaphorase-mediated reduction of pro-dye to yield colored product. GHB-DH from Ralstonia eutropha was cloned and expressed as a stable fusion protein easily purified by affinity chromatography. Quantitative initial velocity and endpoint versions of the assay in solution are described. Michaelis-Menten parameters for oxidation of GHB and ethanol were estimated. A semi-quantitative "dipstick" version of the assay on paper also is described. Both solution endpoint and "dipstick" assays are sensitive to about 0.05 mg GHB/mL using 10 microL of sample. Ethanol at concentrations possible in urine and agents used to stabilize physiological fluids for forensics analysis do not interfere significantly. The "dipstick" assay also allows detection of GHB in alcoholic beverages after evaporation of about one-fourth drop of beverage before testing. The enzymatic assay for GHB is reliable, sensitive, inexpensive and rapid. 相似文献
132.
Wright J Hensley C 《International journal of offender therapy and comparative criminology》2003,47(1):71-88
Although serial murder has been recorded for centuries, limited academic attention has been given to this important topic. Scholars have attempted to examine the causality and motivations behind the rare phenomenon of serial murder. However, scant research exists which delves into the childhood characteristics of serial murderers. Using social learning theory, some of these studies present supporting evidence for a link between childhood animal cruelty and adult aggression toward humans. Based on five case studies of serial murderers, we contribute to the existing literature by exploring the possible link between childhood cruelty toward animals and serial murder with the application of the graduation hypothesis. 相似文献
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Thirty-six male students, drawn from a sample of 1195, were interviewed to obtain a personal history. A battery of projective psychological tests (Rorschach and TATs) were also administered to them. The students were divided into four groups of nine each, Jewish radicals (JR), Christian radicals (CR), Jewish moderates (JM), and Christian moderates (CM), to test the significance of religious background as it related to political outlook. Eight significant psychological variables were found and defined. No differences were found between JMs and CMs. Radicals differed from moderates on three variables: negative identity, masochistic surrender, and treating people as concepts. In addition, JR subjects demonstrated consistently a wandering fantasy, flight from the mother, the mother as salient, and machismo as psychological variables. CRs were not characterized by any of these variables. As with both groups of moderates, the father of the CRs was psychologically salient, but unlike the moderates, CTs perceived their fathers as flawed. The possible dynamic meaning of these configurations is discussed, as are their possible relationship to radical behavior and radical political ideology.This study was supported by grants from the American Jewish Committee and The National Science Foundation (GS35307A).Director of Resident Education, McLean Hospital. M.D., Harvard Medical School; residency training at Boston Veteran's Administration Hospital and Beth Israel Hospital in psychiatry. Psychoanalytic training, Boston Psychoanalytic Institute. Major interest: depression.Director of Training for Psychology Interns. Ph.D., Brandeis, 1960; Master's degree, University of Illinois. Major interest: schizophrenia.Professor of Political Science, Smith College. Major interest: modern European history — applying psychoanalytic methods to historical and social problems like student activism. 相似文献
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Christopher D. DeSante 《American journal of political science》2013,57(2):342-356
Attitudes toward racialized and redistributive policies like welfare are often thought of as a function of both principled ideological positions and the underlying racial attitudes a person holds. Kinder and Sanders (1996) look at racial resentment as one explanation, while Sniderman and his colleagues look to principled conservatism and authoritarianism as viable alternatives, claiming that racial resentment is merely proxying a legitimate race‐neutral commitment to equality of opportunity. This article engages this debate through an experimental design which tests whether “hard work” is rewarded in a color‐blind manner. The experimental design also affords scholars the opportunity to separate the effects of the two components of racial resentment: principled values and racial animus. The results show that American norms and implicit racism serve to uniquely privilege whites in a variety of ways. 相似文献
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Christopher G. Salisbury 《澳大利亚政治与历史杂志》2000,46(2):153-163
As one of the initial, and arguably most devastated, casualties of the Second World War, Poland's restoration as a free and independent state was seen as a test case for the viable continuation of the victorious Grand Alliance. Yet seemingly irreconcilable differences between the Western powers and the Soviet Union over intricate territorial and political issues (with the Polish state and people caught firmly in the middle) would signal the end of the working alliance. The US collectively struggled to come to terms with this "Polish problem"– its government failing to deliver to the Poles on those democratic principles enshrined within the Atlantic Charter, and its people finding the very ideals for which they had gone to war compromised. While reflecting the unique complexities of this particular dilemma, American officialsrsquo; perceptions of the conundrum over Poland bear a ringing familiarity to future East European developments and certainly set the tone for the ensuing West-Soviet impasse there. Indeed, this author would not be alone in suggesting that the superpower struggle over Poland at the close of World War II was itself the very genesis of the Cold War conflict. 相似文献
140.
Stanley S. Surrey 《Policy Sciences》1976,7(4):505-518
The legislative process in the tax field and the administrative process for the issuance of regulatory and interpretative material by the Treasury Department are closely coordinated. The same persons and interests both inside and outside of Government are involved in both processes. There is thus continuity of expertise, understanding of what is at stake, adversary viewpoints, and Washington knowhow. The preparation of administrative material within the Treasury is a task for those technical personnel and policy officials who in varying degrees also have a voice in the preparation of the Treasury's legislative position and the legislative development of statutory language. The outside forces who watch legislative activity also closely watch administrative activity and seek to shape the latter just so they do the former. The response of the Treasury is much the same in both situations, and governed by the policies, attitudes and predilections of tax policy officials and career personnel. 相似文献