瑞应寺 三百年东藏圣地

藏传佛教文化以其令人炫目的历史穿透力,赋予阜新这块土地独具魅力的民族宗教风情,数百年来滋养着这里纯朴而热情的人们,也深深吸引着世界各地无数信众、游客虔诚与向往的脚步。     

猪肺炎霉形体p52基因重组腺病毒的构建及其免疫特性

为构建携带猪肺炎霉形体(Mycoplasma hyopneumoniae,Mhp)p52基因的重组腺病毒,从Mhp J株扩增p52基因,克隆到穿梭载体pShuttle-CMV中,经Pine Ⅰ线性化后,电转化入BJ5183菌内,与其含有的骨架载体pAdEasy-1同源重组,再与脂质体混合转染AD-293细胞,包装成完整的腺病毒,经RT-PCR、间接免疫荧光鉴定后扩增,收集病毒液,免疫BALB/c小鼠,并分析了体液免疫、黏膜免疫和细胞免疫效果.结果表明,重组质粒pShuttle-CMV-P52插入片段为1 202 bp;同源重组成功;重组腺病毒可以正确表达目的蛋白,效价达1×106TCID50/mL.重组病毒经肌肉注射和滴鼻均可诱导小鼠产生血清和肺匀浆液特异性IgG、SIgA,但不能诱导特异的淋巴细胞增殖.证实,成功构建了表达p52基因的重组腺病毒,该病毒可诱发小鼠产生特异的体液免疫和黏膜免疫,但不产生细胞免疫应答.     

重组鸡痘病毒rFPV-VP3-F的遗传稳定性及在鸡体内的表达

为了评价重组禽痘病毒rFPV-VP3-F的遗传稳定性,将rFPV-VP3-F在鸡胚成纤维细胞(CEF)连续培养20代.对第5、10、15和20代病毒的F基因进行扩增及序列分析,未发现F基因发生氨基酸改变;通过间接免疫荧光方法检测各个代次rFPV-VP3-F中F基因特异表达的情况;将rFPV-VP3-F和禽痘病毒FPV017分别接种于35日龄商品鸡,在免疫后第7、14、21、28、35 d采集各组血清,检测中和抗体效价.结果显示,F基因在重组禽痘病毒中可以稳定遗传,可在CEF中表达相应蛋白,可刺激鸡产生相应的抗体.     

The dynamic political economy of support for Barack Obama during the 2008 presidential election campaign

In recent years, students of voting behavior have become increasingly interested in valence politics models of electoral choice. These models share the core assumption that key issues in electoral politicds typically are ones upon which there is a widespread public consensus on the goals of public policy. The present paper uses latent curve modeling procedures and data from a six-wave national panel survey of the American electorate to investigate the dynamic effects of voters’ concerns with the worsening economy—a valence issue par excellence—in the skein of causal forces at work in the 2008 presidential election campaign. As the campaign developed, the economy became the dominant issue. Although the massively negative public reaction to increasingly perilous economic conditions was not the only factor at work in 2008, dynamic multivariate analyses show that mounting worries about the economy played an important role in fueling Barack Obama’s successful run for the presidency.     

国有企业社会责任实现路径探析

国有企业社会责任与一般企业社会责任有着根本区别,是目前推进我国企业社会责任的关键问题。国有企业由于其本身＂公益性＂与＂营利性＂并存的性质,决定了其社会责任的内涵具有特殊性。应该通过产权制度明晰化、社会责任内涵法制化、评价体系科学化、监督主体独立化和多元化等多种渠道来确保国有企业社会责任的有效实现。     

Impact of the US Credit Crunch and Housing Market Crisis on China

Similarities between the US, the UK and the Chinese housing markets, including the movements of interest rates and house prices, and the exposure of some Chinese banks to the US mortgage securitization market, have triggered concern about whether China could experience a US-style credit and housing market crisis. Significant differences between China and Western economies make that unlikely in the near future. China's booming house market has been supported by fast economic growth, rapid urbanization and high domestic savings. Chinese banks have also been less exposed to mortgage defaults than their Western counterparts. However, the relative underdevelopment of the financial system—credit monitoring and asset securitization—may expose China to domestic mortgage lending-related crises.     

放线杆菌外膜蛋白酵母双杂交诱饵质粒的构建及自激活与毒性检测

为了筛选胸膜肺炎放线杆菌外膜蛋白在肺泡上皮细胞上的作用受体,应用酵母双杂交技术构建诱饵质粒载体pGBKT7-omp(胸膜肺炎放线杆菌外膜蛋白),从胸膜肺炎放线杆菌提取基因组DNA,用PCR方法获得omp,克隆于pGBKT7载体上,进行酶切鉴定及序列分析,并检测pGBKT7-omp在酵母双杂交系统中的自激活和毒性作用。结果表明,成功构建了诱饵质粒载体pGBKT7-omp,并证明其在酵母双杂交系统中无自激活及毒性作用。表明pGBKT7-omp可应用在酵母双杂交系统中,用于寻找猪肺泡上皮细胞cDNA文库中与omp相互作用的蛋白质。     

Population genetics for Y-chromosomal STRs haplotypes of Chinese Tibetan ethnic minority group in Tibet

Y-chromosomal STRs loci were analyzed from a sample of 119 healthy unrelated autochthonous male individuals of Chinese Tibetan ethnic minority group using a multiplex PCR system. Allele and haplotype frequencies for DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393, DYS385a,b, DYS438, and DYS439 were determined by the Y-PLEXtrade mark 12 kit. The gene diversity values for the Y-STRs loci ranged from 0.3347 (DYS438) to 0.9547 (DYS385a,b). A total of 110 haplotypes were identified in the Y-STR loci, among which 104 were unique, while six occurred more than once. The overall haplotype diversity for the Y-STRs loci was 0.9981, and the discrimination capacity was 0.9897. The results in the present study can be used for routine forensic application in the region, and enrich Chinese ethnical genetic informational resources.     

和谐社会呼唤立法平等

和谐社会的核心问题是协调好人与人的利益关系,利益关系在法律上表现为权利义务关系。当前我国出现一些不和谐现象的一个重要原因是立法上的权利义务分配不均。只有立法贯彻普遍平等的原则,赋予每个人平等的权利和义务,做到立法平等,才能使我们的社会利益趋于均衡,实现社会和谐。