Simultaneous analysis of six amphetamines and analogues in hair, blood and urine by LC-ESI-MS/MS. Application to the determination of MDMA after low ecstasy intake

A rapid and sensitive method using LC-MS/MS triple stage quadrupole for the determination of traces of amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"), 3,4-methylenedioxyethamphetamine (MDEA), and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in hair, blood and urine has been developed and validated. Chromatography was carried out on an Uptisphere ODB C(18) 5 microm, 2.1 mm x 150 mm column (Interchim, France) with a gradient of acetonitrile and formate 2 mM pH 3.0 buffer. Urine and blood were extracted with Toxitube A (Varian, France). Segmented scalp hair was treated by incubation 15 min at 80 degrees C in NaOH 1M before liquid-liquid extraction with hexane/ethyl acetate (2/1, v/v). The limits of quantification (LOQ) in blood and urine were at 0.1 ng/mL for all analytes. In hair, LOQ was <5 pg/mg for MA, MDMA, MDEA and MBDB, at 14.7 pg/mg for AP and 15.7 pg/mg for MDA. Calibration curves were linear in the range 0.1-50 ng/mL in blood and urine; in the range 5-500 pg/mg for MA, MDMA, MDEA and MBDB, and 20-500 pg/mg for AP and MDA. Inter-day precisions were <13% for all analytes in all matrices. Accuracy was <20% in blood and urine at 1 and 50 ng/mL and <10% in hair at 20 and 250 pg/mg. This method was applied to the determination of MDMA in a forensic case of single administration of ecstasy to a 16-year-old female without her knowledge during a party. She suffered from hyperactivity, sweating and agitation. A first sample of urine was collected a few hours after (T+12h) and tested positive to amphetamines by immunoassay by a clinical laboratory. Blood and urine were sampled for forensic purposes at day 8 (D+8) and scalp hair at day 60 (D+60). No MDMA was detected in blood, but urine and hair were tested positive, respectively at 0.42 ng/mL and at 22 pg/mg in hair only in the segment corresponding to the period of the offence, while no MDA was detectable. This method allows the detection of MDMA up to 8 days in urine after single intake.     

Evaluation of preliminary isotopic analysis (13C and 15N) of explosives A likelihood ratio approach to assess the links between semtex samples

Currently, the use of isotopic ratio as corroborative evidence in criminal trials is explored. Beyond the analytical challenges that have been reported elsewhere, the crucial issue of the interpretation of analytical results in a fair and balanced way remains poorly documented. The aim of this paper is to propose a likelihood ratio approach for the evaluation of stable isotope data acquired from semtex samples. It will also lead to recommendations in relation to the acquisition of normalised international data.     

Returning to the child welfare system: Understanding the wellbeing,health, education,and placement of children who return

Using longitudinal archival Child Welfare System (CWS) data, this study describes child health and wellbeing at their initial entrance into the CWS and subsequent returns. Associations between child wellbeing, health, placement stability, and frequency of returning to the system were also investigated. Results indicated most children were healthy and developmentally on target. In addition, emotional distress, behavioral concerns, and number of placements in the CWS were significantly correlated with placement stability. Future work should utilize the use of developmental assessments to systematically and reliably document the concerns for children in the system, and investigate factors contributing to stable placements.     

Personality features of an adolescent female offending population

The incidence of adolescent offending has been shown to be different for young males and females. However, there is a lack of literature concerning adolescent female offenders, and despite research suggesting that personality factors may be linked with antisocial and criminal behaviour in adolescents and young adults; there have been a lack of studies investigating intra-sex personality differences in young female populations. The Millon Adolescent Clinical Inventory, a 160-item self-report personality measure, was administered to 26 non-offending and 28 offending adolescent females. It was found that the offending group was significantly less submissive, less conforming, more forceful, more oppositional and more likely to exhibit borderline personality traits than the control group. The offending group also had higher reported incidences of childhood abuse and family conflict, and were more prone to substance abuse, impulsive actions and suicide ideation. These initial findings suggest that personality differences may well exist between offending and non-offending adolescent females.     

Still sucked into the body image thing: the impact of anti-aging and health discourses on women's gendered identities

Health norms have changed over the past three decades, imposing more responsibility for health onto the individual. There are gendered implications of these changes which, when combined with increasing anti-aging pressures, have the potential to intensify the disciplinary relationship women have with their bodies. This paper, based upon interviews with 14 women, examines the impact of dominant health and anti-aging discourses on women's body practices, including exercise, makeup, clothing and diet, and ongoing construction of gendered subjectivity. Findings suggest that the women in this study are motivated to do particular body practices because of their concern with having a healthy and youthful ‘looking’ body. The women's stories reveal that anti-aging and health discourses function to reinforce normative bodily demands of femininity and consequently to intensify disciplinary control of their bodies. While the pressure to fight the appearance of aging is not new, the increasing association of aging with ill health, even illness, in conjunction with the promotion of health has implications for women's relationship with their bodies and sense of self.     

Cancer stories: Extracts

These extracted stories provide a taste of Claire's writing during her illness. There are plans to publish the collected writings edited by Barbara Einhorn and Christine Zmroczek.