1H NMR analysis of GHB and GBL: further findings on the interconversion and a preliminary report on the analysis of GHB in serum and urine

A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH.     

Does carcass enrichment alter community structure of predaceous and parasitic arthropods? A second test of the arthropod saturation hypothesis at the Anthropology Research Facility in Knoxville, Tennessee

In a second test of an arthropod saturation hypothesis, we analyzed if the on-campus Anthropology Research Facility (ARF) at the University of Tennessee, Knoxville, with its 20+ yr history of carcass enrichment, is comparable to non-enriched sites in community structure of predatory and parasitic arthropods that prey upon the sarcosaprophagous fauna. Over a 12-day period in June 1998, using pitfall traps and sweep nets, 10,065 predaceous, parasitic, and hematophagous (blood-feeding) arthropods were collected from freshly euthanized pigs (Sus scrofa L.) placed at ARF and at three surrounding sites various distances away (S2-S4). The community structure of these organisms was comparable in most paired-site tests with respect to species composition, colonization rates, and evenness of pitfall-trap abundances on a per carcass basis. Site differences were found in rarefaction tests of both sweep-net and pitfall-trap taxa and in tests of taxonomic evenness and ranked abundances of sweep-net samples. Despite these differences, no evidence was found that the predatory/parasitic fauna at ARF was impoverished with fewer but larger populations as a result of carcass enrichment. Comparison of the sarcosaprophagous and predatory/parasitic faunas revealed a tighter (and more predictable) linkage between carrion feeders (sarcosaprovores) and their carrion than between carrion feeders and their natural enemies (predators and parasitoids), leading us to conclude that ARF is more representative of surrounding sites with respect to the sarcosaprovore component than to the predatory/parasitic component within the larger carrion-arthropod community.     

Utilizing the urinary 5-HTOL/5-HIAA ratio to determine ethanol origin in civil aviation accident victims

Specimens from fatal aviation accident victims are submitted to the FAA Civil Aerospace Medical Institute for toxicological analysis. During toxicological evaluations, ethanol analysis is performed on all cases. Care must be taken when interpreting a positive ethanol result due to the potential for postmortem ethanol formation. Several indicators of postmortem ethanol formation exist; however, none are completely reliable. The consumption of ethanol has been shown to alter the concentration of two major serotonin metabolites, 5-hydroxytryptophol (5-HTOL) and 5-hydroxyindole-3-acetic acid (5-HIAA). While the 5-HTOL/5-HIAA ratio is normally very low, previous studies using living subjects have demonstrated that the urinary 5-HTOL/5-HIAA ratio is significantly elevated for 11-19 h after acute ethanol ingestion. Recently, our laboratory developed and validated an analytical method for the simultaneous determination of both 5-HTOL and 5-HIAA in forensic urine samples using a simple liquid/liquid extraction and LC/MS/MS and LC/MS/MS/MS. In this previous work a 15 pmol/nmol serotonin metabolite ratio cutoff was established in postmortem urine, below which it could be conclusively determined that no recent antemortem ethanol consumption had occurred. In the current study this newly validated analytical method was applied to five ethanol-positive aviation fatalities where the origin of the ethanol present could not previously be conclusively determined. In four of the five cases examined the detected ethanol was demonstrated to be present due to postmortem microbial formation, and not consumption, even though some indication of ethanol consumption may have been present.     

A test of sex determination from measurements of chest radiographs

Sexually dimorphic distinctions within the human thoracic area may include morphological as well as metric differences in the sternum and 4th rib. This research assesses the validity of a set of previously published measurements from chest radiographs and their use in contemporary forensic situations. The chest plates from 130 adult individuals of a known sample undergoing medico-legal post-mortem examination were examined at autopsy. Thoracic radiographs were taken using a Faxitron cabinet X-ray machine at 40 kV using Kodak Diagnostic Film Ready Pack X-Omat TL. Measurements were taken to the nearest millimetre using a sliding calliper. Logistic regression analysis of measurements of the sternum and 4th rib was undertaken to determine sex. Using 4th rib width and sternal area, sex was predicted at an accuracy of 95.8% for males and 90.3% for females.     

Assessing age-related morphology of the pubic symphysis from digital images versus direct observation

The increasingly global role of a forensic anthropologist necessitates a proper means for archiving evidence for re-examination. Large quantities of evidence can be stored and be made readily accessible through digital imaging. This study focuses on age assessment from digital photographs for personal identity reconstructions. A comparison of 52 Suchey-Brooks scores assigned to digital images and actual bone revealed that age assessment from digital images can be completed with accuracy. Coefficients of concordance imply that there significant agreement between osteological assessment of aging criteria from digital images and direct observation-greater than random change alone (p < 0.05). However, assessments from images should be approached with caution since there are inherent limitations of the naked eye in identifying morphological changes in certain skeletal features, especially where older adults are concerned. Although there is no replacement for a hands-on physical assessment, a digital archive may facilitate the global needs of the forensic anthropologist.     

How long after waterproofing a deck can you still isolate an ignitable liquid?

Dried, treated wood was sealed with Thompson's WaterSeal "Clear Multi-Surface Waterproofer" and exposed to outdoor, summer conditions. Sections of the sealed wood were then periodically tested and analyzed in accordance with ASTM methods. The WaterSeal contained a medium petroleum distillate (MPD) as a solvent for a wax. The treated lumber contained a background of aldehydes that could mimic a dearomatized MPD if not carefully investigated by the analyst. The MPD of the WaterSeal was detectable 14 days, but not 27 days after application with exposure to relatively hot, dry and sunny weather conditions. The test was repeated with the MPD detectable 16 days, but not 20 days after application with exposure to cooler and wetter weather conditions. The testing demonstrates the need for a thorough and complete investigation by the fire investigator and the submission of comparison samples to the laboratory.