Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot EZ1 Workstation

FTA Cards (GE Healthcare) have been used for more than 4 years in Denmark for the collection of buccal cells as reference samples in crime cases. Semi-automated protocols for STR typing of DNA on punches of FTA Cards are routinely used. In average, full STR profiles were generated from approximately 95% of the FTA Cards with a standard punching protocol, while partial or no STR profile were obtained from 5% of the samples. Here, the Qiagen BioRobot^® EZ1 Workstation (Qiagen) and the EZ1 DNA Investigator Kit (Qiagen) was used to extract DNA from 29 FTA Cards from which a complete STR profile was not generated with the standard punching protocol. All 29 samples were successfully typed with the AmpF?STR^® Identifiler™ PCR Amplification Kit (Applied Biosystems) and with the SNPforID 49plex SNP assay. The lowest amount of DNA that resulted in complete STR and SNP profiles was 80 pg. The STR and SNP profiles were identical to those generated from another sample collected from each of the 29 individuals.     

David and Goliath in the poll booth: group size,political power and voter turnout

ABSTRACT

This article analyses how the presence of a dominant group of voters within the electorate affects voter turnout. Theoretically, we argue that its absolute size affects turnout via increased free-riding incentives and reduced social pressure to vote within a larger dominant group. Its relative size compared to other groups within the electorate influences turnout through instrumental and expressive responses – in both the dominant and dominated groups – to the degree of electoral competition between groups. Empirical evidence from a large cross section of German municipalities is in line with these theoretical predictions. The observed effects should be taken into account when redesigning electoral jurisdictions through, for instance, municipal mergers or gerrymandering.     

德国犯罪原理的发展与现代趋势

一、导言 德国刑法是一种有体系的刑法,主要通过以判例为根据,也就是根据过去已经作出判决的真正案件来与其他法律制度加以区别.在刑法的基础中,人们不可以过高地评价这个区别,但也不可以过低地评价这个区别.一方面,德国刑法的发展,在很大程度上,不仅是通过立法和学术,而且是通过司法判决来向前推动的;<联邦最高法院刑事判例集>,一套多达50卷的汇编,是每个刑法学工作者,同时也是学生们经常使用的.但是,另一方面,我们的最高法官们不是在自由地创造法律,他们也需要以法律为根据,也需要以在一种所谓的刑法体系中加以总结的一般犯罪原理的基本原则为根据.     

Performance of the SNPforID 52 SNP-plex assay in paternity testing

The performance of a multiplex assay with 52 autosomal single nucleotide polymorphisms (SNPs) developed for human identification was tested on 124 mother–child–father trios. The typical paternity indices (PIs) were 10⁵–10⁶ for the trios and 10³–10⁴ for the child–father duos. Using the SNP profiles from the randomly selected trios and 700 previously typed individuals, a total of 83,096 comparisons between mother, child and an unrelated man were performed. On average, 9–10 mismatches per comparison were detected. Four mismatches were genetic inconsistencies and 5–6 mismatches were opposite homozygosities. In only two of the 83,096 comparisons did an unrelated man match perfectly to a mother–child duo, and in both cases the PI of the true father was much higher than the PI of the unrelated man. The trios were also typed for 15 short tandem repeats (STRs) and seven variable number of tandem repeats (VNTRs). The typical PIs based on 15 STRs or seven VNTRs were 5–50 times higher than the typical PIs based on 52 SNPs. Six mutations in tandem repeats were detected among the randomly selected trios. In contrast, there was not found any mutations in the SNP loci. The results showed that the 52 SNP-plex assay is a very useful alternative to currently used methods in relationship testing. The usefulness of SNP markers with low mutation rates in paternity and immigration casework is discussed.     

Multiplex PCR and minisequencing of SNPs--a model with 35 Y chromosome SNPs

We have developed a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification of 25 DNA-fragments and the detection of 35 human Y chromosome SNPs. The sizes of the PCR products ranged from 79 to 186 base pairs. PCR primers were designed to have a theoretical Tm of 60 +/- 5 degrees C at a salt concentration of 180 mM. The sizes of the primers ranged from 19 to 34 nucleotides. The concentration of amplification primers was adjusted to obtain balanced amounts of PCR products in 8mM MgCl2. For routine purposes, 1 ng of genomic DNA was amplified and the lower limit was approximately 100 pg DNA. The minisequencing reactions were performed simultaneously for all 35 SNPs with fluorescently labelled dideoxynucleotides. The size of the minisequencing primers ranged from 19 to 106 nucleotides. The minisequencing reactions were analysed by capillary electrophoresis and multicolour fluorescence detection. Female DNA did not influence the results of Y chromosome SNP typing when added in concentrations more than 300 times the concentrations of male DNA. The frequencies of the 35 SNPs were determined in 194 male Danes. The gene diversity of the SNPs ranged from 0.01 to 0.5.     

Estimation of the time since death: sudden increase of ambient temperature

This work presents a procedure for the postmortem interval estimation in the presence of a rapid increase of ambient temperature occurred during the cooling phase. The resulting disturbance produced on the cooling curve is proved to obey a two-exponential law and is removed from the actually measured body temperature. This yields a theoretical/modified body temperature, which enables the estimation of the time since death by means of the standard Nomogram method.     

Montesquieu's Mistakes and the True Meaning of Separation

In The Spirit of the Laws, Montesquieu concluded that a constitutionof liberty could best be achieved, and had been achieved inBritain, by assigning three essentially different governmentalactivities to different actors. He was wrong. His mistaken conclusionrested on two errors. First, Montesquieu thought that the primaryexercise of powers could durably be divided only where thosepowers differed in kind. Second, Montesquieu failed to recognizethe lawmaking character of executive and judicial expositionof existing law. This article analyzes implications of Montesquieu’smistakes for modern claims, both in Britain and in the UnitedStates, that liberty and the rule of law are promoted by separatingpower in certain contexts. In particular, this article questionsthe British Government’s recent claim that the valuesunderlying separation-of-powers theory call for removing ultimateappellate jurisdiction from the House of Lords. It also tracesMontesquieu’s influence on the American founders’attempt to separate power along essentialist lines, and considerssome sub-optimal consequences of that attempt, including thenon-delegation quandary and the emergence of an unchecked judiciallawmaker.     

The relationship between physical and sexual abuse and drug use in a sample of juvenile detainees in Florida and a sample of committed youthful offenders in Colorado

The relationships between child physical and sexual abuse and illicit drug use are little understood and underinvestigated. Data gathered from a study of youths located in two different institutions for detained (Florida) and committed (Colorado) youthful offenders permitted an examination of this issue. The results indicate the youths' physical and sexual abuse experiences are significantly and positively related to their use of illicit drugs. The implications of these findings for further research are drawn.