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101.
Nelson MS Levedakou EN Matthews JR Early BE Freeman DA Kuhn CA Sprecher CJ Amin AS McElfresh KC Schumm JW 《Journal of forensic sciences》2002,47(2):345-349
Quality assurance samples submitted from the NCSBI as part of a contract with TBTG to outsource DNA Database samples showed unexpected discrepancies for the locus D16S539 when all other loci yielded identical results. Discrepancies observed included allele drop out and an imbalance in sister alleles with samples returned from TBTG. This led to a comprehensive review of the technical procedures used between the two laboratories to determine the cause of the discrepancies noted for the locus D16S539, since both laboratories were using the PowerPlex 1.1 typing kit from the Promega Corporation. The NCSBI and the TBTG utilize different extraction methods (organic extraction vs. FTA) and amplification conditions (AmpliTaq vs AmpliTaq Gold), respectively, so the exact cause of discrepancy observed was not immediately apparent. Experiments at the NCSBI associated the observed allele drop out and the imbalance of the sister alleles with the use of AmpliTaq Gold and a hot start procedure. Sequencing data revealed that a point mutation resides on the D16S539 primer-binding site that reaches polymorphic levels in African-American populations. This led to the development of a degenerate primer by the Promega Corporation to detect "missing" alleles when AmpliTaq Gold is used. The degenerate primer was then thoroughly tested to show its efficacy in detecting the "true" D16S539 profile when used. 相似文献
102.
The development of less-lethal technologies has provided law enforcement personnel with an alternative to lethal force. Although the less lethal projectile was produced to engender non-penetrating wounds, case studies show that there have been a number of reported penetrating injuries ranging from minor to significant in morbidity. The objective of this study was to determine the energy per unit area required to penetrate various regions of the body. Eight unembalmed postmortem human specimens were procured for this testing. Each specimen sustained a maximum of 25 impacts consisting of shots to the anterior and posterior thorax, abdomen, and legs. A 12-gauge, fin-stabilized, rubber rocket round was used as the impactor for all of the conducted tests. The energy density required for 50% risk of penetration varied from 23.99 J/cm2 for the location on the anterior rib (p = 0.000) to 52.74 J/cm2 for the location on the posterior rib (p = 0.001). 相似文献
103.
Darren J. Monroe M.D. John T. Meehan IV M.D. Cynthia A. Schandl M.D. Ph.D. 《Journal of forensic sciences》2015,60(6):1633-1636
A 31-year-old man with migraine-induced syncope and bradycardia with subsequent pacemaker implantation died unexpectedly. Clinically unsuspected cardiac anomalies were found at autopsy including myocardial bridging of the left anterior descending artery and shelf-like coronary artery ostia. Nortriptyline was identified by toxicologic analysis. A review of the autopsy findings, the historical information, and the effects of the possible arrhythmogenic circumstances is undertaken and the potential contributions to the death are discussed. Cardiac arrhythmias have been documented during migraines. Coronary artery bridging has been known to lead to ischemia and infarction, ventricular tachycardia, and sudden death; however, these are very rare sequelae. Congenital coronary artery anomalies have been linked to sudden cardiac death, but only rarely cause death in people younger than 31 years. Migraines and the autopsy findings described have been associated with cardiac arrhythmia and sudden death. Altogether, they led to the unexpected death of this young man. 相似文献
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Cynthia Hewitt de Alcántara 《Development in Practice》1995,5(1):61-63
The World Summit for Social Development, to be held in Copenhagen in March 1995, will consider three closely inter-related themes: decreasing poverty, reducing unemployment, and enhancing social integration. With the support of the United Nations Development Programme, the United Nations Research Institute for Social Development (UNRISD) has been mobilising its worldwide network of scholars, activists, and development practitioners to promote reflection on major social issues, channel new information and ideas into the Summit preparatory process, and build public support for a successful Summit. To illustrate some of this work, we reproduce below the Summary of a Briefing Paper written by Cynthia Hewitt de Alcántara. 相似文献
106.
Huge changes are taking place in international diplomacy which have created an opening and a certain appreciation for the contributions that can be made by private citizens to international peacemaking. This article provides a snapshot of diplomatic thinking about Track II/nonofficial diplomacy, from interviews with distinguished American diplomats. As thoughts of two-way collaboration develop, it becomes increasingly important to be clear about the limits and potentially unique contributions of Track II. Open respectful dialogue between Tracks I and II is also necessary to overcome past resentments and to develop mechanisms by which the skills and insights of Track II can be fed into Track I, with greater effectiveness and integrity. 相似文献
107.
Cynthia A. Roberts 《欧亚研究》1995,47(8):1293-1326
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Cynthia Grant Bowman 《Family Court Review》2023,61(1):102-115
Cohabitants and LATs (couples who “live apart together”) do not fit the traditional categories and rules of our family law system. This article describes what we know about both cohabitants and LATs in the U.S., compares the two institutions, and makes recommendations about legal reforms with respect to each. For cohabitants the reforms would assimilate cohabiting couples to marriage after the passage of time, based on the evidence of their economic interdependence and the probability that there are children in their households. As to LATs, the reforms proposed are limited to those that would support the caretaking functions LATs perform for each other, resulting in benefits to society as a whole. 相似文献