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In malignant hyperthermia, myophosphorylase reaction shows characteristic changes that take place within minutes: (1) a generally strongly weakened reaction; (2) numerous negatively reacting fibres; (3) frequently, fibre sections that show spotty and/or striatal weak or negative reactions and fibre sections with strong striatal reactions with relatively narrow sarcomere spacings (a "sign of hypercontraction"). Obviously, the morphological findings that show characteristic "striated fibres" are typical of the malignant hyperthermia syndrome! It is important to note that the muscular fibres showing such changes are, as a rule, inconspicuous when using other stains and reactions. These pathological myophosphorylase reactions were observed in five deceased patients (one independently of anaesthesia after an extended walk) and in 19 pigs (18 times after halothane testing and once in an experimental animal with clinical evidence of the presence of malignant hyperthermia). These reactions were not noted in pigs with negative halothane reactivity or prior to halothane testing. They were also not seen in a large number of very different healthy and diseased control and reference cases from our biopsy and autopsy material. Myophosphorylase reaction enables convincing demonstration of malignant hyperthermia, past or present. Hence, it is possible to elucidate puzzling deaths or verify apparently clear death occurring during or subsequent to anaesthesia or simply following stress ("human stress syndrome"). Many of these deaths doubtlessly escape the attention of clinicians using the usual morphological examination methods. However, the reaction cannot be used to identify potential victims. 相似文献
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Changes in the wording of core measures of political attitudes in the American National Election Studies have generated a good deal of controversy about artifactual sources of change in the U.S. electorate. This research, based on several field experiments and replications, investigates the effects of using or not using various types of opinion filter questions that have appeared in the SRC/CPS series over the years. The analysis shows that the use of a filter interacts significantly with a respondent's level of education and interest in politics, particularly the latter, in determining whether a respondent will offer an opinion on a given public policy issue. But the study also demonstrates that such an interaction occurs primarily when the question about interest in politics is askedafter, rather thanbefore, a set of political issue items. In interpreting these order-and-context effects the authors develop a self-perception model of how respondents infer their interest in politics from information that isavailable in memory about their own behavior in the survey interview. 相似文献
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