Droplet-based optical trapping for cell separation in mock forensic samples

Optical tweezers have a wide range of uses for mechanical manipulation of objects in the microscopic range. This includes both living and static cells in a variety of biomedical and research applications. Single-focus optical tweezers, formed by focusing a laser beam through a high numerical aperture immersion objective, create a significant force, which enables controlled transport of a variety of different cell types and morphologies in three dimensions. Optical tweezers have been previously reported to capture and separate spermatozoa from a reconstituted simulated postcoital sample. We report herein the development of a simplified, more efficient cell transfer protocol that can separate and isolate both spermatozoa as well as leukocytes, with similar efficiencies as those previously reported. The new cell transfer method was used to separate sperm cells from a reconstituted mixture of spermatozoa and vaginal epithelial cells, with complete STR profiles developed from 50 cells with little evidence of contribution from the female contributor to the mixture. This modified protocol was then used to separate 21 samples of enriched leukocytes, with trapped cells ranging from 5 to 22 cells. Complete STR profiles were developed from as few as 10 leukocytes. Thus, with minimal sample preparation and a short trapping time, this method has the potential to provide an alternative to traditional differential extraction methods for separation of sperm:nonsperm mixtures while also providing versatility for separation of cells with differing morphologies.     

Radicalisation and Higher Education: Students’ Understanding and Experiences

ABSTRACT

Since 2015 universities have been placed under a legal duty of “due regard to prevent people from being drawn into terrorism.”¹ This reflects the belief in UK counter-terrorism policy that radicalisation exists and can be countered. Advice to universities is largely silent on how this duty applies to teaching. Yet many degree programmes generate lectures and seminar discussions where views of an allegedly radicalised nature could be aired. This article presents focus group research which elicits students’ understanding of radicalisation, and provides insights into their experience of debating contentious issues such as identity, community cohesion, and the causes of terrorism. We argue that students’ understanding of radicalisation is conflated with extremism and we explore students’ anxiety about debating these issues and reliance on educators to create the right environment for such discussions. Finally, the data presented here challenges some of the assumptions underpinning contemporary counter-radicalisation policy in the domain of higher education, which are premised on ideas of active grooming. We argue that this does not accord with students’ own experiences, as they regard themselves as discerning, critical thinkers rather than inherently vulnerable to manipulation by those espousing violent extremist views.     

Own-age bias in video lineups: a comparison between children and adults

Abstract

The present study investigated whether child (six–eight years of age) and adult witnesses (18–29 years of age) would exhibit an own-age bias when trying to identify targets from video lineups. One hundred and eighty-six participants viewed two filmed events that were identical, except one starred a child target and one a young adult. After a delay of two–three days each witness saw a lineup for the child and adult target. Children exhibited an own-age bias and were better at correctly identifying the own-age target from a target-present (TP) lineup and made more correct rejections for the own-age target-absent (TA) lineup. Adults however, showed a reversed own-age bias for the TP lineups as they made more correct identifications for the child target, but exhibited no bias for the TA lineups. The results suggest that differences in identification accuracy may be due to whether witness age and suspect age overlap.     

Examination of systematic variations in burglars' domain-specific perceptual and procedural skills

Abstract

This research examines systematic variations in residential burglars' domain-specific perceptual and procedural skills. Utilising a formula incorporating frequency of offending, generation of offending-related income, burglary charges, and estimated duration of burglary career, 53 experts and 53 novices were objectively identified from a sample of 209 interviews conducted with incarcerated burglars. The perceptual and procedural burglary skills of these two groups were compared and the results demonstrated important differences indicative of superior domain-specific performance for objectively classified experts. The theoretical implications of these findings are discussed, along with suggestions for application of this knowledge to crime prevention initiatives.     

Analysis of motivation in people with mild learning disabilities (mental handicap) who set fires

Abstract

Ten people with mild learning disabilities (mild mental handicap) who had all set rues and had been admitted to a hospital facility for people with challenging behaviours were interviewed about their perceptions of events, feelings and cognitions prior to and after setting fins. They were also asked to rate their excitement/upset in a series of fire-related situations. Results showed that people could identify reliably the events, feelings and cognitions prior to fire-setting but were less reliable at identifying consequences. Most commonly, people had felt angry prior to setting fires but it was also common to feel not listened to, sad or depressed. Multiple factors were relevant for most people. Some people identified the excitement of the rue as relevant and these people gave the highest ratings on the fire-related situations schedule and scored higher than controls on this schedule. Implications for treatment are discussed.     

TRIPS and paper tigers cause a stumble over inventive step

The Court of Appeal for England and Wales has revised the testfor inventive step and also considered the rights of appealunder Trade-Related Aspects of Intellectual Property Rights(TRIPS)     

EU GDPR or APEC CBPR? A comparative analysis of the approach of the EU and APEC to cross border data transfers and protection of personal data in the IoT era

This article examines the two major international data transfer schemes in existence today – the European Union (EU) model which at present is effectively the General Data Protection Regulation (GDPR), and the Asia-Pacific Economic Cooperation (APEC) Cross Border Privacy Rules system (CBPR), in the context of the Internet of Things (IoT).While IoT data ostensibly relates to things i.e. products and services, it impacts individuals and their data protection and privacy rights, and raises compliance issues for corporations especially in relation to international data flows. The GDPR regulates the processing of personal data of individuals who are EU data subjects including cross border data transfers. As an EU Regulation, the GDPR applies directly as law to EU member nations. The GDPR also has extensive extraterritorial provisions that apply to processing of personal data outside the EU regardless of place of incorporation and geographical area of operation of the data controller/ processor. There are a number of ways that the GDPR enables lawful international transfer of personal data including schemes that are broadly similar to APEC CBPR.APEC CBPR is the other major regional framework regulating transfer of personal data between APEC member nations. It is essentially a voluntary accountability scheme that initially requires acceptance at country level, followed by independent certification by an accountability agent of the organization wishing to join the scheme. APEC CBPR is viewed by many in the United States of America (US) as preferable to the EU approach because CBPR is considered more conducive to business than its counterpart schemes under the GDPR, and therefore is regarded as the scheme most likely to prevail.While there are broad areas of similarity between the EU and APEC approaches to data protection in the context of cross border data transfer, there are also substantial differences. This paper considers the similarities and major differences, and the overall suitability of the two models for the era of the Internet of Things (IoT) in which large amounts of personal data are processed on an on-going basis from connected devices around the world. This is the first time the APEC and GDPR cross-border data schemes have been compared in this way. The paper concludes with the author expressing a view as to which scheme is likely to set the global standard.     

A Rapid,Confirmatory Test for Body Fluid Identification,

We have developed a technique that allows investigators to confirm the presence of blood, semen, and/or saliva in a crime scene sample. It is a confirmatory test where multiple samples can be processed in less than an hour, and it is potentially portable, permitting samples to be processed at the crime scene. Samples at a scene giving a positive result can be further processed while those failing to do so may be ignored. There is a large and growing backlog of DNA evidence in the USA, slowing down the criminal justice system. This backlog has continued to grow despite an increase in the ability to process evidence faster. This technique uses quantum dot molecular beacons to test for tissue‐specific RNA species, identifying particular body fluids. We have demonstrated the tissue specificity of molecular beacons for blood, semen, and saliva.