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51.
Law and Human Behavior - How might the deconstruction of the legal theory of competence be related to modern neuropsychological models of cognition? To address this question, we examined... 相似文献
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Robert S. McLaren Martin G. Ensenberger Bruce Budowle Dawn Rabbach Patricia M. Fulmer Cindy J. Sprecher Joseph Bessetti Terri M. Sundquist Douglas R. Storts 《Forensic Science International: Genetics Supplement Series》2008,2(4):257-273
Several laboratories have reported the occurrence of a split or n − 1 peak at the vWA locus in PowerPlex® 16 and PowerPlex® ES amplification products separated on 4- and 16-capillary electrophoresis instruments. The root cause of this artifact is post-PCR reannealing of the unlabeled, unincorporated vWA primer to the 3′-end of the tetramethylrhodamine (TMR)-labeled strand of the vWA amplicon. This reannealing occurs in the capillary post-electrokinetic injection. The split peak is eliminated by incorporation into the loading cocktail of a sacrificial hybridization sequence (SHS) oligonucleotide that is complementary to the vWA primer. The SHS preferentially anneals to the primer instead of the TMR-labeled strand of the vWA amplicon. In addition, the n − 10/n − 18 artifact that may be seen at the vWA locus was determined to be due to double-stranded amplicon formed post-electrokinetic injection into the capillary. This was also eliminated by adding in two Complementary Oligo Targets (COT1 and COT2) in addition to the SHS oligonucleotide into the loading cocktail. These three oligonucleotides are complementary to the 33 bases at the 5′-end of the unlabeled vWA amplicon strand and the 60 bases at its 3′-end and therefore compete for hybridization to the TMR-labeled amplicon strand. Incorporation of these three oligonucleotides in the Internal Lane Standard 600 (ILS600) eliminate both the split peak and n − 10/n − 18 artifact in PowerPlex® 16 and PowerPlex® ES amplification products without affecting sizing of alleles at the vWA locus or any locus in the PowerPlex® 16, PowerPlex® Y, PowerPlex® ES, AmpFlSTR® Profiler Plus® ID, AmpFlSTR® Cofiler®, and AmpFlSTR® SGM Plus® kits. 相似文献
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Oliver Turner 《The Political quarterly》2019,90(4):727-734
Since 2016, the UK government has outlined plans for ‘Global Britain’ as a framework for post‐Brexit foreign policy. Some criticise the idea as a vision of ‘Empire 2.0’, but it is rarely made clear exactly what form it takes or what its wider political implications are. This article argues that Global Britain constitutes not just an idea or a slogan, but a foreign policy narrative and, more specifically, the narrative of empire. Indeed, to appear reasonable its grand ambitions require pre‐existing knowledges of past imperial ‘successes’ and accepting images of empire among the British public. Yet Global Britain lacks efficacy: as a domestic rather than an international narrative, by being inherently regressive in its worldview, and for contradicting the preferences of international partners on which the UK heavily relies. These narrative flaws, it is argued, make Global Britain an actively problematic, rather than merely ineffective, component of UK foreign policy. 相似文献
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Oliver Dowlen 《Political studies》2009,57(2):298-315
The central argument of this article is that it is possible to identify one major or primary potential that sortition brings to the political community when it is used to select office holders. This is to be found when sortition is used in such a way as to maximise its most essential feature – its arationality – and where such an application has the most significant and positive impact on the political process and the political community. In such applications the advantages of using an arational process can be seen as outweighing its disadvantages. In political practice – especially in a republican context – this primary political potential is the ability of sortition to protect the public process of selection from subversion by those who might wish to use it for their own private or partisan ends. This helps to defend the polity from those seeking to exercise unconstitutional or arbitrary power – either in the form of a single tyrant or of factions vying for partisan control. In addition, sortition can produce a series of secondary benefits to the republican polity: the polity can be understood as impartial, the threshold to citizen participation can be lowered and the model of the independent citizen encouraged. These benefits, however, can be seen as deriving from initial protection of the process of selection from manipulation – a quality of lot which is present whatever the motivation of those instigating a particular lottery scheme. Although the political use of lot cannot be confined to the protection of open government, its potential to limit the power of individuals or covert groupings makes it naturally commensurate with this role. 相似文献