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311.
Greenspoon SA Sykes KL Ban JD Pollard A Baisden M Farr M Graham N Collins BL Green MM Christenson CC 《Forensic science international》2006,164(2-3):240-248
Human genome, pharmaceutical and research laboratories have long enjoyed the application of robotics to performing repetitive laboratory tasks. However, the utilization of robotics in forensic laboratories for processing casework samples is relatively new and poses particular challenges. Since the quantity and quality (a mixture versus a single source sample, the level of degradation, the presence of PCR inhibitors) of the DNA contained within a casework sample is unknown, particular attention must be paid to procedural susceptibility to contamination, as well as DNA yield, especially as it pertains to samples with little biological material. The Virginia Department of Forensic Science (VDFS) has successfully automated forensic casework DNA extraction utilizing the DNA IQ(trade mark) System in conjunction with the Biomek 2000 Automation Workstation. Human DNA quantitation is also performed in a near complete automated fashion utilizing the AluQuant Human DNA Quantitation System and the Biomek 2000 Automation Workstation. Recently, the PCR setup for casework samples has been automated, employing the Biomek 2000 Automation Workstation and Normalization Wizard, Genetic Identity version, which utilizes the quantitation data, imported into the software, to create a customized automated method for DNA dilution, unique to that plate of DNA samples. The PCR Setup software method, used in conjunction with the Normalization Wizard method and written for the Biomek 2000, functions to mix the diluted DNA samples, transfer the PCR master mix, and transfer the diluted DNA samples to PCR amplification tubes. Once the process is complete, the DNA extracts, still on the deck of the robot in PCR amplification strip tubes, are transferred to pre-labeled 1.5 mL tubes for long-term storage using an automated method. The automation of these steps in the process of forensic DNA casework analysis has been accomplished by performing extensive optimization, validation and testing of the software methods. 相似文献
312.
A 10-year retrospective study of pediatric toxicologic deaths was performed at the Medical University of South Carolina (Charleston, SC) from January 1989 to December 1998. During this time, 709 pediatric forensic autopsies were performed on children younger than 18 years of age. Eleven deaths were determined to be secondary to toxic exposures (excluding carbon monoxide poisonings secondary to fires). The remaining deaths were reviewed for the presence of alcohol or illicit drugs. The 11 toxicologic deaths were analyzed for age, sex, race, type of toxic exposure, cause and manner of death, location of incident, witness, and, in the younger age group, the primary caregiver at the time of exposure. The deaths had a bimodal age distribution (6 deaths in victims ages 15 to 17 and 5 deaths in victims ages 4 or younger), involving a wide range of toxins. The teenage group was composed of five males and one female, all white. The preschool group had three females and three males, all black. The manner of death ranged from accidental to suicidal to homicidal. In addition, in eight neonatal and fetal deaths, the victims tested positive for maternal cocaine use, and five of these victims tested positive for cocaine or benzoylecgonine. However, the cause of death was not stated to be cocaine in any of these neonatal and fetal cases. 相似文献
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Jacques Lacan's Seminar on The PurloinedLetter gives centrality to the operation ofthe signifier in the construction of thesubject, rather than to any `content' thatmight pertain to the signifier. In Lacan'saccount, the trajectory of the signifier isorganised around three intersubjectivepositions: seeing, not seeing and seeingoneself not being seen. This article, in anexamination of the operation of `the nation' asan origin for legal authority in the 1998Belfast Agreement, will explore the relevanceof Lacan's insight to the questions ofsovereignty and authority thrown up by legaltransition in Northern Ireland. In particular,this article will explore the way in whichnation is `suspended' as a foundation of legalauthority from the administrative discourse ofthe Belfast Agreement, but remains a potentpoint of legal and political identification.This will be done by treating `the nation' notin terms of its capacity to represent thefoundation of legal authority, but as asignifier whose displacement from `grand'aspirational constitutional discourse to themore `banal' administrative discourse of theBelfast Agreement operates to reconfigure legaland political identification. 相似文献
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